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  4. Mouse Alpha-fetoprotein,AFP ELISA Kit

Mouse Alpha-fetoprotein,AFP ELISA Kit

  • 中文名稱：
    小鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯免疫試劑盒
  • 貨號：
    CSB-E08282m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯免疫試劑盒（CSB-E08282m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的AFP含量。AFP即甲胎蛋白，是一種糖蛋白。其正常情況下主要由胎兒肝細胞及卵黃囊合成。在成人中，AFP升高常見于肝癌等惡性腫瘤。研究上常將其作為腫瘤標志物，通過檢測其水平輔助肝癌等疾病的診斷、監測及預后評估。試劑盒檢測范圍為1.23 ng/ml-900 ng/ml.，靈敏度為1.23 ng/ml.。科研人員可通過探究肝損傷修復、腫瘤發生發展或胚胎發育相關機制，為構建疾病模型、評估干預效果提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測范圍：
    1.23 ng/ml-900 ng/ml.
  • 靈敏度：
    1.23 ng/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    34.056

    34.846

    35.720

    33.819

    34.253

    34.648

    SD

    0.035

    0.038

    0.042

    0.036

    0.042

    0.053

    CV(%)

    4.481

    4.744

    5.103

    4.645

    5.344

    6.658

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    86

    Range %

    82-93

    1:2

    Average %

    98

    Range %

    94-104

    1:4

    Average %

    99

    Range %

    96-108

    1:8

    Average %

    88

    Range %

    80-100

  • 回收率：

    The recovery of mouse AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    83-97

    EDTA plasma (n=4)

    96

    92-105

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    900

    2.379

    2.252

    2.316

    2.161

    300

    1.975

    1.876

    1.926

    1.771

    100

    1.305

    1.304

    1.305

    1.150

    33.33

    0.812

    0.779

    0.796

    0.641

    11.11

    0.534

    0.494

    0.514

    0.359

    3.7

    0.372

    0.349

    0.361

    0.206

    1.23

    0.264

    0.249

    0.257

    0.102

    0

    0.161

    0.149

    0.155

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Binds estrogens, fatty acids and metals.
  • 基因功能參考文獻：
    1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
    2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
    3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
    4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
    5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
    6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
    7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
    8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
    9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
    10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
    11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
    12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
    13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
    14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
    15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
    16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 組織特異性：
    Plasma.
  • 數據庫鏈接：


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