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  4. Mouse Alpha-enolase(ENO1) ELISA kit

Mouse Alpha-enolase(ENO1) ELISA kit

  • 中文名稱：
    小鼠烯醇酶1(ENO1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL007670MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠烯醇酶1(ENO1)酶聯免疫試劑盒（CSB-EL007670MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ENO1含量。ENO1即烯醇化酶1，是糖酵解途徑中的關鍵酶。研究發現，它在腫瘤細胞中高表達，與腫瘤的生長、侵襲和轉移相關。其機制可能是通過調節糖代謝，為腫瘤細胞提供能量，同時影響腫瘤微環境等，有望成為腫瘤治療的新靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于體外定量分析小鼠模型或細胞實驗中ENO1的動態表達變化，可為代謝通路研究、腫瘤生長微環境分析、炎癥反應機制探索等基礎科研領域提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Eno1 ELISA Kit; Eno-1 ELISA Kit; Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 98
    Range % 94-103
    1:2 Average % 85
    Range % 81-90
    1:4 Average % 104
    Range % 100-107
    1:8 Average % 85
    Range % 80-90
  • 回收率：
    The recovery of mouse ENO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 107 103-110
    EDTA plasma (n=4) 86 83-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.557 2.601 2.579 2.416
    2000 2.291 2.186 2.239 2.076
    1000 1.553 1.562 1.558 1.395
    500 0.981 0.998 0.990 0.827
    250 0.582 0.571 0.577 0.414
    125 0.414 0.432 0.423 0.260
    62.5 0.295 0.290 0.293 0.130
    0 0.161 0.164 0.163 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
  • 基因功能參考文獻：
    1. this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection PMID: 28525970
    2. Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions. PMID: 27460355
    3. The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
    4. Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels PMID: 26302382
    5. a Wnt/beta-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair, is reported. PMID: 26035385
    6. Enolase is a new target of liver X receptors. PMID: 25708389
    7. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic PMID: 25643152
    8. MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
    9. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
    10. These results demonstrate the novel requirement of alpha-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
    11. ENO1-positive monocytes and macrophages are found in arthritic ankle tissues of mice with collagen-induced arthritis. PMID: 22623332
    12. By concentrating and enhancing plasmin generation on the cell surface, alpha-enolase-type plasminogen receptor is an important component of skeletal myogenesis. PMID: 14515195
    13. the expression of alpha-enolase plasminogen receptor may serve to concentrate and enhance plasmin generation on the cell surface of migratory myoblasts contributing to efficient muscle repair [review] PMID: 15574344
    14. plasminogen stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at alpha-enolase promoter PMID: 16225843

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  • 亞細胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Enolase family
  • 組織特異性：
    Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma het
  • 數據庫鏈接：


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