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  4. Mouse Albumin (Alb)ELISA Kit

Mouse Albumin (Alb)ELISA Kit

  • 中文名稱：
    小鼠白蛋白酶聯免疫試劑盒
  • 貨號：
    CSB-E13878m
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠白蛋白酶聯免疫試劑盒（CSB-E13878m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ALB含量。ALB即白蛋白，是血漿中含量最豐富的蛋白質。在藥物研究中常作為靶點，其背景在于能與多種內源性和外源性物質結合。研究機制主要圍繞利用其結合特性，開發與之結合的藥物，以實現藥物的運輸、控釋等，提升藥物療效與安全性。試劑盒檢測范圍為0.781 μg/mL-200 μg/mL，靈敏度為0.110 μg/mL。該產品適用于肝臟疾病模型研究、代謝綜合征分析、藥物毒性評估及生物標志物篩選等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Alb 1 ELISA Kit; alb ELISA Kit; ALBU_MOUSE ELISA Kit; Albumin 1 ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.781 μg/mL-200 μg/mL
  • 靈敏度：
    0.110 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess. 

  • 線性度：

     To assess the linearity of the assay, samples were spiked with high concentrations of mouse albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 標準曲線：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with the anti-mouse Alb antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled Alb (100 x concentrate) (60 μl/bottle)
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (20 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute the HRP-avidin solution.
      • Two vial Sample Diluent (20 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：組織勻漿液 大鼠視網膜組織勻漿中的TNF-α

樣品信息：小鼠

稀釋比：其他 1:1000

產品評價： 我是實驗小白，打算做模式動物的研究，當時篩選了好幾個指標，對于這種情況，不太清楚該用什么盒子。因此咨詢了他們技術人員，他們工作人員從反應機理、作用途徑、反饋情況幫我查閱相關資料，相互交流，最終確定了合適的產品。我使用了CSB-E11987r檢測了處理前后大鼠視網膜組織勻漿中TNF-α的水平含量，標曲擬合度良好，操作簡單快捷靈敏度高，能助力我順利完成相關的實驗數據的收集。

By 楊老師

靶點詳情

  • 最新研究進展：
        白蛋白（ALB）是一種常見的血漿蛋白，具有多種生理功能，包括維持血容量、調節血液滲透壓和輸送物質等。以下是關于ALB最新的研究進展：
        COVID-19：研究人員發現COVID-19患者血清中ALB水平下降，與嚴重程度呈正相關。低ALB水平與患者預后不良相關，提示ALB作為COVID-19預后的生物標志物具有潛在價值。
        腎病：ALB是腎病患者中最常見的蛋白質丟失之一。研究表明，ALB水平的下降可能導致腎臟損傷和慢性腎臟病的進展。因此，ALB已成為評估腎臟健康的重要指標之一。
        癌癥：一些研究表明，ALB水平下降與腫瘤發生和惡化有關。低ALB水平與預后不良相關，因此ALB可能是腫瘤預后評估的潛在生物標志物之一。
        營養：研究表明，ALB水平可以用作營養狀況的指標。ALB水平下降通常與營養不良相關，因此，ALB水平可能有助于評估患者的營養狀況。
        總之，最新的研究表明，ALB在許多不同疾病中都發揮著重要的生物學作用，并可能成為評估患者預后和健康狀況的重要指標之一。
  • 功能：
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 基因功能參考文獻：
    1. findings suggest that HMGB1 induces the transcytosis of albumin via RAGE-dependent Src phosphorylation and Cav-1 phosphorylation. These studies revealed a new mechanism of HMGB1-induced endothelial hyperpermeability. PMID: 27572515
    2. Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase of kidney injury and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. These markers could detect higher risk of progression to CKD. PMID: 27028054
    3. Extending serum half-life of albumin by engineering neonatal Fc receptor (FcRn) binding. PMID: 24652290
    4. We provide evidence of a transcytosis within the kidney tubular system that protects albumin and IgG from lysosomal degradation, allowing these proteins to be recycled intact. PMID: 23970123
    5. albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation. PMID: 20529675
    6. No binding of albumin was observed at physiological pH to neonatal Fc receptor. At acidic pH, a 100-fold difference in binding affinity was observed. PMID: 20018855
    7. Cellular oxidant stress and advanced glycation endproducts of albumin: caveats of the dichlorofluorescein assay PMID: 11913966
    8. albumin enhancer/promoter was succesfully used for trasient transfection of fluorescent gene reporters (for cell sorting) in ES spontaneously differentiated into hepatocytes. PMID: 18942772

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 組織特異性：
    Plasma. Expressed in the granular cells within the cerebellum.
  • 數據庫鏈接：


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