在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

  • 中文名稱：
    小鼠丙氨酸轉氨酶/谷丙轉氨酶(ALT/GPT)酶聯免疫試劑盒
  • 貨號：
    CSB-E16539m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠丙氨酸轉氨酶/谷丙轉氨酶(ALT/GPT)酶聯免疫試劑盒（CSB-E16539m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、細胞裂解物樣本中的ALT/GPT含量。ALT/GPT即谷丙轉氨酶，主要存在于肝細胞內。肝細胞受損時其釋放入血，致血清ALT/GPT水平升高，是反映肝細胞損傷的敏感指標。臨床常通過檢測其活性輔助診斷肝臟疾病，如肝炎、肝硬化等，研究也圍繞其在疾病中的變化機制展開。試劑盒檢測范圍為0.156 mU/mL-10 mU/mL，廣泛應用于肝損傷模型評估、藥物肝毒性研究、代謝性疾病機制探索等科研領域，特別適用于體外培養肝細胞或動物模型中肝臟相關病理生理變化的動態監測，為肝臟功能研究提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
  • 縮寫：
    ALT/GPT
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, cell lysates
  • 檢測范圍：
    0.156 mU/mL-10 mU/mL
  • 靈敏度：
    0.039 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %83-95
    1:2Average %97
    Range %92-101
    1:4Average %106
    Range %98-113
    1:8Average %94
    Range %87-97
  • 回收率：
    The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)10295-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    102.645 2.566 2.606 2.421
    52.015 1.855 1.935 1.750
    2.51.194 1.155 1.175 0.990
    1.250.777 0.796 0.787 0.602
    0.6250.492 0.461 0.477 0.292
    0.3120.335 0.344 0.340 0.155
    0.1560.278 0.288 0.283 0.098
    00.191 0.178 0.185 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • 基因功能參考文獻：
    1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
    2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
    3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
  • 組織特異性：
    Mainly expressed in liver, intestine, colon and white adipose tissue.
  • 數據庫鏈接：


主站蜘蛛池模板： 亚洲成av人在线视| 欧美精品v国产精品v日韩精品 | 国产亚洲产品影市在线产品| 四虎www成人影院观看| 18禁免费无码无遮挡不卡网站| 日本乱人伦在线观看| 日产国产精品亚洲系列| 国产丰满麻豆vⅰde0sex| 久九九久视频精品免费| 丰满少妇大力进入| 特大巨黑吊xxxx高潮| 亚欧洲精品在线视频免费观看| 日日av色欲香天天综合网| 亚洲一卡2卡三卡4卡无卡下载 | 丰满熟女人妻一区二区三| 蜜桃无码一区二区三区| 狼群社区www中文视频| 内谢少妇xxxxx8老少交| 射精区-区区三区| 人妻精品无码一区二区三区| 国产 精品 自在 线免费| 国产午夜无码片在线观看影视| 亚洲老熟女性亚洲| 夜夜影院未满十八勿进| 国产成人精品.视频| 国产av毛片亚洲含羞草社| 久久久久人妻一区精品| 亚洲a∨无码无在线观看| 亚洲国产一区二区波多野结衣| 欧美乱妇xxxxxbbbbb| 大屁股熟女一区二区三区| 99久久无码一区人妻| 一本久久a久久免费精品不卡| 国产亚洲日韩欧美另类丝瓜app | 亚洲中文字幕一区精品自拍| 国产男女免费完整视频| 国产在线观看免费观看不卡| 久久996re热这里有精品| 人妻熟女一区| 欧美xxxxx高潮喷水麻豆| 韩国无码色视频在线观看|