Mouse Activin A,ACV-A ELISA Kit
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中文名稱:小鼠活化素A(ACV-A)酶聯免疫試劑盒
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貨號:CSB-E04502m
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規格:96T/48T
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價格:¥3800/¥2500
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其他:
產品詳情
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產品描述:小鼠活化素A(ACV-A)酶聯免疫試劑盒(CSB-E04502m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ACV-A含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,靈敏度為3.9 pg/mL。適用于小鼠模型在基礎研究中的多場景應用,例如探究生殖生物學機制、炎癥性疾病模型中的分子調控、組織再生研究或藥物干預下ACV - A的動態變化分析,助力于發育生物學、免疫學及病理機制等領域的深入探索。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:ACV-A
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
122.000
132.198
122.651
123.043
120.448
120.448
SD
0.055
0.064
0.063
0.071
0.068
0.074
CV(%)
5.56
6.01
6.34
7.12
6.96
7.58
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
86-105
1:2
Average %
96
Range %
91-100
1:4
Average %
93
Range %
90-99
1:8
Average %
96
Range %
89-100
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回收率:
The recovery of mouse ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-99
EDTA plasma (n=4)
97
92-100
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.249
2.268
2.259
2.175
500
2.035
2.055
2.045
1.961
250
1.601
1.586
1.594
1.510
125
0.954
0.972
0.963
0.879
62.5
0.534
0.521
0.528
0.444
31.2
0.272
0.283
0.278
0.194
15.6
0.184
0.190
0.187
0.103
0
0.083
0.085
0.084
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數據處理:
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貨期:3-5 working days
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