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  4. Monkey Tumor necrosis factor α,TNF-α ELISA KIT

Monkey Tumor necrosis factor α,TNF-α ELISA KIT

  • 中文名稱：
    猴腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E10039Mo
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    猴腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E10039Mo）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNF含量。TNF即腫瘤壞死因子，是一種多功能細(xì)胞因子。在背景上，它最初因能使腫瘤組織出血壞死而被發(fā)現(xiàn)。其研究機(jī)制主要涉及參與免疫調(diào)節(jié)、炎癥反應(yīng)等過程。異常的TNF表達(dá)與多種疾病相關(guān)，如類風(fēng)濕關(guān)節(jié)炎等，是相關(guān)疾病治療的重要靶點(diǎn)。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，該靶標(biāo)常用于靈長類動(dòng)物感染性疾病、自身免疫模型及炎癥機(jī)制研究；適用于恒河猴、食蟹猴等非人靈長類動(dòng)物的基礎(chǔ)研究，如病毒感染模型中細(xì)胞因子風(fēng)暴評(píng)估、免疫抑制劑作用機(jī)制探索，或通過組織樣本分析特定器官炎癥微環(huán)境變化，為神經(jīng)退行性疾病、代謝綜合征等科研方向提供關(guān)鍵數(shù)據(jù)支持；適用于實(shí)驗(yàn)室常規(guī)蛋白定量分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Monkey
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.105 2.097 2.101 2.008
    501.863 1.805 1.834 1.741
    251.412 1.395 1.404 1.311
    12.50.909 0.896 0.903 0.810
    6.250.594 0.549 0.572 0.479
    3.120.306 0.298 0.302 0.209
    1.560.191 0.187 0.189 0.096
    00.096 0.090 0.093 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：其他 猴

稀釋比：其他 1：3，1;5，1:10

產(chǎn)品評(píng)價(jià)： 我用CSB-E10039Mo檢測(cè)食蟹猴細(xì)胞培養(yǎng)上清液，標(biāo)曲OD值范圍為3.1274-0.2045，樣品檢測(cè)OD值范圍為1.112-0.109，檢測(cè)良好，標(biāo)曲正常。

By 榮老師

靶點(diǎn)詳情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能參考文獻(xiàn)：
    1. These interactions result in the induction of the TNF signaling pathway, activation of apoptosis, and DNA-damage stress response. PMID: 24743303
    2. Findings showed that both mucosal compartments harbor similar percentages of memory CD4(+) T cells and displayed comparable cytokine TNF-alpha responses to mitogenic stimulations prior to infection. PMID: 24610016
    3. Primary role for IL-1beta and TNF-alpha in the triggering of preterm labor associated with inflammation or infection. PMID: 17132473
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫鏈接：


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