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Monkey Interferon γ,IFN-γ ELISA Kit

  • 中文名稱:
    猴γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號:
    CSB-E10049Mo
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    猴γ干擾素(IFN-γ)酶聯免疫試劑盒(CSB-E10049Mo)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ,在免疫調節等方面背景重要。它是由活化的T細胞和NK細胞產生的細胞因子。研究機制上,可激活巨噬細胞、增強MHC分子表達、調節Th1/Th2平衡等,在機體免疫防御、抗腫瘤等過程中發揮關鍵作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,支持科研人員開展非人靈長類動物模型的免疫機制研究、感染性疾病模型評估或藥物干預實驗中的細胞因子動態監測本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Monkey
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20001.830 1.990 1.910 1.805
    10001.496 1.398 1.447 1.342
    5001.133 1.138 1.136 1.031
    2500.932 0.902 0.917 0.812
    1250.544 0.535 0.540 0.435
    62.50.212 0.226 0.219 0.114
    31.250.117 0.111 0.114 0.009
    00.106 0.104 0.105  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:細胞培養上清液

樣品信息:其他 猴

稀釋比:其他 1:3,1:5

產品評價: 我用CSB-E10049Mo檢測食蟹猴細胞培養上清液,標曲OD值范圍為2.780-0.1055,樣品檢測OD值范圍為0.1274-0.0503,檢測結果低于下限,可能是稀釋倍數問題,標曲正常。

By 榮老師

靶點詳情

  • 功能:
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻:
    1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
    2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
    3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
    4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Type II (or gamma) interferon family
  • 組織特異性:
    Released primarily from activated T lymphocytes.
  • 數據庫鏈接:


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