Monkey Brain-derived neurotrophic factor(BDNF) ELISA kit

說明書下載

中文名稱：

猴腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL002655RH
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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猴腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)酶聯(lián)免疫試劑盒（CSB-EL002655RH）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的BDNF含量。BDNF即腦源性神經(jīng)營(yíng)養(yǎng)因子，是神經(jīng)營(yíng)養(yǎng)因子家族重要成員。它在神經(jīng)系統(tǒng)的發(fā)育、維持神經(jīng)元存活和分化等方面發(fā)揮關(guān)鍵作用。其研究機(jī)制主要圍繞與TrkB受體結(jié)合，激活下游信號(hào)通路，對(duì)神經(jīng)可塑性、學(xué)習(xí)記憶等產(chǎn)生影響。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，本試劑盒通過高特異性抗體對(duì)目標(biāo)蛋白進(jìn)行捕獲與檢測(cè)，操作流程標(biāo)準(zhǔn)化，可穩(wěn)定分析不同來源樣本的BDNF濃度差異，為神經(jīng)生物學(xué)基礎(chǔ)研究、疾病動(dòng)物模型評(píng)估或藥物干預(yù)效果驗(yàn)證等科研場(chǎng)景提供可靠數(shù)據(jù)支持。BDNF表達(dá)水平變化常被用于神經(jīng)退行性疾病、抑郁癥及認(rèn)知功能相關(guān)機(jī)制的科學(xué)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit; Fragment ELISA kit
縮寫：

BDNF
Uniprot No.：

Q06225
種屬：

Monkey
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.391	2.219	2.305	2.195
25	1.791	1.812	1.802	1.692
12.5	1.320	1.366	1.343	1.233
6.25	0.801	0.845	0.823	0.713
3.12	0.557	0.553	0.555	0.445
1.56	0.369	0.388	0.379	0.269
0.78	0.254	0.265	0.260	0.150
0	0.107	0.112	0.110

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
亞細(xì)胞定位：

Secreted.; [BDNF precursor form]: Secreted.
蛋白家族：

NGF-beta family
數(shù)據(jù)庫(kù)鏈接：

STRING: 9544.ENSMMUP00000011324

UniGene: Mmu.3380

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Monkey Brain-derived neurotrophic factor(BDNF) ELISA kit

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