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中文名稱:人α-黑色素細胞刺激素(α-MSH)酶聯免疫試劑盒
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貨號:CSB-E09658h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人α-黑色素細胞刺激素(α-MSH)酶聯免疫試劑盒(CSB-E09658h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的α-MSH含量。α - MSH即α - 促黑素細胞激素,是一種內源性神經肽。它在調節皮膚色素沉著、能量代謝、炎癥反應等方面發揮重要作用。研究機制主要涉及與黑素皮質素受體結合,激活下游信號通路,進而對多種生理功能產生影響。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,該產品廣泛應用于基礎科研領域,如探究α-MSH在代謝綜合征動物模型中的表達特征、評估體外細胞刺激實驗的激素分泌水平,或解析皮膚黑色素生成調控機制等研究場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:alpha-melanocyte stimulating hormone, alpha-MSH
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縮寫:α-MSH
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Biochemicals
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 92-104 1:2 Average % 95 Range % 91-104 1:4 Average % 89 Range % 85-93 1:8 Average % 94 Range % 86-98 -
回收率:
The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 89-95 EDTA plasma (n=4) 94 89-97 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.081 2.181 2.131 2.000 5 1.256 1.198 1.227 1.096 2.5 0.655 0.635 0.645 0.514 1.25 0.406 0.416 0.411 0.280 0.625 0.260 0.270 0.265 0.134 0.312 0.220 0.219 0.220 0.089 0.156 0.187 0.182 0.185 0.054 0 0.132 0.130 0.131 -
數據處理:
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貨期:3-5 working days
引用文獻
- Serum melanin and alpha-melanocyte stimulating hormone levels in Pakistani individuals affected with oculocutaneous albinism M Shakil,Rawal Medical Journal,2023
- Particulate matter promotes hyperpigmentation via AhR/MAPK signaling activation and by increasing α-MSH paracrine levels in keratinocytes Rong Xiao,Environmental Pollution,2021
- Efficacy of a wound-dressing biomaterial on prevention of postinflammatory hyperpigmentation after suction blister epidermal grafting in stable vitiligo patients: a … Z Liu,Archives of Dermatological Research,2020
- A ROCK inhibitor promotes keratinocyte survival and paracrine secretion, enhancing establishment of primary human melanocytes and melanocyte-keratinocyte co-cultures Mi J, et al,Pigment Cell & Melanoma Research,2019
- Dietary Supplementation with Raspberry Seed Oil Modulates Liver Functions, Inflammatory State, and Lipid Metabolism in Rats Fotschki B. et al,J Nutr,2015
產品評價
樣品類型:細胞培養上清液
樣品信息:人
稀釋比:沒有稀釋
產品評價: 我用的是人源α-MSH試劑盒。本次實驗目的:探究UVB對角質細胞分泌α-MSH的影響及多肽樣品的作用。實驗樣品:UVB照射后,細胞培養箱中孵育24h,收取細胞分泌上清液。實驗組OD值是0.15-0.31。以上為近期所用后的反饋,請查收!
By 雷老師
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