Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit
-
中文名稱:人Ⅱ型膠原螺旋肽(HELIX-Ⅱ)酶聯(lián)免疫試劑盒
-
貨號:CSB-E09708h
-
規(guī)格:96T/48T
-
價格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:人Ⅱ型膠原螺旋肽(HELIX-Ⅱ)酶聯(lián)免疫試劑盒(CSB-E09708h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、尿液樣本中的HELIXⅡ含量。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL,Ⅱ型膠原是關節(jié)軟骨的主要結構蛋白,其螺旋結構域(HELIX - Ⅱ)的降解產(chǎn)物可作為軟骨代謝異常的分子標志物,在骨關節(jié)疾病機制研究、軟骨損傷動態(tài)監(jiān)測及藥物干預效果評價等領域具有重要科研價值。研究人員可通過該產(chǎn)品探究骨關節(jié)炎、類風濕性關節(jié)炎等疾病進程中軟骨基質降解的動態(tài)變化,或用于篩選調節(jié)軟骨代謝的潛在化合物,為相關領域的分子機制研究和治療策略開發(fā)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名:type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
-
縮寫:HELIXⅡ
-
種屬:Homo sapiens (Human)
-
樣本類型:serum, plasma, urine
-
檢測范圍:1.25 ng/mL-80 ng/mL
-
靈敏度:0.312 ng/mL
-
反應時間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領域:Others
-
測定原理:quantitative
-
測定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 89 Range % 83-94 1:4 Average % 99 Range % 94-103 1:8 Average % 104 Range % 100-112 -
回收率:
The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 80-88 EDTA plasma (n=4) 91 86-97 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 80 2.178 2.114 2.146 2.043 40 1.674 1.581 1.628 1.525 20 1.116 1.061 1.089 0.986 10 0.626 0.603 0.615 0.512 5 0.423 0.407 0.415 0.312 2.5 0.252 0.239 0.246 0.143 1.25 0.193 0.184 0.189 0.086 0 0.104 0.101 0.103 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-