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Human tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA Kit

  • 中文名稱:
    人基質金屬蛋白酶抑制因子3(TIMP-3)酶聯免疫試劑盒
  • 貨號:
    CSB-E04734h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人基質金屬蛋白酶抑制因子3(TIMP-3)酶聯免疫試劑盒(CSB-E04734h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TIMP3含量。TIMP3 即金屬蛋白酶組織抑制因子 3,是內源性 MMP 抑制劑。它能與 MMPs 活性中心的鋅離子結合,抑制 MMPs 活性。其在腫瘤、心血管疾病等多種疾病中表達異常,通過調節細胞外基質代謝、抑制血管生成等機制參與疾病發生發展。試劑盒檢測范圍為31.25 pg/ml-2000 pg/ml,靈敏度為7.8 pg/ml。為研究TIMP - 3在疾病模型中的表達調控、藥物干預效果評估以及分子機制探索提供可靠工具,廣泛應用于細胞生物學、病理學及藥理學等科研領域,尤其適用于涉及組織損傷修復、腫瘤微環境或代謝性疾病的基礎研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    HSMRK222 ELISA Kit; K222 ELISA Kit; K222TA2 ELISA Kit; Metalloproteinase inhibitor 3 ELISA Kit; MIG 5 protein ELISA Kit; MIG5 protein ELISA Kit; Protein MIG 5 ELISA Kit; Protein MIG-5 ELISA Kit; SFD ELISA Kit; Sorsby fundus dystrophy pseudoinflammatory ELISA Kit; TIMP 3 ELISA Kit; TIMP metallopeptidase inhibitor 3 ELISA Kit; TIMP-3 ELISA Kit; TIMP3 ELISA Kit; TIMP3_HUMAN ELISA Kit; Tissue Inhibitor of Metalloproteinase 3 ELISA Kit; Tissue inhibitor of metalloproteinases 3 ELISA Kit; Tissue inhibitor of metalloproteinases3 ELISA Kit
  • 縮寫:
    TIMP3
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/ml-2000 pg/ml
  • 靈敏度:
    7.8 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    246.660

    246.505

    242.560

    248.749

    245.363

    251.011

    SD

    0.038

    0.035

    0.046

    0.034

    0.033

    0.031

    CV(%)

    6.221

    5.710

    7.590

    5.574

    5.464

    5.049

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:4

    Average %

    91

    Range %

    87-98

    1:8

    Average %

    90

    Range %

    85-94

    1:16

    Average %

    92

    Range %

    88-93

    1:32

    Average %

    96

    Range %

    89-98

  • 回收率:

    The recovery of human TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    91

    87-94

    EDTA plasma (n=4)

    95

    88-97

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.753

    3.081

    2.917

    2.761

    1000

    1.636

    1.796

    1.716

    1.560

    500

    1.030

    1.088

    1.059

    0.903

    250

    0.541

    0.565

    0.553

    0.397

    125

    0.390

    0.365

    0.378

    0.222

    62.5

    0.261

    0.247

    0.254

    0.098

    31.25

    0.202

    0.215

    0.209

    0.053

    0

    0.154

    0.157

    0.156

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    TIMP3是TIMP家族中的另一成員,與細胞增殖和基質沉積等方面有關。研究表明,TIMP3參與了多種疾病的發生和發展,如心血管疾病、腫瘤和神經系統疾病等。最近的研究還發現,TIMP3可能參與了人類的老化和炎癥反應,成為潛在的治療靶點。
  • 功能:
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
  • 基因功能參考文獻:
    1. Genetic variation in the TIMP-3 gene may contribute to individual differences in mixture plaque susceptibility in the Han Chinese population. PMID: 29498555
    2. TIMP3 methylation is a marker for TN tumours and furthermore we showed for the first time that TIMP3 promoter methylation is an epigenetic marker of BRCA1ness tumours. PMID: 29524167
    3. As a novel CLOCK-dependent diurnal gene, TIMP3 inhibits the expression of inflammatory cytokines that are up-regulated by UV irradiation in human keratinocytes. PMID: 29180440
    4. miR-21-5p mediates apoptosis by targeting PTEN and TIMP3. PMID: 29393355
    5. Preliminary studies indicate that baseline MMP3 and TIMP3 concentrations are associated with patient survival and disease-free time PMID: 29304854
    6. TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ breast carcinomas and negatively in progesterone receptor positive invasive breast carcinomas. PMID: 28935174
    7. Sphingosine-1-phosphate inhibited cell migration and MMP-2 expression through the upregulation of the tissue inhibitor of metalloproteinase-3 (TIMP-3) expression in human chondrosarcoma cells. PMID: 28672103
    8. Using global proteomic profiling of brain leptomeningeal arteries, this study revealed that clusterin and tissue inhibitor of metalloproteinases-3 increase in leptomeningeal arteries affected by cerebral amyloid angiopathy. PMID: 27543695
    9. This is the first report of a syndromic Sorsby fundus dystrophy in line with the mouse model uncovering the role of TIMP3 in human lung morphogenesis and functions. PMID: 27601084
    10. Collectively, these results demonstrated that IL-32alpha upregulates the atheroprotective genes Timp3 and Reck by downregulating microRNA-205 through regulation of the Rprd2-Dgcr8/Ddx5-Dicer1 biogenesis pathway. PMID: 28740544
    11. MMP-13 may play a role on physiological turnover of cartilage extracellular matrix and that LRP1 is a key modulator of extracellular levels of MMP-13 and its internalization is independent of the levels of ADAMTS-4, -5 and TIMP-3. PMID: 27084377
    12. we show that KDM1A promotes cancer metastasis in non-small cell lung cancer cells by repressing TIMP3 (tissue inhibitor of metalloproteinase 3) expression. PMID: 27058897
    13. Dissecting the interaction between TIMP3 and LRP1 using a synthetic analog of the LRP1 receptor has been reported. PMID: 27476612
    14. These results implicate TIMP3 as a modulator of cell surface GHR abundance and the ability of GH to promote cellular signaling. PMID: 27075707
    15. native glycosaminoglycans interact with TIMP-3. PMID: 27545813
    16. The expression level of LIPC, SLC16A8, and TIMP-3 was significantly associated with age-related macular degeneration pathology. PMID: 27966779
    17. Levels of miR-221/222 are associated negatively with estrogen receptor in in situ tumors and positively with tissue inhibitor of metalloproteinase 3 TIMP3 messenger RNA expression levels in pure invasive breast cancers. PMID: 27488105
    18. Electrostatic potential calculations suggested a competition between negatively charged GAGs and highly negatively charged complement-like domains of LRP-1 for the binding to a positively charged area of TIMP-3 as an underlying mechanism. PMID: 27610455
    19. TIMP3 overexpression after myocardial infarction improves myocardial structural remodeling and function by promoting angiogenesis and inhibiting early proteolysis. PMID: 28550172
    20. Single Nucleotide Variants of Candidate Genes in Aggrecan Metabolic Pathway Are Associated with Lumbar Disc Degeneration and Modic Changes PMID: 28081267
    21. Our data suggest that miR-206 may function as an inflammatory regulator and drive the expression of MMP9 in M.tb-infected THP-1 cells by targeting TIMP3, indicating that miR-206 is a potential therapeutic target for patients with TB. PMID: 27291149
    22. Plasma TIMP3 is a biomarker for predicting the tumor stage in patients with oral squamous cell carcinoma . PMID: 28138307
    23. TIMP-3 K26A/K45A retained higher affinity for sulfated glycosaminoglycans than K42A/K110A and exhibited increased affinity for ADAMTS-5 in the presence of heparin. PMID: 27582494
    24. Of the 225 genetic tests performed, 150 were for recessive IRD, and 75 were for dominant IRD. A positive molecular diagnosis was made in 70 (59%) of probands with recessive IRD and 19 (26%) probands with dominant IRD. Thirty-two novel variants were identified; among these, 17 sequence changes in four genes were predicted to be possibly or probably damaging including: ABCA4 (14), BEST1 (2), PRPH2 (1), and TIMP3 PMID: 28005406
    25. Study evaluated MMP-12 and TIMP-1, TIMP-2, TIMP-3, and TIMP-4 levels in 40 patients with asymptomatic and symptomatic critical carotid artery stenosis (CAS) with neurologic symptoms onset within the preceding 12 hours; results suggest that MMP-12 is related to critical CAS independently on symptoms, moreover, TIMP-3 and TIMP-4 seem to be specifically related to stroke PMID: 27746079
    26. A total of 1096 subjects from eight studies were included in the present meta-analysis. Overall, a significant association between TIMP-3 methylation and gastric cancer risk was observed (OR = 8.65; 95% CI4.31-17.37; p < 0.001). Our results show a positive correlation between TIMP-3 promoter methylation and gastric cancer risk and that TIMP-3 promoter methylation may be used as a molecular marker for gastric cancer PMID: 27314831
    27. TIMP-3 expression is suppressed by promoter methylation in HCC. PMID: 27222429
    28. Both TIMP3 and APC methylation were associated with lymph node metastasis and higher clinical stage of tumors. Patients with methylation at TIMP3 or APC had worse prognoses as compared to those without these alterations. PMID: 27706614
    29. Data indicate the TGF-beta pathway regulates the epithelial-to-mesenchymal transition (EMT) of gastric cancer cells by increasing the levels of microRNA miRNA-181b to target Timp3 via the Smad2/3/4-dependent pathway. PMID: 27383203
    30. Study identifies 2 urinary biomarkers-bFGF and TIMP3-that successfully detect one of the most common pediatric brain tumors, juvenile pilocytic astrocytomas, with high accuracy PMID: 27314542
    31. the levels of TIMP-3, and in some cases also TIMP-2, are decreased in Emery-Dreifuss muscular dystrophy (EDMD). The decrease might be associated with an adverse effect on matrix metalloproteinases and remodelling of the myocardial matrix. The specific decrease of TIMP-3 indicates that this biomarker might help in early detection of cardiac involvement in EDMD. PMID: 25563468
    32. TIMP3 knockdown had opposite effects on the regulation of these genes. PMID: 26749283
    33. This case series suggests the C113G TIMP3 variant may represent a novel highly penetrant mutation causing choroidal neovascularisation of relatively late onset for Sorsby's fundus dystrophy, mimicking early onset AMD. PMID: 26493035
    34. expression of TIMP3 is low in pituitary adenomas including ACTH-secreting pituitary adenomas and negatively associated with tumor aggressiveness. PMID: 26676407
    35. Genotypes of rs135025 and rs80272 in TIMP3 contribute to the development of preeclampsia in Han Chinese women. PMID: 26304100
    36. TIMP3 was validated as a direct target of miRNA-21 by dual-luciferase reporter assay. Silencing with small interfering RNA against TIMP3 promoted angiogenesis and increased MMP2 and MMP9 expression at the protein level. PMID: 26872030
    37. TIMP3 is a dominant negative regulator of angiogenesis in cutaneous melanoma and gene silencing by promoter methylation is associated with poor outcome PMID: 26707830
    38. Taken together, our results suggest that the imbalance between aggrecanase and TIMP-3 may play an important role in the pathogenesis of IDD and therefore be a potential therapeutic target for treating IDD. PMID: 26686417
    39. Gene-gene interactions between Smad3 rs6494629T/C and TIMP3 rs715572G/A polymorphisms may play more important protective roles in knee OA. PMID: 26068512
    40. Results suggest that gene-environment interactions between the TIMP3 rs9862 polymorphisms and betel quid may alter oral cancer susceptibility and tumor growth in Taiwanese men. PMID: 26579821
    41. following acute ACL injury, an upregulation of TIMP-3, the primary aggrecanase inhibitor, is elicited in response to increased aggrecan degradation, which may inhibit further cleavage PMID: 26123869
    42. TIMP3) promotes endothelial apoptosis via a caspase-independent mechanism. PMID: 25558000
    43. This data shows that increased expression of miR-21 enhanced the invasive potential of melanoma cell lines through TIMP3 inhibition. PMID: 25587717
    44. The miR-191-TIMP3 axis might be critical in the malignant transformation of endometriosis to endometriosis-associated ovarian cancer. PMID: 25819812
    45. TIMP-3 expression was associated with malignant behaviors of hepatocellular carcinoma, including portal vein invasion and lymph node metastasis. TIMP-3 expression was an independent prognostic factor for disease-free survival and overall survival PMID: 25171061
    46. TIMP3 methylation had prognostic value in patients with glioblastoma multiforme. PMID: 25467143
    47. This study expands the spectrum of mutations in the TIMP3 gene and associated phenotypic findings. Imaging using late-phase ICG-A may be useful for early identification of individuals at risk for developing SFD. PMID: 25766588
    48. The hypermethylation frequencies of TIMP-3 and GSTP-1 of reversible chronic inflammatory gum disease and the control group were similar, but both were significantly lower than those for malignant disease patients. PMID: 25041782
    49. HPV-positive oropharyngeal squamous cell carcinoma is associated with TIMP3 and CADM1 promoter hypermethylation. PMID: 25065733
    50. Report no association of TIMP3 genetic polymorphisms with thoracic aortic dissection in Chinese Han population. PMID: 24487965

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  • 相關疾?。?/div>
    Sorsby fundus dystrophy (SFD)
  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Protease inhibitor I35 (TIMP) family
  • 數據庫鏈接:

    HGNC: 11822

    OMIM: 136900

    KEGG: hsa:7078

    STRING: 9606.ENSP00000266085

    UniGene: Hs.644633



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