Human prothrombin fragment 1+2,F1+2 ELISA kit
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中文名稱:人凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09822h
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒(CSB-E09822h)檢測(cè)血清、血漿、組織勻漿樣本中的F1+2含量。人凝血酶原片段F1+2是凝血酶原激活轉(zhuǎn)化為凝血酶過程中釋放的穩(wěn)定降解產(chǎn)物,其含量反映凝血酶早期激活水平,可用于診斷血栓前狀態(tài)和血栓性疾病。研究顯示,F(xiàn)1+2在凝血途徑中起關(guān)鍵作用,其檢測(cè)有助于疾病診斷和治療監(jiān)測(cè)。試劑盒檢測(cè)范圍為0.03 nmol/L -2 nmol/L,該產(chǎn)品廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究中凝血/纖溶系統(tǒng)動(dòng)態(tài)評(píng)估、藥物抗凝效果評(píng)價(jià)、動(dòng)物模型血栓形成機(jī)制探索等科研領(lǐng)域,為心血管疾病、腫瘤轉(zhuǎn)移等病理過程的分子機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:F1+2
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.03 nmol/L -2 nmol/L
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靈敏度:0.007 nmol/L
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
89-99
1:2
Average %
98
Range %
93-102
1:4
Average %
86
Range %
81-93
1:8
Average %
92
Range %
88-97
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回收率:
The recovery of human F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
90-96
EDTA plasma (n=4)
87
83-90
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/L
OD1
OD2
Average
Corrected
2
2.296
2.274
2.285
2.137
1
1.445
1.498
1.472
1.324
0.5
0.916
0.936
0.926
0.778
0.25
0.538
0.568
0.553
0.405
0.125
0.376
0.398
0.387
0.239
0.06
0.270
0.291
0.281
0.133
0.03
0.218
0.216
0.217
0.069
0
0.146
0.149
0.148
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- PAD4-Induced NETosis Via Cathepsin G–Mediated Platelet-Neutrophil Interaction in ChAdOx1 Vaccine-Induced Thrombosis R Carnevale,Arteriosclerosis, thrombosis, and vascular biology,2023
- Glycocalyx┸\Like Hydrogel Coatings for Small Diameter Vascular Grafts S Dimitrievska,Advanced Functional Materials,2020
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