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Human proteoglycan 4(PRG4) ELISA Kit

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產品描述：

人蛋白聚糖4(PRG4)酶聯免疫試劑盒（CSB-E14124h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的PRG4含量。試劑盒檢測范圍為0.78 ng/ml-50 ng/ml，靈敏度為0.195 ng/ml。該產品適用于關節炎機制研究、藥物開發中生物標志物分析，或體外模型中 PRG4 表達水平的動態監測，為探索關節退行性疾病病理機制及潛在治療靶點提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

PRG4 ELISA Kit; MSF ELISA Kit; SZP ELISA Kit; Proteoglycan 4 ELISA Kit; Lubricin ELISA Kit; Megakaryocyte-stimulating factor ELISA Kit; Superficial zone proteoglycan) [Cleaved into: Proteoglycan 4 C-terminal part] ELISA Kit

縮寫：

PRG4

Uniprot No.：

Q92954

種屬：

Homo sapiens (Human)

樣本類型：

serum, plasma and tissue homogenates.

檢測范圍：

0.78 ng/ml-50 ng/ml.

靈敏度：

0.195 ng/ml

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Others

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	109
	Range %	102-115
1:2	Average %	100
	Range %	95-106
1:4	Average %	94
	Range %	89-100
1:8	Average %	101
	Range %	96-107

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

50

2.524

2.634

2.579

2.406

25

1.902

1.967

1.934

1.762

12.5

1.286

1.228

1.257

1.084

6.25

0.840

0.829

0.835

0.662

3.12

0.476

0.451

0.464

0.291

1.56

0.385

0.373

0.379

0.206

0.78

0.264

0.274

0.269

0.096

0

0.170

0.175

0.173

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PRG4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PRG4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.