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  4. Human protein disulfide isomerase,PDI ELISA Kit

Human protein disulfide isomerase,PDI ELISA Kit

  • 中文名稱：
    人蛋白質二硫鍵異構酶(PDI)酶聯免疫試劑盒
  • 貨號：
    CSB-E09003h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人蛋白質二硫鍵異構酶(PDI)酶聯免疫試劑盒（CSB-E09003h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的P4HB含量。P4HB是一種重要的靶點。它在細胞內參與蛋白質的折疊和穩定，尤其在膠原蛋白合成過程中發揮關鍵作用。相關研究聚焦于其作用機制，探索其與疾病發生發展的聯系，期望通過調控P4HB來開發針對相關疾病的治療方法。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品適用于體外研究PDI在細胞應激調控、蛋白質穩態失衡相關疾病機制中的表達變化，或用于篩選調節PDI活性的化合物，為分子生物學、藥理學及病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Cellular thyroid hormone binding protein ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Collagen prolyl 4 hydroxylase beta ELISA Kit; Disulphide Isomerase ELISA Kit; DSI ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERBA2L ELISA Kit; ERp59 ELISA Kit; GIT ELISA Kit; Gltathione insulin transhydrogenase ELISA Kit; Glutathione insulin transhydrogenase ELISA Kit; P4HB ELISA Kit; P4Hbeta ELISA Kit; p55 ELISA Kit; PDI ELISA Kit; PDIA1 ELISA Kit; PDIA1_HUMAN ELISA Kit; PDIR ELISA Kit; PHDB ELISA Kit; PO4DB ELISA Kit; PO4HB ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit ELISA Kit; PROHB ELISA Kit; Prolyl 4 hydroxylase beta polypeptide ELISA Kit; Prolyl 4 hydroxylase beta subunit ELISA Kit; Prolyl 4 hydroxylase subunit beta ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; Protein disulfide isomerase associated 1 ELISA Kit; Protein disulfide isomerase, family A, member 1 ELISA Kit; Protein disulfide isomerase/oxidoreductase ELISA Kit; Protein disulfide-isomerase ELISA Kit; Protocollagen hydroxylase ELISA Kit; Thbp ELISA Kit; Thyroid hormone binding protein p55 ELISA Kit; Thyroid hormone binding protein p55 cellular ELISA Kit; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %95
    Range %89-99
    1:10Average %98
    Range %93-103
    1:20Average %92
    Range %88-96
    1:40Average %95
    Range %87-99
  • 回收率：
    The recovery of human PDI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9794-101
    EDTA plasma (n=4)8882-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.724 2.587 2.656 2.518
    102.125 2.008 2.067 1.929
    51.368 1.397 1.383 1.245
    2.50.842 0.831 0.837 0.699
    1.250.526 0.538 0.532 0.394
    0.6250.345 0.334 0.340 0.202
    0.3120.262 0.254 0.258 0.120
    00.139 0.137 0.138
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
  • 基因功能參考文獻：
    1. Peroxynitrite preferentially oxidizes the dithiol redox motifs of protein-disulfide isomerase. PMID: 29191937
    2. Overexpression of HIF-1alpha and P4HB is associated with poor prognosis in patients with gastric cancer. PMID: 29904245
    3. Study demonstrated that the expression of P4HB is frequently upregulated at the mRNA and protein levels in diffuse gliomas. Its high expression was significantly correlated with high Ki-67, more TP53 mutations and poor survival outcome. These findings imply that high expression of P4HB plays an important role in diffuse glioma progression. PMID: 29207176
    4. identify a potent and selective PDIA1 inhibitor, KSC-34, with 30-fold selectivity for the a site over the a' site. KSC-34 displays time-dependent inhibition of PDIA1 reductase activity in vitro with a kinact/ KI of 9.66 x 10(3) M(-1) s(-1) and is selective for PDIA1 over other members of the PDI family, and other cellular cysteine-containing proteins. PMID: 29521097
    5. P4HB promotes hepatocellular carcinoma progression by down-regulating GRP78 expression and subsequently promoting epithelial-to-mesenchymal transition. PMID: 28052026
    6. analysis of antiplatelet activity of CxxC through binding to Cys400 in the PDI a0 domain, which can be further exploited as a model for sitedriven antithrombotic agent development PMID: 28109047
    7. Current findings indicate that thiol isomerase-mediated disulfide bond modification in receptors and plasma proteins is an important layer of control of thrombosis and vascular function more generally. PMID: 28598864
    8. DIA1 was robustly secreted by physiological levels of arterial laminar shear in endothelial cells and supported alpha 5 integrin thiol oxidation. PMID: 28034831
    9. Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates. PMID: 28364042
    10. a mechanism of dual Ero1alpha regulation by dynamic redox interactions between PDI and the two Ero1alpha flexible loops that harbor the regulatory cysteines. PMID: 27703014
    11. analysis of how redox affects human protein disulfide isomerase regulate binding affinity of 17 beta-estradiol PMID: 28257787
    12. These findings improve our understanding of PDI-protected aggregation of wild-type alpha-Syn and its H50Q familial mutant. PMID: 27142583
    13. Association of P4HB polymorphisms with sporadic amyotrophic lateral sclerosis susceptibility in the Chinese Han population. PMID: 26000911
    14. the effect of the endoplasmic reticulum chaperone protein disulfide isomerase (PDI) on beta-cell dysfunction, was examined. PMID: 26607804
    15. Amyotrophic lateral sclerosis-linked PDIA1 mutations disrupt motor neuron connectivity. PMID: 26869642
    16. direct binding of PDIA1 to VWF, is reported. PMID: 26670633
    17. Selective sequestration of PDI1A in a calcium depletion-mediated complex with the abundant chaperone calreticulin attenuates the effective concentration of this major lumenal thiol oxidant. PMID: 25575667
    18. Cole-Carpenter syndrome is caused by a specific de novo mutation in P4HB that impairs the disulfide isomerase activity of protein disulfide isomerase. PMID: 25683117
    19. PDI has a role as a competent regulator and a specific substrate of Ero1alpha govern efficient and faithful oxidative protein folding and maintain the ER redox homeostasis PMID: 25258311
    20. The crystal structure of the dimeric form of noncatalytic bb' domains of human PDIA1 determined to 2.3 A resolution revealed that the formation of dimers occludes the substrate binding site. PMID: 24549644
    21. Elevated P4HB expression is associated with temozolomide resistance in malignant glioma. PMID: 23444257
    22. ). The results suggest that P4HB is a modifier gene inamyotrophic lateral sclerosis susceptibility and may represent a potential therapeutic target for amyotrophic lateral sclerosis . PMID: 23337974
    23. A mixed disulfide complex was formed with the catalytic domain A1 from human PDI consistent with a model for cotranslational oxidative protein folding wherein PDI acts as a placeholder that is relieved by the pairing of cysteines caused by substrate folding. PMID: 23141538
    24. TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy PMID: 21373848
    25. these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467
    26. Functional PDI is rapidly secreted from human umbilical vein endothelial cells in culture upon activation with thrombin or after laser-induced stimulation. PMID: 20668226
    27. domain c is required for the stabilization and maintenance of the chaperone function of PDI under extreme conditions PMID: 15358778
    28. data indicate that binding sites in three PDI domains, a, b', and a', contribute to efficient C-P4H tetramer assembly PMID: 15590633
    29. PDI can be S-nitrosated and PDI-SNO can be denitrosated by PDI suggesting that this enzyme could be intimately involved in the transport of intracellular NO equivalents to the cell surface. PMID: 15611098
    30. Data show that protein disulfide isomerase can switch its conformation from dimer to tetramer in its functions as a foldase. PMID: 15695804

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  • 相關疾病：
    Cole-Carpenter syndrome 1 (CLCRP1)
  • 亞細胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 數據庫鏈接：

    HGNC: 8548

    OMIM: 112240

    KEGG: hsa:5034

    STRING: 9606.ENSP00000327801

    UniGene: Hs.464336



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