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  4. Human protein Red (IK) ELISA kit

Human protein Red (IK) ELISA kit

  • 中文名稱：
    人RED蛋白(IK)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011570HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人RED蛋白(IK)酶聯(lián)免疫試劑盒（CSB-EL011570HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IK含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，廣泛應(yīng)用于基礎(chǔ)研究中的蛋白質(zhì)互作分析、疾病模型構(gòu)建、藥物作用機制探索等方向，特別適用于癌癥生物學(xué)、干細胞分化研究及分子病理學(xué)等領(lǐng)域的定量檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    chondrosarcoma associated protein 2 ELISA Kit; CSA2 ELISA Kit; Cytokine IK ELISA Kit; IK ELISA Kit; IK cytokine down regulator of HLA II ELISA Kit; IK factor ELISA Kit; IK Gene ELISA Kit; prer protein ELISA Kit; Protein Red ELISA Kit; Protein RER ELISA Kit; RD element ELISA Kit; RED protein ELISA Kit; RED_HUMAN ELISA Kit; RER ELISA Kit
  • 縮寫：
    IK
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-94
    1:2 Average % 101
    Range % 98-104
    1:4 Average % 92
    Range % 85-96
    1:8 Average % 98
    Range % 94-102
  • 回收率：
    The recovery of human IK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-94
    EDTA plasma (n=4) 93 86-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.909 2.965 2.937 2.763
    800 2.215 2.332 2.274 2.100
    400 1.462 1.487 1.475 1.301
    200 0.900 0.923 0.912 0.738
    100 0.555 0.564 0.560 0.386
    50 0.363 0.378 0.371 0.197
    25 0.243 0.249 0.246 0.072
    0 0.173 0.174 0.174 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in pre-mRNA splicing as a component of the spliceosome. Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species. Required for normal mitotic cell cycle progression. Recruits MAD1L1 and MAD2L1 to kinetochores, and is required to trigger the spindle assembly checkpoint. Required for normal accumulation of SMU1.; (Microbial infection) Required, together with SMU1, for normal splicing of influenza A virus NS1 pre-mRNA, which is required for the production of the exportin NS2 and for the production of influenza A virus particles. Not required for the production of VSV virus particles.
  • 基因功能參考文獻：
    1. IK inhibits Aurora B activation through recruiting PP2A into IK and Aurora B complex. PMID: 26906715
    2. Data suggest an indirect radiosensitivity of hIK channels to low-dose photon irradiation with an impact on cell differentiation. PMID: 25277267
    3. Data suggest that two human spliceosomal factors, RED and SMU1, are recruited to control expression and alternative splicing of viral exportins via PB2 (polymerase PB2) of influenza A virus (H3N2) and are required for efficient viral multiplication. PMID: 24945353
    4. IK is an essential protein for achieving correct mitotic progress through the regulation of mitotic kinases and phosphatases PMID: 24996188
    5. RED is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint. PMID: 22351768
    6. IK gene expression is decreased in follicular variant of papillary thyroid carcinoma. PMID: 21509594
    7. At least three genes, orthologous to loci in this LD block, HBEGF, IK, and SRA1, result independently in a phenotype of myocardial contractile dysfunction when their expression is reduced with morpholino antisense reagents. PMID: 19064678

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  • 亞細胞定位：
    Nucleus. Nucleus, nucleoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    RED family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5958

    OMIM: 600549

    KEGG: hsa:3550

    STRING: 9606.ENSP00000396301

    UniGene: Hs.421245



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