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中文名稱:人前列環素(PGI2)酶聯免疫試劑盒
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貨號:CSB-E09591h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人前列環素(PGI2)酶聯免疫試劑盒(CSB-E09591h)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的PGI2含量。PGI2即前列環素,是一種具有強大擴血管及抗血小板聚集作用的生物活性物質。可激活腺苷酸環化酶,升高血小板內cAMP水平,抑制血小板聚集并舒張血管,在心血管疾病等方面的防治研究上具有重要意義。試劑盒檢測范圍為50 pg/mL-2000 pg/mL,該產品適用于心血管機制研究、藥物對血管內皮功能影響的體外評估,以及炎癥或血栓性疾病模型中PGI2動態變化的定量分析,為探索血管調節機制、抗血小板藥物開發等科研方向提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:PGI2
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma
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檢測范圍:50 pg/mL-2000 pg/mL
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靈敏度:50 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Biochemicals
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 86-101 1:2 Average % 94 Range % 86-100 1:4 Average % 94 Range % 91-96 1:8 Average % 95 Range % 85-105 -
回收率:
The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 88-98 EDTA plasma (n=4) 96 92-100 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 0 2.666 2.517 2.592 50 1.284 1.235 1.260 150 0.625 0.641 0.633 460 0.349 0.365 0.357 1000 0.221 0.229 0.225 2000 0.128 0.132 0.130 -
數據處理:
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貨期:3-5 working days
引用文獻
- α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet K Sztolsztener,Biochimica et biophysica acta. Molecular basis of disease,2022
- Donor-dependent variation of human umbilical cord blood mesenchymal stem cells in response to hypoxic preconditioning and amelioration of limb ischemia Insung Kang.et al,Experimental & Molecular Medicinevolume,2018
- A new approach to an old hypothesis; phototherapy does not affect ductal patency via PGE2 and PGI2 Surmeli-Onay O et al,J Matern Fetal Neonatal Med,2014
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