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Human procollagen type III,HPCⅢ ELISA kit

  • 中文名稱:
    人Ⅲ型前膠原(HPCⅢ)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E11989h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅲ型前膠原(HPCⅢ)酶聯(lián)免疫試劑盒(CSB-E11989h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清樣本中的HPCⅢ含量。試劑盒檢測(cè)范圍為25 ng/mL-300 ng/mL,適用于體外研究領(lǐng)域,如通過(guò)檢測(cè)動(dòng)物模型或細(xì)胞培養(yǎng)上清中的HPCⅢ水平,探索肝纖維化發(fā)生機(jī)制、篩選抗纖維化藥物活性,或用于基礎(chǔ)醫(yī)學(xué)研究中組織修復(fù)與纖維化相關(guān)疾病的病理分析。為肝病研究、藥物開發(fā)及分子生物學(xué)實(shí)驗(yàn)提供可靠的數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名:
    procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
  • 縮寫:
    HPCⅢ
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum
  • 檢測(cè)范圍:
    25 ng/mL-300 ng/mL
  • 靈敏度:
    25 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<20%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 93  
    Range % 90-96  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率:
    The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-101  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    300 1.356 1.342 1.349    
    200 0.967 1.023 0.995    
    100 0.466 0.482 0.474    
    50 0.211 0.199 0.205    
    25 0.050 0.048 0.049    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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