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中文名稱:人胃泌素釋放肽前體(ProGRP)酶聯免疫試劑盒
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貨號:CSB-E09127h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人胃泌素釋放肽前體(ProGRP)酶聯免疫試劑盒(CSB-E09127h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ProGRP含量。ProGRP(胃泌素釋放肽前體)是一種在小細胞肺癌(SCLC)中特異性高表達的腫瘤標志物。它作為SCLC發生發展中的生物標志物,可能具有致癌基因的作用,并可能成為潛在的治療靶點。研究顯示,ProGRP在SCLC的診斷、療效評估和預后預測中具有重要作用。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL,適用于腫瘤標志物篩選、疾病機制研究及抗腫瘤藥物開發等科研領域,尤其為肺癌相關生物標志物探索、病理模型驗證及組織微環境分析提供可靠工具,其優化的檢測體系支持多樣本類型的高通量檢測需求,滿足實驗室基礎研究與轉化醫學應用。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:ProGRP
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:18.75 pg/mL-1200 pg/mL
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靈敏度:4.68 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 87 Range % 80-90 1:10 Average % 97 Range % 92-101 1:20 Average % 98 Range % 93-102 1:40 Average % 93 Range % 86-96 -
回收率:
The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-96 EDTA plasma (n=4) 97 91-99 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1200 2.432 2.327 2.380 2.253 600 1.894 1.886 1.890 1.763 300 1.271 1.237 1.254 1.127 150 0.721 0.692 0.707 0.580 75 0.467 0.452 0.460 0.333 37.5 0.338 0.345 0.342 0.215 18.75 0.238 0.225 0.232 0.105 0 0.129 0.125 0.127 -
數據處理:
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貨期:3-5 working days
引用文獻
- High Sensitive Immunoelectrochemical Measurement of Lung Cancer Tumor Marker ProGRP Based on TiO2-Au Nanocomposite Zheng Wei, et al,Molecules,2019
- The prognostic value of the serum neuron specific enolase and lactate dehydrogenase in small cell lung cancer patients receiving first-line platinum-based Liu X.et al,Medicine (Baltimore),2017
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