Human osteonectin,ON ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人骨粘連蛋白(ON)酶聯(lián)免疫試劑盒（CSB-E08760h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、唾液、組織勻漿樣本中的SPOCK2含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于科研場景下探究骨發(fā)育、心血管鈣化、腫瘤轉移等生物學過程中ON蛋白的動態(tài)變化，尤其適合多類型生物樣本的批量檢測需求，滿足實驗室對骨基質相關蛋白的高通量定量分析要求。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
縮寫：

SPOCK2
Uniprot No.：

Q92563
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	94
	Range %	87-98
1:40	Average %	96
	Range %	90-102
1:80	Average %	94
	Range %	88-98
1:160	Average %	97
	Range %	92-103

回收率：

The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	86	80-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May participate in diverse steps of neurogenesis. Binds calcium.
基因功能參考文獻：
1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization PMID: 21836138
2. Neurons are a major source of all of the testican family members in the normal brain. Abundant distribution of testican 2 may contribute to glioma invasion by inactivating other testican family members including N-Tes, which all inhibit MT-MMPs. PMID: 12810672
3. purification and characterization of a recombinant testican-2 expressed in insect cells; the C1q subcomponent of complement component C1 was identified as a potential interacting partner of testican-2 PMID: 17950619
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Highly expressed in brain. Also found in lung and testis.
數(shù)據(jù)庫鏈接：

HGNC: 13564

OMIM: 607988

KEGG: hsa:9806

STRING: 9606.ENSP00000321108

UniGene: Hs.523009