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Human neutrophil peptide 1-3,HNP1-3 ELISA Kit

  • 中文名稱:
    人中性粒細(xì)胞防御素1-3(HNP1-3)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E11742h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人中性粒細(xì)胞防御素1-3(HNP1-3)酶聯(lián)免疫試劑盒(CSB-E11742h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的HNP1-3含量。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,適用于探索感染性疾病機(jī)制、免疫調(diào)控研究、炎癥相關(guān)疾病模型建立等科研場(chǎng)景,例如膿毒癥、類風(fēng)濕性關(guān)節(jié)炎或腫瘤微環(huán)境中的免疫應(yīng)答研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    HNP1-3
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HNP1-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %92-102
    1:2Average %85
    Range %81-90
    1:4Average %95
    Range %87-99
    1:8Average %100
    Range %85-106
  • 回收率:
    The recovery of human HNP1-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9587-99
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.800 2.788 2.794 2.692
    501.765 1.743 1.754 1.652
    251.215 1.203 1.209 1.107
    12.50.698 0.678 0.688 0.586
    6.250.362 0.355 0.359 0.257
    3.120.262 0.246 0.254 0.152
    1.560.169 0.156 0.163 0.061
    00.104 0.100 0.102  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)



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