Human midregional pro-atrial natriuretic peptide (MR-proANP) ELISA kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人中部前心鈉肽(MR-proANP)酶聯免疫試劑盒（CSB-EQ028056HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MR-proANP含量。試劑盒檢測范圍為39 pmol/L-2500 pmol/L，該產品適用于心血管疾病機制研究、動物模型中心功能評估、藥物干預效果監測等科研領域，為探索心力衰竭、心肌缺血等疾病的分子機制提供定量分析工具。試劑盒操作流程標準化，配套完整實驗組分，支持實驗室開展高通量樣本檢測，滿足基礎醫學研究和生物標志物開發需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MR-proANP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 pmol/L-2500 pmol/L
靈敏度：

9.8 pmol/L
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-97
1:2	Average %	100
	Range %	94-105
1:4	Average %	90
	Range %	83-94
1:8	Average %	97
	Range %	91-101

回收率：

The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	102	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.