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  4. Human macrophage stimulating protein,MSP ELISA Kit

Human macrophage stimulating protein,MSP ELISA Kit

  • 中文名稱：
    人巨噬細胞刺激蛋白(MSP)酶聯免疫試劑盒
  • 貨號：
    CSB-E09062h
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人巨噬細胞刺激蛋白(MSP)酶聯免疫試劑盒（CSB-E09062h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MST1含量。MST1（哺乳動物不育系20樣激酶1）是一種在哺乳動物中與酵母Ste20激酶同源的蛋白激酶，作為Hippo抑制通路的核心組件，在果蠅發育調控中起關鍵作用。MST1通過調節細胞增殖、凋亡和器官大小等，在多種生理和病理過程中發揮重要作用。近期研究發現，MST1在結直腸癌中表達降低，與不良預后相關，可能成為結直腸癌治療的潛在靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，靈敏度為0.039 ng/mL。適用于探索MSP在免疫調節相關疾病模型中的作用機制，如腫瘤侵襲轉移研究、纖維化病理進程分析或炎癥反應動態監測，為科研人員提供穩定可靠的實驗工具。助力于基礎醫學研究與分子機制解析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    D3F15S2 ELISA Kit; DNF15S2 ELISA Kit; Hepatocyte growth factor like protein alpha chain ELISA Kit; Hepatocyte growth factor like protein ELISA Kit; Hepatocyte growth factor like protein beta chain ELISA Kit; Hepatocyte growth factor like protein homolog ELISA Kit; Hepatocyte growth factor-like protein beta chain ELISA Kit; HGFL ELISA Kit; HGFL_HUMAN ELISA Kit; Macrophage stimulating 1 (hepatocyte growth factor like) ELISA Kit; Macrophage stimulatory protein ELISA Kit; Macrophage-stimulating protein ELISA Kit; MSP ELISA Kit; MST1 ELISA Kit; NF15S2 ELISA Kit; OTTHUMP00000208927 ELISA Kit
  • 縮寫：
    MST1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    1.255

    1.265

    1.297

    1.287

    1.286

    1.317

    SD

    0.038

    0.043

    0.035

    0.044

    0.044

    0.053

    CV(%)

    4.822

    5.409

    4.294

    5.439

    5.446

    6.401

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MSP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:20

    Average %

    101

    Range %

    94-108

    1:40

    Average %

    105

    Range %

    99-109

    1:80

    Average %

    89

    Range %

    82-96

    1:160

    Average %

    93

    Range %

    87-99

  • 回收率：

    The recovery of human MSP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    104

    97-113

    EDTA plasma (n=4)

    100

    95-104

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.827

    2.787

    2.807

    2.704

    5

    2.314

    2.254

    2.284

    2.181

    2.5

    1.511

    1.566

    1.539

    1.436

    1.25

    0.745

    0.773

    0.759

    0.656

    0.625

    0.412

    0.402

    0.407

    0.304

    0.312

    0.250

    0.242

    0.246

    0.143

    0.156

    0.167

    0.171

    0.169

    0.066

    0

    0.101

    0.105

    0.103

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
    MST1，全名為肌肉特異性蛋白激酶1，是一種重要的信號轉導蛋白。最近的研究表明，MST1參與了多種疾病的發生和發展，包括腫瘤、心血管疾病和神經退行性疾病等。MST1還與炎癥、自噬和細胞凋亡等生物學過程相關。
  • 基因功能參考文獻：
    1. Mst1 has an important role in inhibiting the growth of NSCLC in vitro and in vivo; its antiproliferative effect is associated with induction of apoptosis. PMID: 23928732
    2. These data suggest that MSP is an effective inhibitor of inflammation and lipid accumulation in the stressed liver, thereby indicating that MSP has a key regulatory role in non-alcoholic steatohepatitis. PMID: 27609031
    3. Identify MST1/MSP as a mitogen for tracheal basal cells. PMID: 25551685
    4. MSP appears to promote the migration of fibroblasts, enhances collagen synthesis and remodeling, and effectively improves wound healing. PMID: 25315688
    5. Elevated serum levels of MST1 were found in subjects with excessive alcohol use. PMID: 25704570
    6. These results support the hypothesis that the alpha-chain of MSPalphabeta mediates RON dimerization. PMID: 25193665
    7. functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele PMID: 24409221
    8. Results suggest that the [AA] genotype of the common MST1 variant rs3197999 enhances genetic risk of sporadic extrahepatic cholangiocarcinoma irrespective of primary sclerosing cholangitis status. PMID: 23422030
    9. MSP may be one of the major determinants that affects the properties of tumor stroma and that produces a permissive microenvironment to promote cancer metastasis PMID: 23011677
    10. The present results refine the known genetic architecture in primary sclerosing cholangitis by confirming MST1 locus association. PMID: 22554193
    11. the missense SNP impairs MSP function by reducing its affinity to RON and perhaps through a secondary effect on in vivo concentration arising from reduced thermodynamic stability, resulting in down-regulation of the MSP/RON signaling pathway. PMID: 22087277
    12. HAT cleaves proMSP at the physiological activation site PMID: 22245154
    13. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
    14. These findings suggest that the MSP/RON signaling pathway may be regulated by hepsin in tissue homeostasis and in disease pathologies, such as in cancer and immune disorders. PMID: 21875933
    15. considerable number of Merkel cell carcinoma cases expressed both RON and MSP, while Merkel cells do not express these molecules PMID: 21723047
    16. MSP-induced RSK2 activation is a critical determinant linking RON signaling to cellular EMT program. PMID: 21619683
    17. the oncogenic effect of BRAF(V600E) is associated with the inhibition of MST1 tumor suppressor pathways, and that the activity of RASSF1A-MST1-FoxO3 pathways determines the phenotypes of BRAF(V600E) tumors. PMID: 21249150
    18. results suggest that MSP induces uPAR expression via MAPK, AP-1 and NF-kappaB signaling pathways PMID: 21081472
    19. Observational study and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20014019
    20. These results suggest that activation of RON by macrophage-stimulating protein inhibits LPS-induced macrophage Cox-2 expression. PMID: 12177064
    21. Data show that macrophage stimulating protein (MSP) and its receptor (Ron) induce phosphorylation of both Ron and alpha6beta4 integrin, and result in activation of alpha3beta1 integrin. PMID: 12919677
    22. macrophage-stimulating protein-responsive cell growth in culture is suppressed by the ron-sema domain PMID: 14597639
    23. Macrophage stimulating protein and its receptor RON are involved in the pathophysiology of endometriosis. PMID: 15764806
    24. repression of MSP gene expression by mutant p53 may contribute to oncogenesis in a cell type-specific manner PMID: 16170349
    25. overexpression of MSP, MT-SP1, and MST1R was a strong independent indicator of both metastasis and death in human breast cancer PMID: 17456594
    26. Cell migration and production of inflammatory cytokines by the brain are enhanced by MSP stimulation in primary microglia. PMID: 18480548
    27. The five gene transcripts (aldolase B, elafin, MST-1, simNIPhom and SLC6A14) were changed in patients with ulcerative colitis, and were related to the disease activity. PMID: 18700007
    28. Gene-centric association mapping of chromosome 3p implicates MST1 in IBD pathogenesis. PMID: 19079170
    29. Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
    30. The effect of BSN-MST1 locus on Crohn's disease predisposition was replicated, but no influence on ulcerative colitis or multiple sclerosis predisposition could be detected PMID: 19657358

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  • 相關疾?。?/div>
    MST1 variant Cys-689 may be associated with inflammatory bowel disease (IBD), a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is unsure whether Cys-689 itself or a variation in linkage disequilibrium with Cys-689 is responsible for the association with IBD.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 數據庫鏈接：

    HGNC: 7380

    OMIM: 142408

    KEGG: hsa:4485

    STRING: 9606.ENSP00000414287

    UniGene: Hs.349110



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