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  4. Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

  • 中文名稱：
    人巨噬細胞炎性蛋白5(MIP-5)酶聯免疫試劑盒
  • 貨號：
    CSB-E07997h
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人巨噬細胞炎性蛋白5(MIP-5)酶聯免疫試劑盒（CSB-E07997h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL15含量。CCL15，又稱巨噬細胞抑制蛋白5（MIP-5）、白細胞趨化因子1（Lkn-1）和人CC趨化因子2（HCC-2），是一種小細胞因子，屬于CC趨化因子家族。CCL15在肝臟、小腸、結腸和肺的某些白細胞和巨噬細胞中表達，具有趨化中性粒細胞、單核細胞和淋巴細胞的特性，通過與CCR1和CCR3受體結合，在免疫細胞募集和炎癥性疾病發展中起關鍵作用。研究機制表明，CCL15在腫瘤微環境中通過調節相關基因表達，影響腫瘤生長和轉移。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為7.81 pg/mL。該產品為炎癥機制研究、疾病模型評估及藥物干預實驗提供有效工具，特別適用于探索MIP - 5在病理過程中的調控網絡、驗證體外細胞實驗數據或評估動物模型治療效果。配套提供標準品、預混試劑及操作流程說明，支持常規實驗室開展定量檢測，滿足科研領域對趨化因子信號通路研究的實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    C-C motif chemokine 15 ELISA Kit; C-C motif chemokine ligand 15 ELISA Kit; CC chemokine 3 ELISA Kit; CCL15 ELISA Kit; CCL15(29-92) ELISA Kit; CCL15_HUMAN ELISA Kit; Chemokine (C C motif) ligand 15 ELISA Kit; Chemokine CC-2 ELISA Kit; Chemokine CC2 ELISA Kit; HCC-2 ELISA Kit; HCC2 ELISA Kit; HMRP 2B ELISA Kit; HMRP2B ELISA Kit; Leukotactin 1 ELISA Kit; Leukotactin-1 ELISA Kit; LKN-1 ELISA Kit; LKN1 ELISA Kit; Macrophage inflammatory protein 5 ELISA Kit; MIP 1D ELISA Kit; MIP-1 delta ELISA Kit; MIP-1D ELISA Kit; MIP-5 ELISA Kit; MIP1 delta ELISA Kit; MIP1d ELISA Kit; MIP5 ELISA Kit; MRP 2B ELISA Kit; Mrp-2b ELISA Kit; Mrp2b ELISA Kit; NCC 3 ELISA Kit; NCC-3 ELISA Kit; NCC3 ELISA Kit; New CC chemokine 3 ELISA Kit; OTTHUMP00000163955 ELISA Kit; SCYA15 ELISA Kit; SCYL3 ELISA Kit; Small inducible cytokine A15 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 15 ELISA Kit; Small-inducible cytokine A15 ELISA Kit; SY15 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    244.575

    250.280

    257.432

    237.463

    249.328

    256.000

    SD

    0.024

    0.031

    0.037

    0.026

    0.035

    0.041

    CV(%)

    3.352

    4.258

    4.980

    3.709

    4.821

    5.541

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:4

    Average %

    86

    Range %

    81-89

    1:8

    Average %

    95

    Range %

    91-99

    1:16

    Average %

    87

    Range %

    83-92

    1:32

    Average %

    105

    Range %

    101-109

  • 回收率：

    The recovery of human MIP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    99

    95-103

    Heparin plasma (n=4)

    91

    89-93

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.652

    2.701

    2.677

    2.505

    1000

    1.945

    1.932

    1.939

    1.767

    500

    1.213

    1.204

    1.209

    1.037

    250

    0.741

    0.735

    0.738

    0.566

    125

    0.451

    0.432

    0.442

    0.270

    62.5

    0.330

    0.342

    0.336

    0.164

    31.25

    0.266

    0.257

    0.262

    0.090

    0

    0.170

    0.173

    0.172

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
  • 基因功能參考文獻：
    1. CCL15 secreted from SMAD4-deficient xenografted human colorectal cancer cells recruited CCR1(+) cells, promoting their metastatic activities to the lung. Lung metastases from colorectal cancer patients revealed that CCL15 expression correlated with loss of SMAD4. CCL15-positive metastases recruited approximately 1.9 times more numbers of CCR1(+) cells than CCL15-negative metastases. PMID: 27492974
    2. CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis. PMID: 26501423
    3. Orthotopic animal models of HCC were established to investigate the role of CCL15 in hMSCs migration toward HCC in vivo. Both histological and flow cytometric analysis showed that significantly fewer hMSCs localized within 97H-CCL15-shRNA xenografts compared with 97H-green fluorescent protein xenografts after intravenous delivery PMID: 26763650
    4. Loss of SMAD4 was significantly associated with CCL15 expression. PMID: 26341919
    5. follicular thyroid carcinoma (FTC) might induce tumor associated macrophages (TAMs) infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from follicular adenoma PMID: 26875556
    6. two inflammatory cytokines, MIP-1delta and MIP-3alpha, are able to increase mesenchymal stromal cells migration in vitro PMID: 25579056
    7. we investigated a role of CCL15 in upregulating ICAM-1 in endothelial cells PMID: 23690481
    8. CCL15 is elevated in human asthma and correlates with disease severity . PMID: 22092970
    9. Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551
    10. increased secretion by macrophages during Mycobacterium tuberculosis infection PMID: 20016943
    11. truncation of NH2-terminal amino acid residues increases agonistic potency on CC chemokine receptors 1 and 3 PMID: 11832479
    12. Lkn-1 activates the ERK pathway by transducing the signal through G(i)/G(o) protein, PLC, PKC delta and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes PMID: 12759139
    13. These observations demonstrate that the two NF-kappaB binding sites are essential for phorbol myristate acetate-induced leukotactin-1 (Lkn-1)/CCL15 expression in human monocytes. PMID: 15179048
    14. CCL15(25-92) has in vitro and in vivo angiogenic activity PMID: 15251437
    15. alanine-aspartic acid residues preceding the first cysteine at the NH(2)-terminus are essential for the binding and biological activity of leukotactin-1 PMID: 15894113
    16. Compared with full-length CCL15, proteolytically processed CCL15 isoforms with N-terminal deletions display increased potency to induce calcium fluxes and chemotactic activity on monocytes and to induce adhesiveness of mononuclear cells to fibronectin. PMID: 16034099
    17. Transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells. PMID: 16364464
    18. Results point to an involvement of the CCL15-CCR1 axis in the pathophysiology of chronic renal failure. PMID: 16737685
    19. factor NF-kappaB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1 PMID: 17296613

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
  • 數據庫鏈接：

    HGNC: 10613

    OMIM: 601393

    KEGG: hsa:6359

    STRING: 9606.ENSP00000432034

    UniGene: Hs.272493



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