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Human leucocyte antigen B27,HLA-B27 ELISA Kit

  • 中文名稱:
    人類白細(xì)胞抗原B27(HLA-B27)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08607h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人類白細(xì)胞抗原B27(HLA-B27)酶聯(lián)免疫試劑盒(CSB-E08607h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、腦脊液(CSF)樣本中的HLA-B27含量。HLA-B27是人類白細(xì)胞抗原B27。它與脊柱關(guān)節(jié)病尤其是強(qiáng)直性脊柱炎密切相關(guān)。研究機(jī)制上,HLA-B27可能通過分子模擬、關(guān)節(jié)源性肽及免疫調(diào)節(jié)異常等機(jī)制,引發(fā)機(jī)體異常免疫反應(yīng),導(dǎo)致關(guān)節(jié)等部位的炎癥與病變。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,可為免疫性疾病機(jī)制研究、炎癥反應(yīng)通路探索以及HLA - B27表達(dá)水平與疾病相關(guān)性分析提供可靠工具,尤其適用于神經(jīng)免疫、風(fēng)濕病學(xué)等領(lǐng)域的體液樣本檢測需求,其多樣本類型的兼容性為跨組織液比較研究創(chuàng)造了便利條件。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    HLA-B27
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine, cerebrospinal fluid (CSF)
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 80-98  
    1:2 Average % 86  
    Range % 80-96  
    1:4 Average % 96  
    Range % 92-100  
    1:8 Average % 100  
    Range % 96-110  
  • 回收率:
    The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.730 2.612 2.671 2.563  
    10 2.361 2.241 2.301 2.193  
    5 1.702 1.638 1.670 1.562  
    2.5 0.933 0.871 0.902 0.794  
    1.25 0.534 0.522 0.528 0.420  
    0.625 0.324 0.318 0.321 0.213  
    0.312 0.243 0.236 0.240 0.132  
    0 0.109 0.107 0.108    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 相關(guān)疾病:
    Spondyloarthropathy 1 (SPDA1)
  • 蛋白家族:
    MHC class I family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 4932

    OMIM: 106300

    UniGene: Hs.654404



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