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Human lactate dehydrogenase A (LDHA) ELISA kit

  • 中文名稱:
    人乳酸脫氫酶A (LDHA)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E17850h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人乳酸脫氫酶A (LDHA)酶聯(lián)免疫試劑盒(CSB-E17850h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的LDHA含量。LDHA即乳酸脫氫酶A,在糖酵解代謝通路中發(fā)揮關(guān)鍵作用,可催化丙酮酸和乳酸的可逆轉(zhuǎn)換。其表達(dá)異常與腫瘤細(xì)胞的能量代謝重編程密切相關(guān),是腫瘤治療的重要潛在靶點(diǎn),研究聚焦于開發(fā)其抑制劑以阻斷腫瘤代謝,抑制腫瘤生長(zhǎng)。試劑盒檢測(cè)范圍為31.25 mU/mL-2000 mU/mL,適用于體外培養(yǎng)細(xì)胞能量代謝評(píng)估、動(dòng)物模型代謝動(dòng)態(tài)分析、抗腫瘤藥物篩選等科研場(chǎng)景,特別為研究腫瘤瓦伯格效應(yīng)、缺氧應(yīng)激反應(yīng)或代謝相關(guān)信號(hào)通路提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名:
    Cell proliferation-inducing gene 19 protein ELISA Kit; GSD11 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; Lactate dehydrogenase A ELISA Kit; Lactate dehydrogenase B ELISA Kit; Lactate dehydrogenase c variant 1 ELISA Kit; Lactate dehydrogenase c variant 3 ELISA Kit; Lactate dehydrogenase c variant 4 ELISA Kit; Lactate dehydrogenase C4 ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; Lactate dehydrogenase M ELISA Kit; LDH A ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH M ELISA Kit; LDH muscle subunit ELISA Kit; LDH-A ELISA Kit; LDH-M ELISA Kit; LDH1 ELISA Kit; ldha ELISA Kit; LDHA_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHM ELISA Kit; MS1111 ELISA Kit; PIG19 ELISA Kit; Proliferation inducing gene 19 ELISA Kit; Proliferation-inducing gene 19 ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-59 ELISA Kit; TRG 5 ELISA Kit; TRG5 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍:
    31.25 mU/mL-2000 mU/mL
  • 靈敏度:
    7.81 mU/Ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human LDHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %88
    Range %81-92
    1:2Average %99
    Range %94-102
    1:4Average %101
    Range %97-105
    1:8Average %94
    Range %88-98
  • 回收率:
    The recovery of human LDHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-96
    EDTA plasma (n=4)9694-98
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    20002.101 1.952 2.027 1.914
    10001.676 1.734 1.705 1.592
    5001.252 1.238 1.245 1.132
    2500.768 0.758 0.763 0.650
    1250.566 0.568 0.567 0.454
    62.50.328 0.321 0.325 0.212
    31.250.179 0.185 0.182 0.069
    00.114 0.112 0.113  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn):
    1. The study reveals that miR-33b plays a suppressive role in the regulation of osteosarcoma cell proliferation through direct targeting LDHA. PMID: 30213286
    2. hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation. PMID: 28516914
    3. the results of the present study demonstrated that miR199a3p can inhibit LDHA expression by downregulating Sp1, and provided mechanistic evidence supporting the existence of a novel miR199a3p/Sp1/LDHA axis and its critical contribution to aerobic glycolysis in testicular cancer cells. PMID: 30015851
    4. High LDHA expression is associated with Non-Small Cell Lung Cancer. PMID: 29321091
    5. This is the first study from this highly prevalent region of Head neck cancer showing that serum LDH could be regarded as a biomarker for malignant and premalignant conditions of the head and neck. PMID: 30197328
    6. High LDH expression is associated with small cell lung cancer. PMID: 29970686
    7. The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices. PMID: 23703029
    8. miR-199a-3p is a major metabolic regulator in tumor suppression and miR-199a-3p may downregulate metabolic genes (LDHA, PGK1, MCT1, TIGAR) through the transcription factor Sp1. PMID: 27432288
    9. The expression of LDH-5 and hypoxia-inducible factor (HIF) 1alpha in Non-Hodgkin's lymphoma, was examined. PMID: 24086384
    10. Results show that LDHA is highly expressed in triple negative breast cancer (TNBC) and correlated with a poor outcome. Its 3'UTR is targeted by miR-34a. Also, LDHA along with PDL1 seem to act as ceRNAs to promote the expression and function of each other through regulation of miR-34a in TNBC. PMID: 28915924
    11. LDHA siRNA can inhibit cell proliferation, induce apoptosis, reduce invasion and migration in renal cell carcinoma cells. PMID: 27027898
    12. JMJD2A regulated aerobic glycolysis by regulating LDHA expression. Therefore, the novel JMJD2A-LDHA signaling pathway could contribute to the Warburg effects in NPC progression. PMID: 28693517
    13. The present study proved that HIF1/2alpha could activate LDHA expression in human pancreatic cancer cells, and high expression of LDHA promoted the growth and migration of pancreatic cancer cells. PMID: 28193910
    14. Pretreatment LDH levels had noticeable prognostic value in advanced pancreatic ductal adenocarcinoma (PDAC) patients who received subsequent chemotherapy. Tackling elevated LDH levels before the initiation of chemotherapy might be a promising measure for improving overall survival of patients after treatment for their advanced PDAC. PMID: 28056913
    15. Authors demonstrated that knock down of LDHA with siRNA or inhibition of LDHA activity with a LDHA specific inhibitor (FX-11), could sensitize PC-3RR cells to radiotherapy with reduced epithelial-mesenchymal transition, hypoxia, DNA repair ability and autophagy, as well as increased DNA double strand breaks and apoptosis. PMID: 27708237
    16. Taken together, these results indicate that miR-142-3p could act as a tumor suppressor in HCC by targeting LDHA, suggesting new therapeutic targets for HCC treatment. PMID: 29360449
    17. Findings suggest that miR-34b-3 and miR-449a suppress the development of nasopharyngeal carcinoma (NPC) through regulation of glycolysis via targeting lactate dehydrogenase A.(LDHA) and may be potential therapeutic targets for the treatment of NPC. PMID: 27458165
    18. Results provide evidence that miR-200b acts as a tumor suppressor in glioma through the inhibition of LDHA both in vitro and in vivo. PMID: 27374173
    19. our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID: 27150057
    20. serum lactate dehydrogenase has a role in predicting the outcome of resected gastric cancer and construct prognostic nomograms for risk prediction PMID: 27223065
    21. LDHA phosphorylation and activation provide pro-invasive, anti-anoikis and pro-metastatic advantages to cancer cells, suggesting that Y10 phosphorylation of LDHA may represent a promising therapeutic target and a prognostic marker for metastatic human cancers. PMID: 28218905
    22. miR-30a-5p may function as a tumor suppressor in breast cancer through the inhibition of LDHA expression and glycolysis. PMID: 28461244
    23. Overexpression of LDHA can be a marker of poor prognosis in cholangiocarcinoma patients PMID: 27615379
    24. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
    25. Data indicate that lactate dehydrogenase A (LDHA) is involved in the transcription of histone 2B gene. PMID: 28257841
    26. LDHA downregulation is involved in the apoptotic effect of diallyl trisulfide in triple-negative breast cancer. PMID: 27566995
    27. this study shows that low levels of LDH correlate to a better anti-CTLA-4 immunotherapy response and survival in advanced melanoma patients PMID: 27728898
    28. alternative isoforms of LDH in cancer cells produce lactic acid, when LDHA is silenced or inhibited PMID: 28314283
    29. Overexpression of PKM2 and LDH5 associates with key clinicopathological features and unfavourable prognosis in tongue squamous cell carcinoma. PMID: 24762230
    30. These results suggest LDH-A and/or lactate as common elements at the cross-road between cancer cell metabolism, tumor progression and inflammation PMID: 27622920
    31. High serum LDH is associated with OS and PFS in patients with urinary system cancer, and it is an effective biomarker of prognosis in patients with urologic cancer. PMID: 27710971
    32. High serum LDH level is associated with cancer. PMID: 27511116
    33. The number of in advanced-stage diffuse large B cell lymphoma patients with elevated serum lactate dehydrogenase who achieved complete response (CR) after R-CHOP in the non autologous hematopoietic stem cell transplantation (ASCT) group was higher than that in the ASCT group. PMID: 27324387
    34. this study shows that elevated serum LDH levels are associated with unfavorable prognosis in nasopharyngeal carcinoma patients treated with intensity-modulated radiotherapy PMID: 26928265
    35. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
    36. IDO, through GCN2 kinase activation, downregulates the levels of TCRcomplex tchain and cMyc, resulting in the suppression of Tcell proliferation and a reduction in the levels of LDHA and GLS2 PMID: 26647830
    37. Findings provide evidence of the cellular functions of lactate dehydrogenase A in the progression of glioblastoma and suggest that lactate dehydrogenase A might act as a potential therapeutic target for glioblastoma treatment. PMID: 26694942
    38. Reanalysis of genome-wide association study and in vitro validation show that lactate dehydrogenase interacts with branched-chain amino acid metabolism. PMID: 26014429
    39. LDH and complex I play distinct roles in regulating glycolysis and cell proliferation. Inhibition of these two augments synthetic lethality in melanoma. PMID: 26484566
    40. Multivariable analysis showed that combined expression of pyruvate kinase type M2 and lactate dehydrogenase A was an independent poor prognostic marker for survival. PMID: 26989901
    41. Although lactate dehydrogenase A (LDHA) expression varies biphasically during melanoma brain metastasis formation, tumor progression and survival seem to be functionally independent of LDHA. PMID: 25791837
    42. dehydrogenase A negatively regulated by miRNAs promotes aerobic glycolysis and is increased in colorectal cancer. PMID: 26062441
    43. positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. PMID: 26721441
    44. FOXM1-LDHA signaling functioned as a stimulator of glycolysis and promoted gastric cancer progression. PMID: 26261559
    45. Mammalian photoreceptors contain dimeric and tetrameric PKM2 and LDH-A. This is consistent with the ability to switch between energy production and biosynthesis like a proliferating tissue, possibly due to demands of opsin synthesis. PMID: 26780311
    46. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
    47. These results indicate that disrupting SLC2A1, LDHA, or other regulators in cancer cell energetics is a very promising approach for new targeted therapies. PMID: 25838393
    48. High expression of LDHA is associated with gastric cancer. PMID: 25913622
    49. High LDHA expression is associated with tumor progression in prostate cancer. PMID: 25983002
    50. LDHA is downregulated by JQ1, which suppresses tumor growth in ovarian cancer PMID: 25762632

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  • 相關(guān)疾病:
    Glycogen storage disease 11 (GSD11)
  • 亞細(xì)胞定位:
    Cytoplasm.
  • 蛋白家族:
    LDH/MDH superfamily, LDH family
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 6535

    OMIM: 150000

    KEGG: hsa:3939

    STRING: 9606.ENSP00000445175

    UniGene: Hs.2795



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