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Human histidine-rich glycoprotein (HRG) ELISA kit

  • 中文名稱:
    人富組氨酸糖蛋白(HRG)酶聯免疫試劑盒
  • 貨號:
    CSB-E13159h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人富組氨酸糖蛋白(HRG)酶聯免疫試劑盒(CSB-E13159h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HRG含量。試劑盒檢測范圍為6.25 μg/mL-400 μg/mL,可為研究HRG在凝血機制異常、免疫調節失衡及相關疾病模型中的分子作用提供可靠工具,適用于基礎科研、藥物篩選及病理機制探索等非診斷性研究場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    HRG ELISA Kit; Histidine-rich glycoprotein ELISA Kit; Histidine-proline-rich glycoprotein ELISA Kit; HPRG ELISA Kit
  • 縮寫:
    HRG
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    6.25 μg/mL-400 μg/mL
  • 靈敏度:
    1.56 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HRG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %84-101
    1:2Average %95
    Range %88-102
    1:4Average %89
    Range %81-97
    1:8Average %94
    Range %85-99
  • 回收率:
    The recovery of human HRG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9487-103
    EDTA plasma (n=4)9689-106
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2AverageCorrected
    4002.604 2.497 2.551 2.409
    2001.853 1.824 1.839 1.697
    1000.987 1.030 1.009 0.867
    500.562 0.558 0.560 0.418
    250.343 0.357 0.350 0.208
    12.50.225 0.236 0.231 0.089
    6.250.175 0.181 0.178 0.036
    00.141 0.143 0.142
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.
  • 基因功能參考文獻:
    1. The HRG C633T genotype seems to be associated with gestational hypertensive disorders PMID: 29540166
    2. A novel heterozygous single base pair substitution in exon 2 of HRG gene identified in a familial early-onset deep venous thrombosis case. PMID: 29108964
    3. presence of HRG mRNA did not depend on the cancer type, on the preoperative treatment or its absence, as well as on the tumor progression stage and the presence of metastases PMID: 29536307
    4. C633T SNP had no significant effect on sperm DNA integrity PMID: 28356499
    5. once infertility is established the HRG C633T SNP seems to be important for male infertility and pregnancy rate following IVF PMID: 27210772
    6. These data highlight the complex divalent metal-dependent regulatory mechanisms that govern HRG function. PMID: 27930811
    7. HRG attenuates DNA- and RNA-mediated FXII activation, and FXI activation by FXIIa or by thrombin, suggesting that HRG down regulates the capacity of DNA and RNA to activate the intrinsic coagulation pathway. PMID: 26354857
    8. HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
    9. HRG could inhibit hepatocellular carcinoma cell proliferation via the FGF-Erk1/2 signaling pathway by reducing Erk1/2 phosphorylation. PMID: 26336134
    10. Plasma free fatty acid levels influence Zn(2+) -dependent histidine-rich glycoprotein-heparin interactions via an allosteric switch on serum albumin. PMID: 25353308
    11. mononuclear phagocytes have specific binding sites for HRG and that these cells are essential for uptake of HRG from blood and distribution of HRG in tissues. Thus, inflammatory cells mediate the effect of HRG on tumor growth and metastatic spread. PMID: 25243896
    12. Results show that HRG is a novel transcriptional target gene of FXR in human hepatoma cells, human upcyteVR primary hepatocytes and 3D human liver microtissues in vitro and in mouse liver in vivo. PMID: 25363753
    13. genetic association study in Swedish population: Data suggest SNP in HRG (rs2228243, A1042G) is associated with recurrent miscarriage in population studied; women heterozygous for HRG A1042G SNP are protected from recurrent miscarriage. [PILOT STUDY] PMID: 25064236
    14. The protein encoded by this gene has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. PMID: 18797515
    15. histidine-rich glycoprotein tissue RNA and serum protein might have a role in breast cancer PMID: 24567057
    16. HRG does not exhibit the broad interactive properties that have been reported previously, suggesting that copurification of HRG-binding partners or other impurities are responsible for some of the reported functional properties. PMID: 23576524
    17. Association between the histidine-rich glycoprotein (HRG) C633T single nucleotide polymorphism (SNP) and recurrent miscarriage was investigated. An association between homozygous carriers and recurrent miscarriage was detected. PMID: 23672470
    18. The combination of lowered HRG and uterine artery Doppler may predict preterm preeclampsia in early pregnancy. PMID: 22895448
    19. the genetic polymorphism (rs9898 C/T) in the HRG gene of a woman affects her chances of becoming pregnant after IVF PMID: 21665544
    20. the HRG-fibrin interaction may provide a novel link between coagulation, innate immunity, and inflammation PMID: 21757718
    21. by binding to factor XIIa, HRG modulates the intrinsic pathway of coagulation, particularly in the vicinity of a thrombus where platelet release of HRG and Zn(2)(+) will promote this interaction PMID: 21304106
    22. Host-produced HRG inhibits tumor growth and metastasis by skewing tumor-associated macrophages (TAM) polarization away from the M2- to a tumor-inhibiting M1-like phenotype. Skewing of TAM polarization by HRG relies substantially on downregulation of PlGF. PMID: 21215706
    23. HRG can aid the phagocytosis of necrotic cells via a heparan sulfate-dependent pathway, and this process can be regulated by the presence of certain HRG ligands, such as heparin. PMID: 20573803
    24. Single Nucleotide Polymorphisms in HRG is associated with activated partial thromboplastin time. PMID: 20303064
    25. HRG has the unique property of complexing with IgG and facilitating a proinflammatory innate immune response to promote the clearance of necrotic cells PMID: 20071662
    26. By acting as a bridge between DNA on apoptotic cells and Fc gamma RI on monocyte-derived macrophages, HRG is a key physiological mediator of apoptotic cell clearance by macrophages. PMID: 12391183
    27. Data indicate that histidine-rich glycoprotein, in combination with the zinc ion, modulates the smooth muscle cell growth response in pathophysiological states. PMID: 12764609
    28. Histidine-rich glycoprotein binds to cell-surface heparan sulfate via its N-terminal domain following Zn2+ chelation PMID: 15138272
    29. HRG acts as a soluble adaptor molecule that binds to cells at sites of tissue injury, tumor growth, and angiogenesis PMID: 15220341
    30. HPRG binds to endothelial cell surface tropomyosin which at least partially mediates the antiangiogenic effects of HPRG PMID: 15269838
    31. HRG has the unique property of selectively recognizing necrotic cells and may play an important physiological role in vivo by facilitating the uptake and clearance of necrotic, but not apoptotic, cells by phagocytes PMID: 16107330
    32. the anti-angiogenic His/Pro-rich fragment of histidine-rich glycoprotein binds to endothelial cell heparan sulfate in a Zn2+-dependent manner PMID: 16436387
    33. the histidine-rich and heparin-binding domain of HRGP mediates the antibacterial activity of the protein PMID: 17229145
    34. identified the alpha-subunit of ATP synthase as one of the HRG-binding proteins on the surface of T-cells by HRG-derived glycopeptide affinity chromatography and by a peptide mass finger printing method PMID: 19285951
    35. HRG may assist in the maintenance of normal immune function by mediating the clearance of necrotic material, inhibiting the formation of insoluble immune complexes and enhancing their ability to activate complement, resulting in faster clearance. PMID: 19674792
    36. Proteolytic cleavage of HRG by plasmin may provide a feedback mechanism to regulate the effects of HRG on the plasminogen/plasmin system and other functions of HRG. PMID: 19712047

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  • 相關疾病:
    Thrombophilia due to histidine-rich glycoprotein deficiency (THPH11)
  • 亞細胞定位:
    Secreted.
  • 組織特異性:
    Expressed in macrophages and in malignant cells. Expressed by the liver and secreted in plasma (at protein level).
  • 數據庫鏈接:

    HGNC: 5181

    OMIM: 142640

    KEGG: hsa:3273

    STRING: 9606.ENSP00000232003

    UniGene: Hs.1498



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