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Human hepatocyte growth factor,HGF ELISA kit

  • 中文名稱:
    人肝細胞生長因子(HGF)酶聯免疫試劑盒
  • 貨號:
    CSB-E04573h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人肝細胞生長因子(HGF)酶聯免疫試劑盒(CSB-E04573h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HGF含量。肝細胞生長因子(HGF)是一種多功能細胞因子,主要來源于間充質細胞,作用于上皮細胞,參與細胞生長、遷移和形態發生。HGF通過與其受體MET結合,激活下游信號通路,如PI3K-Akt和Ras-MAPK,調節多種生物學過程,包括炎癥、組織修復、血管生成和腫瘤生長。HGF/MET信號通路異常與多種疾病,尤其是腫瘤的發生和發展密切相關。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,靈敏度為3.9 pg/mL。適用于基礎醫學研究中的肝損傷修復模型評估、腫瘤侵襲轉移機制探索,或藥物研發中靶向HGF通路的干預效果驗證,為細胞信號轉導、再生醫學及癌癥生物學等領域提供可靠的定量分析支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    DFNB39 ELISA Kit; F TCF ELISA Kit; Fibroblast derived tumor cytotoxic factor ELISA Kit; Hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit; Hepatocyte growth factor ELISA Kit; Hepatocyte growth factor beta chain ELISA Kit; Hepatocyte growth factor precursor ELISA Kit; Hepatopoietin A ELISA Kit; Hepatopoietin-A ELISA Kit; Hgf ELISA Kit; HGF_HUMAN ELISA Kit; HGFB ELISA Kit; HPTA ELISA Kit; Lung fibroblast derived mitogen ELISA Kit; OTTHUMP00000161349 ELISA Kit; OTTHUMP00000206710 ELISA Kit; OTTHUMP00000206711 ELISA Kit; OTTHUMP00000206712 ELISA Kit; OTTHUMP00000206713 ELISA Kit; OTTHUMP00000206730 ELISA Kit; Scatter factor ELISA Kit; SF ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    80-98

    1:2

    Average %

    90

    Range %

    85-100

    1:4

    Average %

    95

    Range %

    92-100

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of human HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    91-108

    EDTA plasma (n=4)

    99

    94-105

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    1.873

    1.953

    1.913

    1.723

    500

    1.332

    1.307

    1.320

    1.129

    250

    0.839

    0.903

    0.871

    0.680

    125

    0.629

    0.619

    0.624

    0.434

    62.5

    0.429

    0.421

    0.425

    0.235

    31.2

    0.323

    0.335

    0.329

    0.139

    15.6

    0.259

    0.248

    0.254

    0.063

    0

    0.189

    0.193

    0.191

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    HGF (Hepatocyte Growth Factor)是一種多功能細胞因子,可以促進細胞增殖、遷移、分化和存活。近年來,HGF的研究涉及許多領域,如腫瘤生物學、肝臟再生、神經保護和免疫學等。一些研究表明,HGF在腫瘤微環境中的表達水平升高,與腫瘤細胞的轉移和治療耐藥性有關。另外,HGF對于急性和慢性腎臟損傷、胰島素抵抗和炎癥反應等疾病的發生和發展也具有一定的調節作用。
  • 功能:
    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 基因功能參考文獻:
    1. miR-449a suppresses hepatocellular carcinoma tumorigenesis by down-regulating activity in the c-Met/ERK pathway. PMID: 30108016
    2. widely stained in sclerosing stromal tumours of the ovary PMID: 29433373
    3. This novel study, in HIV-positive, preeclampsia, and normotensive pregnancies, demonstrates that HGF was two-fold higher in conducting compared to exchange villi irrespective of the pregnancy type. HIV infection does not influence HGF expression within the conducting and exchange villi. PMID: 29886319
    4. CAFderived HGF promotes angiogenesis. PMID: 29917165
    5. These results suggest that gastric cancer progression is not associated with a unique signaling pathway and that a feedback loop may exist between the HGF/c-Met and Notch1 signaling pathways, which may result in therapeutic resistance. PMID: 29781036
    6. a higher HGF serum level during hemodialysis treatment is associated with a slower loss of residual renal function PMID: 29227979
    7. prolonged treatment of single HGF/c-Met or Hh inhibitor leads to resistance to these single inhibitors, likely because the single c-Met treatment leads to enhanced expression of Shh, and vice versa. Targeting both the HGF/c-Met and Hh pathways simultaneously overcame the resistance to the single-inhibitor treatment and led to a more potent antitumor effect in combination with the chemotherapy treatment. PMID: 28864680
    8. TGF-beta negatively controls the HGF/c-MET pathway by regulating of stemness in glioblastoma. PMID: 29238047
    9. Reduction of fibroblast size upregulates HGF expression, which in turn contributes to loss of collagen, a prominent feature of aged skin. PMID: 28826691
    10. Results of our present study suggest that both IL-6 and HGF derived from Cancer-associated fibroblasts (CAFs)could contribute to the intratumoral androgen metabolism in ER-negative breast carcinoma patients. PMID: 28831645
    11. Hepatocyte growth factor as cardiovascular hormone: role of HGF in the pathogenesis of cardiovascular disease. Review. PMID: 12201209
    12. this study shows that decidual NK cells facilitate the interaction between trophoblastic and endothelial cells via VEGF-C and HGF PMID: 28653669
    13. We identify HGF, acting through the c-Met receptor, as the key polarity-inducing morphogen, which acts to activate b1-integrin-dependent adhesion. HGF and ECM-derived integrin signals co-operate via a c-Src-dependent inhibition of the RhoA-ROCK1 signalling pathway via p190A RhoGAP. PMID: 28888686
    14. High glucose activated Met receptor in HK2 cells independently of HGF, via induction of integrin a5b1 and downstream signaling. This mode of Met activation was associated with tubular cell damage and apoptosis and it may represent a novel pathogenic mechanism and a treatment target in diabetic nephropathy. PMID: 28819999
    15. results show that HGF was involved in regulating Chk1 phosphorylation, and further demonstrate that AKT activity was responsible for this HGF-induced Chk1 phosphorylation. PMID: 28573382
    16. Lymph node metastasis is strongly associated with expression status of HGF and CD133 at the deep invasive front, suggesting the usefulness of these proteins as independent predictive markers of lymph node metastasis in early gastric cancer. PMID: 28595915
    17. EGF-mediated lysosome trafficking, protease secretion, and invasion is regulated by the activity of p38 mitogen activated protein kinase (MAPK) and sodium hydrogen exchangers (NHEs). Interestingly, EGF stimulates anterograde lysosome trafficking through a different mechanism than previously reported for HGF, suggesting that there are redundant signaling pathways that control lysosome positioning PMID: 28978320
    18. HGF/c-MET pathway mediates VEGFR inhibitor resistance and vascular remodeling in NSCLC. PMID: 28559461
    19. We also propose that full-length HGF and HGF-NK1 convey desirable wound healing properties, whilst fibroblasts preferentially expressing more HGF-NK2 readily undergo TGF-beta-driven differentiation into myofibroblasts. PMID: 28837064
    20. In conclusion, these findings indicate that CMs derived from primary culture of NPC fractions of BA liver contain a large amount of active HGF, which may activate hepatic stem/progenitor cells and promote the appearance of hepatocyte-like cells or their clusters through HGF/c-Met signaling. PMID: 28364348
    21. Oxidative stress contributes to HGF-dependent pro-senescence activity of ovarian cancer cells. PMID: 28652056
    22. These results hold promise that dual targeting of HGF and MET by combining extracellular ligand inhibitors with intracellular MET TKIs could be an effective intervention strategy for cancer patients who have acquired resistance that is dependent on total MET protein. PMID: 28341789
    23. cabozantinib suppressed MMP1 expression by blocking HGF-MET signaling and that HGF-MET-MMP1 signaling is involved in the invasiveness and proliferation of BCa cells. These results suggest that cabozantinib might prove useful for future treatment of muscle-invasive BCa. PMID: 28013036
    24. The results suggested that HGF may inhibit TEMT by inhibiting AngII through the JAK2/STAT3 signaling pathway in HK2 cells and HGF may prevent apoptosis induced by AngII. The present study provides a basis for understanding the mechanisms involved in the inhibition of TEMT by HGF, which requires further investigation PMID: 28447719
    25. The identification of novel small-molecule inhibitors of microtubule polymerization highlights the role of the microtubule cytoskeleton in HGF-induced epithelial scattering. PMID: 27245142
    26. miRNA-200a expression was inverse correlation with HGF expression in stromal fibroblasts. High miRNA-200a and low HGF expression in stromal fibroblasts may predict a good prognosis in patients with non-small cell lung cancer. PMID: 27374174
    27. Activation of proHGF by St14 induces mouse embryonic stem cell differentiation. PMID: 27316827
    28. Study shows that Met and HGF have a multi-factorial relationship to the biology and outcome of breast cancer, influenced by gene copy number and protein expression, activation status, stromal environment, and cellular localisation. PMID: 27175600
    29. These results reveal the effect of HGF on the distinct pathways of eosinophil secretory functions and also provide novel insights into the role of HGF in the pathogenesis of allergic inflammation. PMID: 27552115
    30. The hepatocyte growth factor (HGF)-MET receptor tyrosine kinase signaling pathway PMID: 26822708
    31. MiRNA199a-3p suppresses tumor growth, migration, invasion and angiogenesis in hepatocellular carcinoma by targeting VEGFA, VEGFR1, VEGFR2, HGF and MMP2 PMID: 28358369
    32. High HGF expression is associated with castration-resistant progression in androgen dependent metastatic prostate cancer. PMID: 27599544
    33. miR-26a/mir-26b could suppress tumorigenesis and angiogenesis by targeting the HGF-VEGF axis, and could serve as a potential treatment modality for targeted therapy in the clinical treatment of gastric cancer. PMID: 28738343
    34. Which control critical events for colonization such as HGF/Met axis and Wwox, as therapy of bone metastasis. PMID: 28151481
    35. Data suggest that HGF, IL-20, and IL-22 in the serum and bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients before chemotherapy may be a prognostic of cancer progression. PMID: 27573644
    36. Studies indicate that the over-expression of hepatocyte growth factor (HGF) is valuable in colorectal cancer (CRC) prognosis. PMID: 28423584
    37. These results establish HGF/C-Met as a central organizing signal in blood vessel-directed tumor cell migration in vivo and highlight a promising role for C-Met inhibitors in blocking tumor cell streaming and metastasis in vivo, and for use in human trials. PMID: 27893712
    38. Furthermore, activation of HGF/Met signaling increased the expression and transcriptional activity of FOXM1, and the cross talk between FOXM1 and HGF/Met signaling promoted pancreatic ductal adenocarcinoma (PDA)growth and resistance to Met inhibition PMID: 26876216
    39. These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type PMID: 28739717
    40. Fibronectin and Hepatocyte Growth Factor were shown to be produced by lung fibroblasts and, furthermore, to enhance malignant pleural mesothelioma cell migration and invasion PMID: 28476806
    41. Glioblastoma patients with high expression of hepatocyte growth factor or unmethylated O(6)-methylguanine-DNA methyltransferase may benefit from onartuzumab plus bevacizumab chemotherapy. PMID: 27918718
    42. Plasma levels of HGF in PAH patients with mild disease were significantly higher than those in healthy controls, suggesting that plasma HGF has potential utility as a diagnostic biomarker for early PAH. PMID: 27342109
    43. Blood HGF was significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis. PMID: 27930387
    44. In summary, a decrease in VEGF-A and an increase in sVEGFR1 during chemotherapy and bevacizumab exposure can contribute to both chemotherapy (due to c-MET/b-catenin activation) and bevacizumab (due to low VEGF requirements) resistance. Because HGF levels decrease also during acquired resistance, alternative strategies to HGF-ligand inhibition should be investigated PMID: 28621236
    45. We found the roles of hepatocyte growth factor (HGF) signaling in stria vascularis development for the first time and that lack of HGF signaling in the inner ear leads to profound hearing loss in the mouse. Our findings reveal a novel mechanism that may underlie human deafness DFNB39 and DFNB97. Our findings reveal an additional example of context-dependent c-MET signaling diversity, required here for proper cellular inva PMID: 27488639
    46. Either upregulating miR-182 in retinal pigment epithelial cells or downregulating c-Met expression reduced HGF/SF-induced rises in both retinal pigment epithelial cells proliferation and chemotaxis through declines in Akt activation. PMID: 27936146
    47. Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells. PMID: 28475003
    48. Potential immune markers, including interleukin 1 receptor antagonist, interferon gamma-inducible protein 10, hepatocyte growth factor, soluble p75 tumor necrosis factor alpha receptor, vascular cell adhesion molecule 1, and matrix metalloproteinase 2, were significantly associated with significant plasma leakage during Dengue virus infection. PMID: 28077582
    49. Results indicated that HGF may promote angiogenesis by not only increasing the expression of VEGF but also by decreasing the expression of an angiostatic chemokine, CXCL10 in keratinocytes. PMID: 27718226
    50. overexpression of HGF resulted in resistance to c-MET tyrosine kinase inhibitors through an autocrine manner in gastric cancer cells PMID: 28314274

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  • 相關疾病:
    Deafness, autosomal recessive, 39 (DFNB39)
  • 蛋白家族:
    Peptidase S1 family, Plasminogen subfamily
  • 數據庫鏈接:

    HGNC: 4893

    OMIM: 142409

    KEGG: hsa:3082

    STRING: 9606.ENSP00000222390

    UniGene: Hs.396530



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