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Human hepatitis B virus e antigen (HBeAg) ELISA Kit

  • 中文名稱:
    人乙型肝炎病毒e抗原(HBeAg)酶聯免疫試劑盒
  • 貨號:
    CSB-E13557h
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    人乙型肝炎病毒e抗原(HBeAg)酶聯免疫試劑盒(CSB-E13557h)為雙抗夾心法ELISA試劑盒,定性檢測血清、血漿樣本中的HBeAg含量。HBeAg(乙型肝炎病毒e抗原)是乙型肝炎病毒的一種標志,其陽性表示病毒復制活躍,傳染性強。HBeAg的研究機制主要涉及其在免疫逃逸和慢性化感染中的作用,通過抑制機體免疫應答,HBeAg幫助HBV病毒逃避免疫系統的清除,導致慢性乙型肝炎的發生。適用于科研場景中HBeAg的定性分析,如乙肝病毒感染機制研究、抗病毒藥物效果評估或流行病學調查等。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    HBeAg
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    Request Information
  • 靈敏度:
    Request Information
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Microbiology
  • 測定原理:
    qualitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<20%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標準曲線:

    Test parameter

    specification

    test result

    Positive control

    >0.8

    2.098

    Negative control

    <0.2

    0.082

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本試劑盒所含材料:
    • A 96-well Assay plate --The 96-well plate has been pre-coated with HBeAb.
    • Negative Control (1 x 1ml) --Eliminate false positive
    • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
    • HRP-conjugated HBeAb(1 x 7ml) --Bind to the HBeAg, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Wash Buffer (20x concentrate) (1 x 30 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • Substrate B (1 x 7 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 7 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
    • An Instruction manual

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  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 貨期:
    3-5 working days

引用文獻

產品評價

相關問答

 常見問題解答
Q:

Please also advise the sulfuric acid concentration in the stop solution.

A:
Thanks for your inquiry.
The sulfuric acid concentration in the stop solution is 4N.Pls let me know if you have any further questions. Thank you.
Q:

Good afternoon. Do you have a test system for determine HBcrAg in patients with HBV infection.

A:
Thanks for your inquiry.
We have this recommended kit CSB-E13557h Human hepatitis B virus e antigen (HBeAg) ELISA Kit. I will send you the manual by e-mail.Pls let me know if you have any further questions. Thank you.


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