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  4. Human estrogen,E ELISA Kit

Human estrogen,E ELISA Kit

  • 中文名稱：
    人雌激素(E)酶聯免疫試劑盒
  • 貨號：
    CSB-E07286h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人雌激素(E)酶聯免疫試劑盒（CSB-E07286h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的E含量。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，該產品可廣泛應用于基礎醫學研究中激素動態監測、生殖生物學機制探索、環境內分泌干擾物評估，以及藥物開發過程中雌激素相關代謝通路的體外分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    E
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    25 pg/mL-1000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 88-101  
    1:2 Average % 94  
    Range % 86-100  
    1:4 Average % 97  
    Range % 94-101  
    1:8 Average % 91  
    Range % 85-96  
  • 回收率：
    The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 96 92-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 2.391 2.351 2.371    
    25 1.542 1.539 1.541    
    75 0.865 0.842 0.854    
    230 0.567 0.565 0.566    
    500 0.361 0.359 0.360    
    1000 0.156 0.153 0.155    
  • 數據處理：
  • 貨期：
    3-5 working days

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