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Human decay-accelerating factor(DAF/CD55)ELISA Kit

  • 中文名稱:
    人衰變加速因子(DAF/CD55)酶聯免疫試劑盒
  • 貨號:
    CSB-E05121h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人衰變加速因子(DAF/CD55)酶聯免疫試劑盒(CSB-E05121h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CD55含量。CD55是一種重要靶點。它是補體激活調節劑,在維持機體補體平衡中發揮關鍵作用。相關研究圍繞其在腫瘤、自身免疫病等疾病中的異常表達及功能展開,旨在通過干預CD55調節補體活性,為疾病治療提供新策略。試劑盒檢測范圍為0.39 ng/ml- 25ng/ml,靈敏度為0.09 ng/ml。該產品可應用于基礎科研領域,例如探究補體系統相關疾病模型中DAF/CD55的蛋白表達動態變化,評估炎癥微環境中補體調控機制,或用于藥物開發過程中靶向補體通路的生物標志物分析,為研究DAF/CD55在免疫調節、細胞信號傳導及病理過程中的功能提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CD 55 ELISA Kit; CD55 ELISA Kit; CD55 antigen ELISA Kit; CD55 Cromer blood group system ELISA Kit; CD55 molecule (Cromer blood group) ELISA Kit; CD55 molecule ELISA Kit; CD55 molecule; decay accelerating factor for complement (Cromer blood group) ELISA Kit; Cd55a ELISA Kit; Complement decay accelerating factor ELISA Kit; Complement decay-accelerating factor ELISA Kit; Complement decay-accelerating factor; GPI-anchored ELISA Kit; CR ELISA Kit; CROM ELISA Kit; Cromer Blood Group antigen ELISA Kit; Cromer blood group system ELISA Kit; DAF ELISA Kit; Daf-GPI ELISA Kit; DAF_HUMAN ELISA Kit; Daf1 ELISA Kit; Dcay accelerating factor for complement (CD55; Cromer blood group system) ELISA Kit; Decay accelarating factor 1; isoform CRA_a ELISA Kit; Decay accelerating factor (GPI-form) ELISA Kit; Decay Accelerating Factor for Complement ELISA Kit; Decay accelerating factor GPI-form ELISA Kit; Decay accelerating factor soluble-form ELISA Kit; GPI-DAF ELISA Kit; TC ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.39 ng/ml- 25ng/ml
  • 靈敏度:
    0.09 ng/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human DAF/CD55 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    87

    Range %

    83-91

    1:2

    Average %

    105

    Range %

    101-109

    1:4

    Average %

    94

    Range %

    88-98

    1:8

    Average %

    85

    Range %

    80-91

  • 回收率:

    The recovery of human DAF/CD55 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    88-97

    EDTA plasma (n=4)

    106

    102-109

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    25

    2.465

    2.398

    2.432

    2.258

    12.5

    1.943

    1.803

    1.873

    1.699

    6.25

    1.198

    1.171

    1.185

    1.011

    3.12

    0.742

    0.752

    0.747

    0.573

    1.56

    0.441

    0.439

    0.440

    0.266

    0.78

    0.316

    0.307

    0.312

    0.138

    0.39

    0.252

    0.247

    0.250

    0.076

    0

    0.175

    0.173

    0.174

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.; (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.; (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).; (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
  • 基因功能參考文獻:
    1. DAF is a potential molecule involving in endometrial cellular proliferation and motility PMID: 30001983
    2. The work identified two new host factors that may act as receptors for P. falciparum during invasion: CD44 and CD55. (Review) PMID: 29249333
    3. the first comprehensive analysis of variation in the CD55 gene in the context of Severe malaria. PMID: 28104671
    4. CD55 rs2564978 polymorphism may contribute to an increased risk of non-small cell lung carcinoma in Chinese population. PMID: 28008159
    5. HPLC/MS analyses of diabetic RBC glucose-modified DAF localized the sites of AGE modifications to K(125) adjacent to K(126), K(127) at the junction of CCPs2-3 and spatially near R(96), and R(100), all identified as being critical for DAF's function. Non-enzymatic DAF glycation de-regulated activation of systemic complement and T-cell activation. PMID: 28886871
    6. CD55 TT genotype was linked to H7N9/H1N1pdm09 influenza severity in a large Chinese cohort. PMID: 28510725
    7. our data show that hepatitis C virus (HCV) infection induces sCD55 expression in HCV-infected cell culture-conditioned medium and inhibits C3 convertase activity PMID: 27357152
    8. CD55 deficiency with hyperactivation of complement, angiopathic thrombosis, and protein-losing enteropathy (the CHAPLE syndrome) is caused by abnormal complement activation due to biallelic loss-of-function mutations in CD55. PMID: 28657829
    9. There is an altered pattern of CD55 and CD59 expression on RBCs of SCD Patients; however, it does not seem to play a causal role in the pathophysiology of anemia, and is unlikely to be influenced by the level of erythropoietin or other inflammatory mediators. PMID: 27667587
    10. This study indicated that the CD97 and CD55 proteins might be reliable biomarkers to predict the metastasis status and prognosis of intrahepatic cholangiocarcinoma patients. PMID: 28345461
    11. Expression of PBMC-DAF declined in patients both at mRNA and surface level and correlated negatively with the disease activity. Expression of IFN-gamma also declined in patients but correlated positively with DAF and negatively with disease activity PMID: 26906204
    12. Over expression of CD55 in brushing samples taken from Barrett's esophagus PMID: 26202380
    13. present study suggested that the expressions of CD97 antigen and decay accelerating factor DAF were both upregulated in human cervical squamous cell carcinoma PMID: 26107567
    14. conclude that CD55 expression is affected by glycemic status in human islets and plays a critical role in maintaining the conserved structure of rafts in pancreatic islets, which is similar to that of the related complement inhibitor CD59 PMID: 25797618
    15. CD55 is an essential host factor for P. falciparum invasion. CD55-null erythrocytes were refractory to invasion by all isolates of P. falciparum because parasites failed to attach properly to the erythrocyte surface. PMID: 25954012
    16. Data suggest that CD55 (but not CD59) on red blood cells is down-regulated in subjects with beta-thalassemia major as compared to control subjects; CD55 expression is intermediate on patients with beta-thalassemia intermedia. PMID: 25026028
    17. Expression of human decay-accelerating factor on intestinal epithelium of transgenic mice does not facilitate coxsackievirus B3 infection by the enteral route. PMID: 25653430
    18. Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy. PMID: 25725314
    19. Taken together, these results support the current model of coxsackievirus B3-DAF interaction and point to a specific role for viral VP1 T271 and DAF S104 at the virus-DAF interface. PMID: 25392210
    20. DAF rs10746463 polymorphism effects on the risk of developing gastric cancer in Chinese population. PMID: 25457880
    21. The presence of CD55- and/or CD59-deficient erythrocytic populations in patients with rheumatic diseases reflects an immune-mediated bone-marrow derived phenomenon. PMID: 24463881
    22. The expression levels of CD46, CD55, and CD59 were significantly higher in colon cancer tissues compared with the normal adjacent colon tissues. PMID: 24978917
    23. Anti-epidermal growth factor receptor (EGFR)-IgG3 antibody weakly promotes assembly of classical C3 convertase that is further suppressed in the presence of CD55, forcing human IgG3 to act mainly through the alternative pathway amplification route. PMID: 24973443
    24. the use of alpha-gal as antigen to induce tumor cell killing may be a potential therapeutic strategy in colon cancer and that CD55 plays a primary role in conferring resistance to lysis PMID: 24763553
    25. Hemolytic uremic syndrome evolved independently from CD55 and CD59 expression on peripheral blood cells in enteroaggregative E.coli O104:H4 infected patients. PMID: 24086391
    26. Authors showed that the PI3K/Akt pathway negatively regulated the expression of DAF on the epithelial cell surface and thus inhibited the adhesion of Dr(+) E. coli to epithelial cells. PMID: 24599886
    27. CD55 acts as a potent costimulator and activator of human naive CD4(+) cells, resulting in the differentiation of a discrete Tr1 population that inhibits T cell function in an IL-10-dependent manner and maintains the Tr1 phenotype upon restimulation. PMID: 24198281
    28. hCG plays a role in embryo-endometrium communication and affects the expression of C3 and DAF in endometrial compartments during the implantation window. PMID: 23427180
    29. The pattern of expression of the CD55(int7+) isoforms in normal and cancer tissues, were determined. PMID: 23692281
    30. hepatitis C virus (HCV) infection of hepatocytes or HCV core protein expression in transfected hepatocytes upregulated CD55 expression at the mRNA and protein levels PMID: 23658447
    31. Crry deletion from DAF-deficient mouse platelets cause abnormal platelet turnover, leading to compensatory increase in thrombopoiesis. PMID: 23390291
    32. vitamin D3 signaling may promote an anti-inflammatory response through an NF-kappaB-dependent increase in CD55 expression. PMID: 23152895
    33. HCV incorporates selectively CD59, but not CD46 or CD55, in its envelope to gain resistance to CML in serum of infected individuals PMID: 23049856
    34. Data indicate that nitric oxide downregulates decay accelerating factor (DAF) expression by inhibiting its promoter activity possibly through a decreased binding of Sp1 in association of HuR. PMID: 23176121
    35. CD55 polymorphisms are not genetic markers of aspirininduced bronchospasm, including FEV1, in the population studied. PMID: 22961402
    36. we present a concise historic prospective and a summary of accumulated knowledge on steroid hormones, DAF expression, and therapeutic implication of steroid hormone treatment--{review} PMID: 23402020
    37. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the placentas of women with acquired and inherited thrombophilia. PMID: 23042280
    38. Using biochemical and cell biological approaches in a uterine epithelial cell model (Ishikawa cells), DAF accumulates in caveolae upon exposure to nitrogen oxide. PMID: 22574734
    39. Data show that in preeclamptic women, diffuse placental C4d was associated with a significantly lower gestational age at delivery, and the mRNA expression of the complement regulatory proteins CD55 and CD59 was significantly upregulated. PMID: 23006730
    40. DiMNF can repress the cytokine-mediated induction of CD55 mRNA and protein. PMID: 22553215
    41. mumps virus (MuV) and vesicular stomatitis virus (VSV) assemble to include CD46 and CD55, two host cell regulators which inhibit propagation of complement pathways through distinct mechanisms. PMID: 22761385
    42. In conclusion, CD55 polymorphisms are associated with severe 2009 pandemic H1N1 influenza A virus infection. PMID: 22693232
    43. CD97 and CD55 showed high expression at the invasive front of gallbladder carcinoma. CD97 and CD55 expression was associated with high histologic grade, advanced pathologic T stage, clinical stage and positive venous/lymphatic invasion. PMID: 22547928
    44. It was shown that a single amino acid substitution in the capsid protein VP2 of Echovirus 11 could control the expression of the DAF-dependent phenotype. PMID: 22445689
    45. Data suggest that HBXIP upregulates CD46, CD55 and CD59 through p-ERK1/2/NF-kappaB signaling to protect breast cancer from complement-dependent cytotoxicity. PMID: 22293503
    46. CD55 levels decrease on red blood cell of all ages during new onset malarial anaemia. PMID: 22206234
    47. The lipopolysaccharide of Treponema denticola and Tannerella forsythia were the most potent for increasing the gene expression of CD55 and CD59, and to a lesser extent CD46, after a 48-h stimulation. PMID: 21545652
    48. Studies indicate that decreased expression of CD55+ and CD59+ on lymphocyte were found in 11 SLE patients accompanied by lymphocytopenia compared with controls. PMID: 21802665
    49. Women with a diagnosis of preterm labor have increased decay-accelerating factor (DAF) expression on peripheral white blood cells. Failure to elevate DAF expression may be associated with a risk of early premature delivery. PMID: 21380985
    50. results provide evidence that hDAF plays a central role in the early events of Escherichia coli Afa/Dr DAEC pathogenesis, including bacterial adherence and the establishment of cellular responses PMID: 21518786

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  • 相關疾病:
    Complement hyperactivation, angiopathic thrombosis, and protein-losing enteropathy (CHAPLE)
  • 亞細胞定位:
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 7]: Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族:
    Receptors of complement activation (RCA) family
  • 組織特異性:
    Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body flui
  • 數據庫鏈接:

    HGNC: 2665

    OMIM: 125240

    KEGG: hsa:1604

    STRING: 9606.ENSP00000316333

    UniGene: Hs.126517



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