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Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

  • 中文名稱(chēng):
    人細(xì)胞角蛋白21-1片段(CYFRA21-1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09606h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人細(xì)胞角蛋白21-1片段(CYFRA21-1)酶聯(lián)免疫試劑盒(CSB-E09606h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的CYFRA21-1含量。CYFRA21-1是一種細(xì)胞角蛋白片段,廣泛分布于正常組織中。當(dāng)細(xì)胞癌變時(shí),角蛋白被降解并釋放到血液中,CYFRA21-1水平升高。它作為肺癌,尤其是鱗狀細(xì)胞癌的標(biāo)志物,可用于診斷和預(yù)后評(píng)估。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL,本產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中腫瘤標(biāo)志物表達(dá)水平檢測(cè)、疾病機(jī)制探索或藥物開(kāi)發(fā)過(guò)程中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測(cè),為體外實(shí)驗(yàn)提供血清/血漿樣本中CYFRA21 - 1濃度的可靠數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    CYFRA21-1
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma
  • 檢測(cè)范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %86
    Range %82-90
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %94-110
    1:8Average %92
    Range %86-99
  • 回收率:
    The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
    EDTA plasma (n=4)9790-105
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.541 2.385 2.463 2.305
    102.229 2.082 2.156 1.998
    51.607 1.545 1.576 1.418
    2.50.866 0.842 0.854 0.696
    1.250.463 0.439 0.451 0.293
    0.6250.337 0.324 0.331 0.173
    0.3120.254 0.246 0.250 0.092
    00.160 0.156 0.158  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.


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