在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human cytokeratin 20,CK20 ELISA Kit

  • 中文名稱:
    人細胞角蛋白20(CK20)酶聯免疫試劑盒
  • 貨號:
    CSB-E11710h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人細胞角蛋白20(CK20)酶聯免疫試劑盒(CSB-E11710h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的KRT20含量。KRT20即角蛋白20,是一種上皮細胞角蛋白。它在胃腸道和泌尿道的上皮細胞中高度表達。研究發現其表達異常與多種腫瘤發生發展相關,比如腫瘤的增殖、侵襲與轉移,可作為腫瘤診斷與預后評估的生物標志物,在腫瘤機制研究和治療中有潛在價值。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,該產品可用于體外研究腫瘤微環境中CK20的表達動態、評估腫瘤細胞分化程度、追蹤消化道腫瘤動物模型生物標志物變化等科研場景,為腫瘤發生機制、藥物干預效果及生物標志物篩選等研究提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CD20 ELISA Kit; CK 20 ELISA Kit; CK-20 ELISA Kit; CK20 ELISA Kit; Cytokeratin-20 ELISA Kit; Cytokeratin20 ELISA Kit; K1C20_HUMAN ELISA Kit; K20 ELISA Kit; KA20 ELISA Kit; Keratin 20 ELISA Kit; keratin 20, type I ELISA Kit; keratin 21, rat, homolog of ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 20 ELISA Kit; Keratin-20 ELISA Kit; Keratin20 ELISA Kit; KRT 20 ELISA Kit; KRT 21 ELISA Kit; KRT20 ELISA Kit; KRT21 ELISA Kit; MGC35423 ELISA Kit; OTTHUMP00000164518 ELISA Kit; Protein IT ELISA Kit; type I cytoskeletal 20 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CK20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %103
    Range %96-108
    1:2Average %86
    Range %80-92
    1:4Average %95
    Range %90-100
    1:8Average %94
    Range %89-99
  • 回收率:
    The recovery of human CK20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)9690-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.766 2.868 2.817 2.673
    202.101 1.997 2.049 1.905
    101.172 1.244 1.208 1.064
    50.725 0.732 0.729 0.585
    2.50.363 0.387 0.375 0.231
    1.250.266 0.284 0.275 0.131
    0.6250.228 0.246 0.237 0.093
    00.144 0.143 0.144  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 基因功能參考文獻:
    1. Carcinoid-like/labyrinthine pattern of cell arrangement in vimentin/cytokeratin 20 expressing sebaceous neoplasms may represent a morphological phenotype of sebaceous mantles. PMID: 28027080
    2. In our cohort of specimens with equivocal urothelial atypia, very few patients without a prior diagnosis of bladder cancer progressed to diagnostic cancer (1 of 22), suggesting that CK20 and p53 staining results should be interpreted with caution in de novo atypia. PMID: 28967804
    3. results demonstrate the diagnostic value of urinary cytology and confirm CK 20 as an adjunct marker for the diagnosis of urothelial carcinoma. PMID: 28195264
    4. findings of our study imply that sequential staining of CK20 and E-cadherin prevents false-positive classification of BCC. Furthermore, our study demonstrated that p40 exhibits the same staining pattern in BCC, TE, and TB PMID: 26180934
    5. In stomach adenocarcinomas, CDH17 positively stained 64.0% (112 of 175) of tissues, compared to CK20 and CDX2, where staining was observed in only 24.6% (43 of 175) and 46.9% (82 of 175), respectively. PMID: 28029907
    6. results demonstrated upregulation of fibroblast growth factor receptor 1 and CK20 expressions in cancer bladder tissues PMID: 27259667
    7. K20 expression was detected in 6 of 75 patients with triple-negative carcinoma PMID: 26670478
    8. Circulating tumor cell CK20 and survivin expression may effectively predict overall survival in metastic colorectal cancer patients receiving chemotherapy. PMID: 26227487
    9. During postoperative follow-up, serum negative CK20 patients had significantly higher 3-year survival rates than serum positive CK20 patients. PMID: 26125781
    10. Patients with any EGFR, CK19, CK20 or survinin positivity in their peripheral blood obtain less benefit from radiotherapy. PMID: 25854400
    11. Case Report: Merkel cell carcinma that initially demonstrated cytokeratin 20 positivity but lost expression in subsequent brain metastases. PMID: 24901475
    12. Our data show that inclusion of CK20 as a biomarker efficiently improves the detection of CTCs in colorectal cancer patients PMID: 25528628
    13. The present study confirmed that CK14, but not CK20 or CK7, is expressed in urothelial carcinoma with squamous differentiation and squamous cell carcinoma of the urinary bladder. PMID: 25643514
    14. Immunostaining of CDX2 and CK20 provides beneficial information if considering whether to perform an endoscopic papillectomy (EP). PMID: 25081540
    15. High cytokeratin 20 expression is associated with invasive histological phenotype in poorly differentiated colorectal adenocarcinoma. PMID: 24403457
    16. Its association with clinical stage indicates that CK20 might have prognostic value as a marker for detection of circulating CRC cells. PMID: 23558939
    17. The CDX2/CK20 phenotype defines a distinct subgroup of colorectal cancers with microsatellite instability and poor differentiation PMID: 24025523
    18. Data show that lower survival (OS) and disease-free survival (DFS) rates were significantly associated with guanylate cyclase C (GCC) and CK20 mRNA levels. PMID: 23150200
    19. Overexpression of CK20 is associated with early-onset colorectal cancer. PMID: 23322277
    20. High cytokeratin 20 mrna expression is associated with lymphatic metastasis in colon cancer. PMID: 22752373
    21. Pouch/peripouch and UC-associated adenocarcinoma had a comparable positive rate for CK7, CK20, and CDX2 by immunohistochemistry. PMID: 22895272
    22. HER2 and CK20 were seen exclusively in the mucinogenic proliferations in congenital pulmonary airway malformations. PMID: 22348416
    23. CEA mRNA, CK20 mRNA, and serum CEA than patients at stages I-III. PMID: 22414974
    24. Results suggest that CK20 mRNA with other biomarkers in the peripheral blood of breast cancer patients may be useful to monitor the presence of disseminated tumor cells in the blood circulation and to predict the prognosis of breast cancer. PMID: 22677992
    25. Both the CK7-/CK20+ phenotype and expression of the antibody CDX2 are highly specific and sensitive markers of colorectal origin. PMID: 22268990
    26. This is the first reported study of the relationship between CK20/CK7 immunophenotype, BRAF mutations and microsatellite status in colorectal carcinomas PMID: 22361037
    27. positivity rates of CK19 and CK20 in transverse mesocolon were 48.6 and 61.2%, respectively and increased with the depth of tumor invasion PMID: 21938557
    28. Keratin 20 expression is common in colorectal carcinoma. PMID: 22237712
    29. The expressions of CK7 and CK20 in nasal polyps were analyzed. PMID: 22119824
    30. Cytokeratin 20-positive hepatocellular carcinoma PMID: 22073364
    31. CK20 and VEGF expressions in peripheral blood of colorectal carcinoma patients are promising molecular markers for disease progression and metastasis. PMID: 20395351
    32. Merkel cell carcinoma admixed with squamous cell carcinoma can be cytokeratin 20-negative and thyroid transcription factor-1-positive [case report] PMID: 21775107
    33. Our results along with the data from the literature indicate that CK7/CK20 expression may be of clinical significance. PMID: 21574103
    34. SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas. PMID: 21677534
    35. A considerable number of colorectal carcinomas showed no immunoreactivity to CK20. PMID: 21282015
    36. The immunohistochemical subtypes based on CK20and MUC1 expression correlated with ampullary tumor progression. PMID: 21106111
    37. Case Report: CK7+/CK20- Merkel cell carcinoma presenting as inguinal subcutaneous nodules with subsequent epidermotropic metastasis. PMID: 20574624
    38. MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer. PMID: 19914229
    39. CK20 expression is associated with the progression of breast cancer. PMID: 19664394
    40. The expression level of CK20 mRNA in the peripheral blood in patients with gastric cancer declines after postoperative adjuvant chemotherapy. PMID: 19145500
    41. CK20 expression modified in H. pylori chronic gastritis PMID: 11642721
    42. expression in lymph nodes correlates with poor prognosis in colorectal cancer PMID: 11844829
    43. expression pattern is unique to Barrett's esophagus PMID: 11857318
    44. Changing pattern of cytokeratin 7 and 20 expression from normal epithelium to intestinal metaplasia of the gastric mucosa and gastroesophageal junction PMID: 11962749
    45. The detection of cancer metastasis in the lymph nodes in colon carcinoma is almost doubled (21.9% vs 11.1%) by CK-20 mRNA PMID: 12515621
    46. CK 20 mRNA identification by RT-PCR is reliable and may be useful for early diagnosis in peritoneal dissemination of colon cancer PMID: 12636102
    47. It is possible to apply a simple and reliable method for the detection of circulating tumor cells based on cytokeratin-20 and prostate stem cell antigen RT-PCR assays in gastrointestinal cancers. PMID: 12894563
    48. Down regulation of cytokeratin 20 is associated with transitional and squamous cell carcinoma of the bladder PMID: 12954496
    49. The combined expression of CK7 and CK20 has a low specificity in the distinction between esophageal and cardiac (stomach) adenocarcinomas. PMID: 14631371
    50. Alteration of CK7 and CK20 expression profile that occurs early in small intestinal tumorigenesis. PMID: 15371952

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Intermediate filament family
  • 組織特異性:
    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of t
  • 數據庫鏈接:

    HGNC: 20412

    OMIM: 608218

    KEGG: hsa:54474

    STRING: 9606.ENSP00000167588

    UniGene: Hs.84905



主站蜘蛛池模板: 无码人妻丰满熟妇区免费| 国内野外强奷在线视频| 中文天堂最新版资源www官网| 亚洲丰满熟妇在线播放电影全集| 伊人久久大香线蕉综合75| 国产成人a视频高清在线观看| 久久久久无码国产精品不卡| 亚洲色成人www永久网站| 国产精品一区二区av| 亚洲色欲天天天堂色欲网| 国产高清精品软件丝瓜软件| 无码午夜福利免费区久久| 国产卡一卡二卡三无线乱码新区| 中文字幕在线观看亚洲日韩| 又色又爽又高潮免费观看| 国产精品久久久久久无码五月| 日韩午夜理论片 中文字幕 | 久热这里只有精品99在线观看| 中文字幕日本乱码仑区在线| 日本中文字幕乱码免费| 国产专区国产av| 俄罗斯大荫蒂女人毛茸茸| 精品亚洲成a人片在线观看| 国产老熟妇精品观看| 人妻丰满av无码久久不卡| 日本熟妇色xxxxxhd| 无码ol丝袜高跟秘书在线观看| 最近更新中文字幕免费大全| 熟妇人妻系列av无码一区二区| 狠狠色噜噜狠狠狠狠777米奇| 视频在线+欧美十亚洲曰本| 人人澡人人澡人人看添av| 亚洲色www成人永久网址| 女性女同性aⅴ免费观女性恋| 亚洲人成网站日本片| 少妇爆乳无码专区网站| 四虎影视在线观看2413| 97午夜理论片在线影院| 好男人www社区视频在线资源| 亚洲欧美日韩另类精品一区| 激情内射日本一区二区三区|