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  4. Human cystathionine β-synthase(CBS) ELISA Kit

Human cystathionine β-synthase(CBS) ELISA Kit

  • 中文名稱：
    人胱硫醚β-合酶(CBS)酶聯免疫試劑盒
  • 貨號：
    CSB-E13314h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人胱硫醚β-合酶(CBS)酶聯免疫試劑盒（CSB-E13314h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CBS含量。人胱硫醚β-合酶（CBS）是轉硫途徑中的關鍵酶，參與同型半胱氨酸（Hcy）的代謝，其活性與心血管疾病風險相關。研究顯示，CBS在Hcy轉化為胱硫醚的轉硫酸路徑中起重要作用，其表達上調與多種疾病如唐氏綜合癥和癌癥有關。抑制CBS可能對治療這些疾病有益。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于研究CBS在細胞代謝調控、氧化應激響應及疾病模型中的表達變化，也可用于評估藥物或基因干預對CBS活性的影響，為探究相關疾病的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    AI047524 ELISA Kit; AI303044 ELISA Kit; Beta thionase ELISA Kit; Beta-thionase ELISA Kit; Cbs ELISA Kit; Cbs cystathionine beta-synthase ELISA Kit; CBS_HUMAN ELISA Kit; Cystathionine beta synthase ELISA Kit; Cystathionine beta-synthase ELISA Kit; EC 4.2.1.22 ELISA Kit; HIP 4 ELISA Kit; HIP4 ELISA Kit; Methylcysteine synthase ELISA Kit; MGC18856 ELISA Kit; MGC18895 ELISA Kit; MGC37300 ELISA Kit; OTTHUMP00000109416 ELISA Kit; OTTHUMP00000109418 ELISA Kit; Serine sulfhydrase ELISA Kit
  • 縮寫：
    CBS
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 90-98
    1:2 Average % 106
    Range % 102-110
    1:4 Average % 96
    Range % 90-101
    1:8 Average % 88
    Range % 86-96
  • 回收率：
    The recovery of human CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 98-106
    EDTA plasma (n=4) 94 91-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 2.144 2.033 2.089 1.978
    4000 1.388 1.411 1.400 1.289
    2000 0.932 0.989 0.961 0.850
    1000 0.655 0.636 0.646 0.535
    500 0.445 0.424 0.435 0.324
    250 0.266 0.268 0.267 0.156
    125 0.212 0.221 0.217 0.106
    0 0.110 0.112 0.111 　
    .
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
  • 相關疾病：
    Cystathionine beta-synthase deficiency (CBSD)
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cysteine synthase/cystathionine beta-synthase family
  • 組織特異性：
    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • 數據庫鏈接：

    HGNC: 1550

    OMIM: 236200

    KEGG: hsa:102724560

    UniGene: Hs.533013



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