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Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

  • 中文名稱:
    人Ⅰ型膠原N末端肽(NTX)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E09233h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅰ型膠原N末端肽(NTX)酶聯(lián)免疫試劑盒(CSB-E09233h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、尿液樣本中的NTX含量。試劑盒檢測范圍為7.8 nM BCE-500 nM BCE,在骨質(zhì)疏松癥、骨轉(zhuǎn)移癌等骨骼代謝異常相關(guān)研究中具有重要價(jià)值;適用于骨代謝機(jī)制研究、抗骨質(zhì)疏松藥物開發(fā)評估、骨骼疾病動物模型分析等科研領(lǐng)域;支持研究者靈活選擇體液樣本進(jìn)行橫向比對實(shí)驗(yàn);為骨代謝相關(guān)基礎(chǔ)研究提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
  • 縮寫:
    NTX
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, urine
  • 檢測范圍:
    7.8 nM BCE-500 nM BCE
  • 靈敏度:
    1.95 nM BCE
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 98  
    Range % 94-102  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 95  
    Range % 92-99  
  • 回收率:
    The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-106  
    EDTA plasma (n=4) 92 88-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nM BCE OD1 OD2 Average Corrected  
    500 1.983 1.976 1.980 1.850  
    250 1.446 1.550 1.498 1.368  
    125 1.011 1.093 1.052 0.922  
    62.5 0.616 0.625 0.621 0.491  
    31.25 0.374 0.361 0.368 0.238  
    15.6 0.272 0.274 0.273 0.143  
    7.8 0.204 0.215 0.210 0.080  
    0 0.131 0.129 0.130    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

 常見問題解答
Q:

Can you run this assay on samples (whole blood or serum) that has been previously frozen or do samples have to be fresh?

A:
Thanks for your inquiry.
CSB-E09233h
This kit can test serum and plasma samples( not whole blood). It's better to test fresh samples;
If the sample has been previously frozen, pls pay attention to the storage time and avoid repeat freezing and thawing. Repeat freezing and thawing will destroy the structure of protein and affects detection. We suggest you do a pretest first if you want to test frozen sample.


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