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Human colon cancer-specific antigen-2,CCSA-2 ELISA kit

  • 中文名稱:
    人大腸癌專一抗原2(CCSA-2)酶聯免疫試劑盒
  • 貨號:
    CSB-E11291h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人大腸癌專一抗原2(CCSA-2)酶聯免疫試劑盒(CSB-E11291h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CCSA-2含量。試劑盒檢測范圍為3.33 ng/mL-80 ng/mL,適用于大腸癌發病機制研究、抗癌藥物體外篩選、治療效果評估及疾病動物模型中CCSA - 2動態變化分析。適用于實驗室條件下探索CCSA - 2在大腸癌微環境調控、腫瘤侵襲性評價等領域的生物學功能。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    CCSA-2
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    3.33 ng/mL-80 ng/mL
  • 靈敏度:
    1.33 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<10%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %92
    Range %88-100
    1:4Average %98
    Range %92-106
    1:8Average %95
    Range %86-101
  • 回收率:
    The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-102
    EDTA plasma (n=4)9590-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.015 1.970 1.993 1.946
    401.344 1.358 1.351 1.304
    200.799 0.801 0.800 0.753
    6.670.341 0.359 0.350 0.303
    3.330.201 0.215 0.208 0.161
    00.047 0.047 0.047  
  • 數據處理:
  • 貨期:
    3-5 working days

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