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Human cluster of differentiation 34(CD34) ELISA Kit

  • 中文名稱:
    人CD34分子酶聯免疫試劑盒
  • 貨號:
    CSB-E14091h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人CD34分子酶聯免疫試劑盒(CSB-E14091h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CD34含量。CD34是一種細胞表面糖蛋白,在造血干細胞、血管內皮祖細胞等表達。其背景在于對造血系統研究有重要意義。研究機制上,它可作為識別標記用于分離和富集造血干細胞,還參與細胞黏附、遷移等過程,助力造血調控的研究。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于體外研究中CD34蛋白水平的定量分析,如干細胞分化調控實驗、血管生成模型評估、血液系統疾病機制探索等基礎科研場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CD34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %88
    Range %82-92
    1:2Average %100
    Range %96-103
    1:4Average %100
    Range %97-105
    1:8Average %94
    Range %86-98
  • 回收率:
    The recovery of human CD34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-96
    EDTA plasma (n=4)9693-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.441 2.478 2.460 2.353
    101.876 1.912 1.894 1.787
    51.244 1.256 1.250 1.143
    2.50.689 0.707 0.698 0.591
    1.250.424 0.448 0.436 0.329
    0.6250.323 0.311 0.317 0.210
    0.3120.202 0.189 0.196 0.089
    00.109 0.105 0.107  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 基因功能參考文獻:
    1. This study aimed to confirm and analyze lymphovascular invasion (LVI) detected by hematoxylin and eosin (H and E) using IHC with CD34 and D2-40 and its correlation with other biologic and morphologic prognostic markers. D2-40 is a promising marker for lymphatic endothelium. LVI is a poor prognostic marker hence should be evaluated imperatively in all cases of carcinoma breast. PMID: 29567882
    2. The role of CD34 in determining the premalignant nature of ral submucous fibrosis (OSF) could not be ascertained, since all endothelial cells were positive for CD34, whereas CD105 appeared to be more specific as it is associated with hypoxia-induced angiogenesis which is occurring in OSF due to hyalinization suggesting, CD105 to be more specific marker to determine neoangiogenesis in OSF PMID: 30197335
    3. EZH2, ET-1, and CD34 may act as biomarkers of aggressive cervical squamous cell carcinoma PMID: 29630085
    4. The effects of Platelet-rich plasma on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin PMID: 28948378
    5. Disruption of GFI1B non-DNA-binding zinc-finger 1 is associated with mild to moderate thrombocytopenia without alpha-granule deficiency or bleeding symptomatology, indicating that the site of GFI1B mutation has important phenotypic implications. Platelet CD34 expression appears to be a common feature of perturbed GFI1B function. PMID: 28880435
    6. The endometrial expression of LIF and CD34 in the pathogenesis of non-developing pregnancy can be used for evaluating the pregnancy prognosis in women of young and old reproductive age. PMID: 29063331
    7. In summary, this study shows that CD34 plays a role during cell cycle progression of head and neck squamous cell carcinoma and additionally is involved in irradiation resistance and differentiation of malignant oral keratinocytes. PMID: 27837456
    8. Report CD34 expression in dermatofibromas and dermatofibrosarcoma protuberans. PMID: 28832078
    9. CD34+ clusters showed more important prognostic impact on survival and progression to leukaemia. PMID: 28669578
    10. Our findings showed that increased expression of CD34 and CD117 markers confer tumor progression and aggressiveness on prostate cancer. PMID: 28552539
    11. CD34(+) and CD34(-) cells are different phenotypes of similar cells, and CD34 (1) can be regulated in endothelial colony-forming cells ECFC; (2) is positively involved in capillary-like sprout formation; (3) is associated but not causally related to tip-cell gene expression; and (4) can affect endothelial barrier function. PMID: 27043316
    12. Studied levels of peripheral blood CD34(+ )cells in women who subsequently had a spontaneous miscarriage (SM). Found CD34 positive B cells were significantly lower in SM group compared to control group. PMID: 28110587
    13. The number of platelets and circulating CD34-positive cells acts as an indicator of the activity of the vicious cycle that exists between hypertension and endothelial dysfunction in elderly Japanese men. PMID: 28282559
    14. ESAT-6, an essential virulence factor of Mycobacterium tuberculosis, may contribute to the abnormal hematopoiesis of tuberculosis patients by inhibiting the proliferation and differentiation of CD34-positive hematopoietic cells via apoptosis. PMID: 27745787
    15. Intramyocardial autologous CD34(+) cell administration improved exercise tolerance in patients with refractory angina. PMID: 27491607
    16. Increased gene expression linked to the proinflammatory TGFbeta and MAPK signaling pathways were detected in CD34(+) cells of myeloproliferative neoplasm patients. PMID: 27341002
    17. CD34 stem cell exosomes mediate ischemic tissue repair via beneficial angiogenesis. Exosome-shuttled proangiogenic miRNAs may amplify stem cell function. PMID: 28298297
    18. miR-378 regulates the angiogenic capacity of CD34(+) progenitor cells in STEMI patients. PMID: 28062497
    19. CD34 and IL-8 are associated with poor prognosis of melanoma, metastasis, and neoangiogenesis PMID: 27175552
    20. The results showed that transfection of CD34(+) cells with SiRNA targeting TGF-bRII and their co-culture with human bone marrow mesenchymal stromal cells (MSCs) could considerably increase the number of progenitors PMID: 27344285
    21. Expression of CD34 and MMP-9 accurately predicts clinical behavior detection and prognosis of GCTBs. PMID: 27384731
    22. Expression of VEGF and CD34 was critically correlated with perioperative hemorrhage in gastric cancer patients. PMID: 27503021
    23. There was significant correlation between age and tumor size with CD34 expression in oral SCC samples and no significant correlation between sex and tumor differentiation level. Also, there was no significant correlation between age, sex, tumor size and tumor differentiation level (grading) with CD34 expression in esophagus SCC samples. PMID: 26323448
    24. Normal FLT3 and negative expression of CD34 and cMPL may predict a longer overall survival in aute myeloid leukemia. PMID: 27993871
    25. High VEGF expression was subsequently correlated with a short overall survival rate for patients exhibiting lymph node metastasis (P=0.0128); however, there was no significant difference in overall survival rate regarding the expression levels of TP and CD34 PMID: 26676225
    26. Metformin simultaneously increases VEGFA and reduces CXCL10 and TIMP1 in CD34(+) cells in a model of the diabetic state combined with hypoxia to stimulate angiogenesis. PMID: 26861446
    27. Immunohistochemistry results indicate that combination with ALDH1 and STAT6 can improve the diagnostic value of CD34 for solitary fibrous tumors. PMID: 26617768
    28. CD34(+) cells lowering expression of TET2 may play an oncogenic role on myeloid tumor and CD3(+) T cells of myelodysplastic syndrome patients may be derived from the malignant clone. PMID: 26617797
    29. ICAM-1, VCAM-1 and CD34 are useful biomarkers in evaluation of vascular and inflammatory lesions such as gingival pyogenic granuloma and the results indicate the role of these biomarkers in pathogenesis of oral pyogenic granuloma. PMID: 26625754
    30. Studied the relationship between serum alkaline phosphatase, circulating CD34-positive cells and body mass index. PMID: 26769093
    31. Autophagy in CD34+ cells from aplastic anemia patients is impaired; autophagy is required for the survival of CD34+ cells; impaired autophagy in CD34+ hematopoietic progenitor cells may play an important role in the pathogenesis of aplastic anemia. PMID: 26930650
    32. overexpression in glioma tissues associated closely with higher WHO grade (III + IV) [review] PMID: 26886640
    33. Asthmatics with reduced lung function had a higher frequency of Lin(-) CD34(hi) CD117(int/hi) Fc epsilon RI(+) blood mast cell progenitors than asthmatics with normal lung function. PMID: 26626992
    34. Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
    35. Data indicate only the glycoproteins CD34+/CD71low population showed statistically significant differences between Diamond-Blackfan Anaemia (DBA) patients and controls. PMID: 26394034
    36. We here identify CD49f and CD34 as markers to permit selection of the stem/progenitor cell-like population from human myometrium PMID: 26108791
    37. CD34 expression in the placental endothelial cells was stable throughout pregnancy. PMID: 25043745
    38. Autologous CD 34(+) cell infusion is safe and effectively improves liver function in the short term and may serve as a bridge to liver transplantation. PMID: 26109814
    39. Higher percentages of CD34 + CD38- cells in apheresis products reflect inadequate in vivo purging and reliably distinguish samples enriched in LSCs from those involving mainly normal cells. PMID: 24884314
    40. The number of CD34/144 positive circulating endothelial cells was high in patients with large artery atherosclerosis who experienced chronic ischemic stroke. And this number may reflect severity of carotid atherosclerosis. PMID: 25542764
    41. The evaluation of both CD34-CD105 showed the role of angiogenesis in the cancer proliferation and local spread, the angiogenesis level being maintained high even in the advanced stages of the disease. PMID: 26193208
    42. There were significant differences in CD34 expression between papillary thyroid hyperplasia, papillary thyroid carcinoma, and papillary thyroid lesions of uncertain malignant potential. PMID: 25921136
    43. lack of predictive factors for CD34(+) cells and total nucleated cell concentrations in umbilical cord blood samples before processing PMID: 25446160
    44. The number of CD34(+) cells in bone marrow was significantly higher compared to the number of CD34(+) cells in peripheral blood cells and increased with the acquisition of leukemic cells the resistance to imatinib. PMID: 25804236
    45. CD34 and CD49b are cell surface markers that can be used to enrich a subpopulation of leiomyoma cells possessing stem/progenitor cell properties. PMID: 25658015
    46. no difference in expression between normal and pathologically immature placentas PMID: 25149387
    47. Report CD34/MMP7 expression and invasiveness in early colorectal neoplasms using narrow band imaging and immunohistochemistry. PMID: 25357219
    48. Angiogenic factors VEGF, HIF-1alpha and CD34 are important in breast cancer progression and their abundance in breast tumors has prognostic and predictive value. PMID: 23899963
    49. Combinatorial expression of NKX2-5, SIRPA, VCAM1 and CD34 can be used to define discrete stages of cardiovascular cell lineage differentiation. PMID: 24968096
    50. In MLL-AF4 patients, CD34(+)CD38(+)CD19(+) and CD34(-)CD19(+) cells initiated leukemia. PMID: 25538041

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  • 亞細胞定位:
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    CD34 family
  • 組織特異性:
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 數據庫鏈接:

    HGNC: 1662

    OMIM: 142230

    KEGG: hsa:947

    STRING: 9606.ENSP00000310036

    UniGene: Hs.374990



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