Human cholecystokinin octapeptide,CCK-8 ELISA Kit
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中文名稱:人膽囊收縮素/縮膽囊素八肽(CCK-8)酶聯免疫試劑盒
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貨號:CSB-E08986h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人膽囊收縮素/縮膽囊素八肽(CCK-8)酶聯免疫試劑盒(CSB-E08986h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的CCK-8含量。CCK-8即細胞計數試劑盒-8,是常用的檢測細胞增殖和細胞毒性的試劑。其背景源于對高效便捷細胞活性檢測方法的需求。研究機制是基于WST-8在電子載體作用下被細胞線粒體中的脫氫酶還原為具有高度水溶性的甲臜產物,通過測定吸光度反映細胞活性。試劑盒檢測范圍為0.57 ng/mL-10 ng/mL,適用于胃腸動力學研究、神經內分泌調控機制探索、代謝性疾病動物模型評價等科研領域。可滿足基礎研究中對CCK - 8動態監測的需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:CCK-8
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma
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檢測范圍:0.57 ng/mL-10 ng/mL
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靈敏度:0.357 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 85-97 1:2 Average % 96 Range % 90-106 1:4 Average % 95 Range % 86-104 1:8 Average % 93 Range % 88-96 -
回收率:
The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 93-108 EDTA plasma (n=4) 95 89-103 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 0.57 0.048 0.051 0.050 1.14 0.162 0.153 0.158 2.29 0.376 0.363 0.370 5.71 0.838 0.842 0.840 10 1.655 1.667 1.661 -
數據處理:
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貨期:3-5 working days
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