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Human cholecystokinin,CCK ELISA Kit

  • 中文名稱:
    人膽囊收縮素/腸促胰酶肽(CCK)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08116h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人膽囊收縮素/腸促胰酶肽(CCK)酶聯(lián)免疫試劑盒(CSB-E08116h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CCK含量。CCK即膽囊收縮素,在生理上可調(diào)節(jié)膽囊收縮、胃腸運(yùn)動(dòng)等。研究機(jī)制方面,它通過與相關(guān)受體結(jié)合發(fā)揮作用,在神經(jīng)系統(tǒng)和消化系統(tǒng)中參與多種生理功能調(diào)節(jié),其信號通路的研究對理解神經(jīng)胃腸病等疾病發(fā)病機(jī)制有重要意義。試劑盒檢測范圍為56 pg/mL-800 pg/mL,該產(chǎn)品可應(yīng)用于胃腸功能研究、攝食行為神經(jīng)機(jī)制探索、代謝綜合征模型評估等科研領(lǐng)域,特別適合需要精確測定生物體液或組織樣本中CCK動(dòng)態(tài)變化的實(shí)驗(yàn)設(shè)計(jì),其寬檢測范圍可滿足基礎(chǔ)研究中正常生理水平到病理狀態(tài)下的濃度檢測需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    (1-49)-CCK58 ELISA Kit; CCK ELISA Kit; CCK12 ELISA Kit; CCK18 ELISA Kit; CCK25 ELISA Kit; CCK33 ELISA Kit; CCK39 ELISA Kit; CCK5 ELISA Kit; CCK58 ELISA Kit; CCK7 ELISA Kit; CCK8 ELISA Kit; CCKN_HUMAN ELISA Kit; Cholecystokinin-5 ELISA Kit
  • 縮寫:
    CCK
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    56 pg/mL-800 pg/mL
  • 靈敏度:
    20 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    80-98

    1:2

    Average %

    95

    Range %

    91-105

    1:4

    Average %

    87

    Range %

    82-94

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of human CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    93

    90-100

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    800

    2.453

    2.504

    2.478

    2.478

    400

    1.275

    1.287

    1.281

    1.281

    280

    0.756

    0.748

    0.752

    0.752

    144

    0.395

    0.410

    0.402

    0.402

    56

    0.200

    0.205

    0.203

    0.203

    0

    0.112

    0.113

    0.113

    0.113

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

 常見問題解答
Q:

I?would?like?to?ask?you?if?you?know?what?is?the?most?common?sample?dilution?in?human?CCK?elisa?kit?

A:
Thanks for your inquiry.
CSB-E08116h, generally there is no need to dilute when detecting.
And if you arrange the preliminary test and find the sample needs dilution, pls consider to use the PBS.

靶點(diǎn)詳情

  • 功能:
    This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • 基因功能參考文獻(xiàn):
    1. We found the suicide-associated gene coexpression network. The reconstructed network consisted of 104 genes. Topological analysis showed that in total, CCK, INPP1, DDC, and NPY genes are the most fundamental hubs in the network PMID: 29381655
    2. L-trp is a luminal regulator of CCK release with effects on gastric emptying, an effect that could be mediated by CCK. L-trp's effect on GLP-1 secretion is only minor. At the doses given, the two amino acids did not affect subjective appetite feelings. PMID: 27875537
    3. The CCK polymorphism have reported significant association of -45C>T polymorphism with the presence of hallucinations. PMID: 27084212
    4. CCK does not appear to play a unique independent role in satiety/satiation. PMID: 26429068
    5. CCK release has been found to be halved in pregnant women with hyperemesis gravidarium, which supports the hypothesis that gastrointestinal motility is increased in pregnant women with hyperemesis gravidarium. PMID: 25331205
    6. CCK in plasma is an independent marker of cardiovascular mortality in elderly female patients. PMID: 26878472
    7. These data offer preliminary evidence supporting an association between the rs1799923 polymorphism in the CCK gene and PTSD PMID: 26454231
    8. Data suggest that endocrine responses differ between jejunal and gastric enteral feeding, with higher peak plasma CCK (cholecystokinin), PYY (peptide YY), and GLP-1/2 (glucagon-like peptides 1/2) concentrations being attained after jejunal feeding. PMID: 26762368
    9. active GLP-1 produced in the islet stimulates cholecystokinin production and secretion in a paracrine manner via cyclic AMP and CREB. PMID: 25984632
    10. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. Plasma Pro-CCK is a prognostic marker in patients with stable heart failure. PMID: 25627687
    11. CCK binding modulates the contractile function of the lower esophageal sphincter through differential binding to the CCK-A receptor on the sling and clasp fibers PMID: 24914377
    12. Data suggest that up-regulation of plasma CCK levels is 50% higher following breakfast of meal replacement beverage containing fat emulsion of rapeseed oil with droplet size of 0.1 um (versus 0.3 um); satiety response and food intake are unaffected. PMID: 23975326
    13. These studies identify a key role for Cck in the development and treatment of mania. PMID: 23399917
    14. Results suggest excessive local release of CCK in response to duodenal lipid in functional dyspepsia. PMID: 24252886
    15. CCK enhances cholesterol absorption by activation of a pathway involving CCK1R/CCK2R, Gbetagamma, PI3K, Akt, Rab11a, and NPC1L. PMID: 24692543
    16. This review will summarize what is known regarding CCK, its receptors, and pancreatic--{REVIEW} PMID: 24177032
    17. We aimed to determine whether fasting or meal-stimulated ghrelin, PYY, CCK, and satiety responses are different between lean PCOS patients and healthy women. PMID: 24001751
    18. hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
    19. Normal CCK profiles in AN at admission indicates hormonal responses adapted to low food intake while change of eating habits and weight gain PMID: 23349895
    20. CD36 is a major mediator of FA-induced release of CCK and secretin. These peptides contribute to the role of CD36 in fat absorption and to its pleiotropic metabolic effects. PMID: 23233532
    21. a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin PMID: 23064014
    22. CCK remarkably increases the firing frequency of action potentials of layer III pyramidal neurons in the entorhinal cortex. PMID: 21753024
    23. CCK gene -45C/T locus T allele was positively associated with schizophrenia in female Han population in northern China. PMID: 21542221
    24. it is likely that unsulfated CCK peptides constitute a separate hormone system that acts via CCK-B receptors. [review] PMID: 21985915
    25. This article reviews the role of cholecystokinin (CCK), a satiety-producing hormone, in the regulation of binge eating in those who suffer from bulimia nervosa [review] PMID: 22188045
    26. The results of this study demonistrated that the cholecystokinin polymorphisms is related toin Parkinson's disease hallucinations in asian group. PMID: 21506165
    27. CCK modulates intrinsic neuronal excitability and synaptic transmission in a surprisingly cell-type specific manner, acting as a key molecular switch to regulate the functional output of neuronal circuits. PMID: 21154912
    28. Cholecystokinin gene polymorphisms are not associated with antipsychotic induced weight gain in schizophrenia patients. PMID: 20732371
    29. Neither treatment of pancreatic cancer cells with CCK antibodies nor the down-regulation of CCK mRNA and peptide by shRNAs altered growth in vitro or in vivo. PMID: 21186400
    30. These results point to a potential route of action by which components of Hoodia might influence appetite control; the natural taste receptor antagonist was identified for further studies as an appetite suppressant. PMID: 20930049
    31. Study compared CCK-like immunoreactivity (CCK-LI) in plasma from 25 subjects in the late luteal phase (LLP) and the follicular phase (FP) and found that there was no difference during the menstrual cycle PMID: 20457200
    32. results suggest that the CCK system may play a role in the pathogenesis of panic disorder, with susceptibility alleles both protecting and contributing to the disease PMID: 20023595
    33. REVIEW: role in thermoregulation and other aspects of energetics PMID: 20036221
    34. Cefaclor, when given before a meal in the form of a capsule, does not stimulate CCK release or slow gastric emptying in healthy humans. PMID: 19914303
    35. This article reviews the corpus of work supporting the role of CCK in anxiety and suggests three research approaches which can further enhance our understanding of the CCK-2 system in panic disorder. PMID: 11713976
    36. This review describes the signaling pathways and transcription factors involved in neuronal CCK gene transcription. PMID: 11713982
    37. Endoproteolytic processing events of heterologously expressed cholecystokinin are studied in Saccharomyces cerevisiae. PMID: 11713985
    38. mapping of the CCK1R binding site by identifying residues that interact with the methionine and phenylalanine residues of the C-terminal moiety of CCK PMID: 11724786
    39. Deteriorating gallbladder contractions, possibly induced by alterations in the CCK-AR gene, as well as CCK-AR gene polymorphism, promoted gallstone formation. PMID: 12572876
    40. Results suggest that the L-(-188G) haplotype may act as a protective factor against panic by reducing the expression of anxiogenic cholecystokinin (CCK). PMID: 12627463
    41. in Chinese, visual hallucinations in Parkinson's disease are associated with cholecystokinin -45C>T polymorphism, and this association was still observed in the presence of the cholecystokinin-A receptor TC/CC genotype PMID: 12777967
    42. reduced basal hunger, rather than increased meal-induced satiety, contributes to the anorexia of aging and that changes in cholecystokinin are unlikely to be responsible PMID: 12915664
    43. CCK's suppression of food intake is enhanced when the stomach is distended. PMID: 12920059
    44. Ingestion of a CCK-releasing fatty acid reduces the tolerated volume of liquid delivered into the stomach, primarily via a CCK(1) receptor-mediated delay in gastric emptying. PMID: 14764444
    45. Review summarizes the physiological role of CCK not only in the stimulation of pancreatic and biliary secretions but also in the regulation of gastrointestinal motility. PMID: 15100163
    46. Study based on a small, well-characterized matched case-control group of patients with Parkinson disease (PD) suggests that the cholecystokinin system may influence the development of hallucinations in PD subjects. PMID: 15313848
    47. Ewing sarcomas synthesize and secrete proCCK that can be identified in plasma as circulating tumor marker. PMID: 15328192
    48. CCK regulates pancreatic enzyme secretion and growth, intestinal motility, satiety signalling and the inhibition of gastric acid secretion--REVIEW PMID: 15533776
    49. conclude that women with polycystic ovary syndrome (PCOS) have reduced postprandial cholecystokinin(CCK) secretion and deranged appetite regulation associated with increased levels of testosterone PMID: 15624269
    50. Gastrin and CCK exert a trophic action on some of the biliary tract cancers. PMID: 15682471

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Gastrin/cholecystokinin family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 1569

    OMIM: 118440

    KEGG: hsa:885

    STRING: 9606.ENSP00000335657

    UniGene: Hs.458426



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