在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human chaperonin 10,CPN10 ELISA Kit

  • 中文名稱:
    人伴侶蛋白10(CPN10)酶聯免疫試劑盒
  • 貨號:
    CSB-E09917h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人伴侶蛋白10(CPN10)酶聯免疫試劑盒(CSB-E09917h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HSPE1含量。HSPE1是一個值得關注的靶點。它在細胞生理活動中發揮重要作用,與細胞應激反應、蛋白質折疊等相關。研究機制主要聚焦于其在疾病發生發展中的功能,如在腫瘤細胞增殖、存活等過程的作用,通過調控它或能找到新治療策略。試劑盒檢測范圍為0.16 ng/mL-10 ng/mL,適用于體外檢測CPN10在細胞應激機制研究、疾病模型(如神經退行性疾病或代謝綜合征)中的動態表達分析,以及蛋白質相互作用的基礎科研領域,為探索CPN10生物學功能提供可靠的實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    10 kDa chaperonin ELISA Kit; 10 kDa heat shock protein mitochondrial ELISA Kit; 10 kDa heat shock protein; mitochondrial ELISA Kit; CH10_HUMAN ELISA Kit; Chaperonin 10 ELISA Kit; Chaperonin 10 homolog ELISA Kit; CPN10 ELISA Kit; cpn10 homolog ELISA Kit; Early pregnancy factor ELISA Kit; Early-pregnancy factor ELISA Kit; EPF ELISA Kit; GROES ELISA Kit; GroES homolog ELISA Kit; Heat shock 10kD protein 1 chaperonin 10 ELISA Kit; Heat shock 10kDa protein 1 ELISA Kit; Heat shock 10kDa protein 1 chaperonin 10 ELISA Kit; Heat shock protein family E (Hsp10) member ELISA Kit; Heat-shock 10-kD protein ELISA Kit; Hsp10 ELISA Kit; Hspe1 ELISA Kit
  • 縮寫:
    HSPE1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.16 ng/mL-10 ng/mL
  • 靈敏度:
    0.04 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-94
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 94
    Range % 87-98
    1:8 Average % 102
    Range % 98-106
  • 回收率:
    The recovery of human CPN10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 83-94
    EDTA plasma (n=4) 92 87-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.688 2.579 2.634 2.525
    5 2.154 2.054 2.104 1.995
    2.5 1.365 1.302 1.334 1.225
    1.25 0.742 0.734 0.738 0.629
    0.63 0.417 0.405 0.411 0.302
    0.31 0.274 0.266 0.270 0.161
    0.16 0.186 0.183 0.185 0.076
    0 0.110 0.108 0.109  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.
  • 基因功能參考文獻:
    1. elevated expression of HSP10 protein inhibits apoptosis and associates with poor prognosis of astrocytoma PMID: 29028811
    2. miR-146a, miR-146b, and miR-155 are exerting anti-inflammatory properties by down-regulating IL-6 and IL-8, and influencing the expression of HSP10 in the activated endothelium PMID: 28662100
    3. High expression of HSP10 is negatively associated with estrogen receptor/progesterone receptor status and might be a novel independent biomarker for poor prognosis in invasive ductal breast carcinoma. PMID: 27993580
    4. EPF induces the differentiation of regulatory T cells and increases their immunosuppressive activities. PMID: 27840373
    5. Cpn10 has a role in the spatial regulation of NPAT signaling PMID: 26429916
    6. Hsp10 has a role in nuclear localization and lung cells response to cigarette smoke PMID: 25355063
    7. Data show that that in presence of 300 mg/mL Ficoll the thermodynamic stability of each cpn10 monomer increases by over 30%, whereas the interfaces are stabilized by less than 10%. PMID: 21375247
    8. HSP10 protein was detected only in oocytes from human preantral follicles, whereas in antral follicles, it was localised in oocytes, granulosa cells, theca cells and stroma cells. PMID: 19903031
    9. Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. PMID: 11898127
    10. The low stability of the monomeric unit suggests that folding and assembly reactions for cpn10 are coupled. PMID: 12220543
    11. Cpn10 and placental lactogen are capable of stimulating the synthesis of type I collagen by human osteoblasts in culture PMID: 12703979
    12. Identification of amino acids important for the assembly of the cpn10 heptamer. PMID: 14525625
    13. complex mechanisms are involved in the protection by hsp10 against simulated ischemia and reoxygenation-induced myocyte death PMID: 15059967
    14. The HSP10 plays a role in bone marrow cell differentiation other than being a mitochondrial co-chaperonin. PMID: 15590416
    15. Chaperonin 10 and calgranulin A are identified as markers for diagnosis and screening of endometrial carcinoma. PMID: 15816004
    16. the cpn10 interfaces can adapt to structural alterations without loss of either subunit-subunit affinity or heptamer specificity PMID: 15978542
    17. biophysical analysis of dissociation equilibrium for the heptameric co-chaperonin proteins 10 from Aquifex aeolicus and human mitochondria PMID: 16100270
    18. proteomic analysis of possible role of heat shock protein 10 in colorectal cancer PMID: 16502466
    19. Results describe the time-resolved folding and assembly mechanism of the heptameric co-chaperonin protein 10 (cpn10) in vitro. PMID: 16979655
    20. Investigation of single-nucleotide variations in the Hsp10 gene and their disease-causing potential. PMID: 17072495
    21. In this review, we revise the involvement of Hsp10 in signal transduction pathways and its possible role in cancer etiology. PMID: 17278877
    22. Data show that Hhsp10 formed fibrils from only the acidic unfolded state and Core peptide regions of these protein fibrils were determined by proteolysis followed by a combination of Edman degradation and mass spectroscopy analyses. PMID: 18329043
    23. The effects of cpn 10 on cells of the oligodendrocyte lineage, were assessed. PMID: 18465204
    24. In patients with serous ovarian carcinomas, gene expression analysis coupled with immunohistochemistry allowed the identification of HSP10 as an independent factor of progression-free survival. PMID: 18500265
    25. Results describe a novel function of chaperonin 10 as a general differentiation factor, not limited to erythroid cells, and show how this biological effect is mediated by GSK-3alpha/beta. PMID: 19142874
    26. HSP10 was identified as a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19520060

    顯示更多

    收起更多

  • 亞細胞定位:
    Mitochondrion matrix.
  • 蛋白家族:
    GroES chaperonin family
  • 數據庫鏈接:

    HGNC: 5269

    OMIM: 600141

    KEGG: hsa:3336

    STRING: 9606.ENSP00000233893

    UniGene: Hs.1197



主站蜘蛛池模板: 亚洲精品久久夜色撩人男男小说| 日韩成人无码v清免费| 日韩高清在线观看永久| 精品无码一区二区三区av| 亚洲国产精品无码一线岛国| 国产高清japanese在线播放e| 老汉色老汉首页a亚洲| 欧洲精品一线二线三线区别| 精品深夜av无码一区二区| 中国熟妇浓毛hdsex| 日本成本人三级在线观看| 亚洲人成人无码www影院 | 成年免费视频黄网站zxgk| 国产av一区二区三区天堂综合网| 中文字字幕在线乱码视频| 亚洲国产精品毛片av不卡在线| 欧美激情第1页| 国产a精彩视频精品视频下载 | 人妻av乱片av出轨av| 亚洲中文字幕无码中文字在线 | 免费福利视频一区二区三区高清| 亚洲色欲综合一区二区三区| 51久久成人国产精品麻豆| 动漫精品无码h在线观看| 国产无遮挡又黄又爽网站| 性生交大片免费看| 女人喷水高潮时的视频网站| 欧美极品色午夜在线视频| 国产精品日韩欧美一区二区三区| 另类老妇奶性生bbwbbw| 国产精品高潮露脸在线观看| 免费看男女做羞羞的事网站| 免费人妻av无码专区| 四虎影视精品永久在线观看| 2018高清国产一区二区三区| 中文成人无字幕乱码精品区| 亚洲国产成人片在线观看无码| 日本午夜免a费看大片中文4| 国产精品无码天天爽视频| 久久国产劲爆∧v内射-百度| 欧洲日本一线二线三线区本庄铃 |