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Human carbohydrate-deficient transferrin,CDT ELISA Kit

  • 中文名稱:
    人糖缺失性轉鐵蛋白(CDT)酶聯免疫試劑盒
  • 貨號:
    CSB-E09602h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人糖缺失性轉鐵蛋白(CDT)酶聯免疫試劑盒(CSB-E09602h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CDT含量。試劑盒檢測范圍為50 ng/mL-1600 ng/mL,適用于科研場景中評估酒精攝入對轉鐵蛋白糖基化修飾的影響,探究慢性酒精暴露導致的肝臟代謝異常機制,或作為酒精相關疾病動物模型的研究工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    CDT
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    50 ng/mL-1600 ng/mL
  • 靈敏度:
    25 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 93-105  
    1:2 Average % 86  
    Range % 82-94  
    1:4 Average % 96  
    Range % 92-100  
    1:8 Average % 88  
    Range % 82-96  
  • 回收率:
    The recovery of human CDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-102  
    EDTA plasma (n=4) 87 82-93  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1600 1.849 1.951 1.900 1.865  
    800 0.983 0.999 0.991 0.956  
    350 0.476 0.457 0.467 0.432  
    150 0.264 0.247 0.256 0.221  
    50 0.104 0.108 0.106 0.071  
    0 0.034 0.036 0.035    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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