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中文名稱:人的牛血清白蛋白(BSA)酶聯免疫試劑盒
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貨號:CSB-E08633h
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規格:96T/48T
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價格:¥3900/¥2500
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其他:
產品詳情
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產品描述:人的牛血清白蛋白(BSA)酶聯免疫試劑盒(CSB-E08633h)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的BSA含量。BSA即牛血清白蛋白,背景上,它來源豐富、價格低廉,在生物醫學領域廣泛應用。研究機制方面,其可作為載體蛋白,結合藥物、小分子等;也能模擬體內血漿環境,用于藥物體外研究,還可用于酶活性測定、免疫分析等。試劑盒檢測范圍為0.312 μg/mL-80 μg/mL,靈敏度為0.312 μg/mL。本試劑盒通過預包被特異性抗體,利用樣本中BSA與酶標記BSA競爭結合抗體的特性,結合顯色反應實現定量分析,可廣泛用于實驗室評估樣本制備過程中BSA的添加濃度、檢測實驗體系中的外源性BSA干擾,或作為生物制品生產過程中蛋白成分的質控工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:BSA
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma
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檢測范圍:0.312 μg/mL-80 μg/mL
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靈敏度:0.312 μg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 80-92 1:2 Average % 95 Range % 91-105 1:4 Average % 95 Range % 92-100 1:8 Average % 93 Range % 85-98 -
回收率:
The recovery of human BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-108 EDTA plasma (n=4) 97 90-110 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 80 0.286 0.291 0.289 20 0.435 0.442 0.439 5 0.589 0.593 0.591 1.25 0.799 0.805 0.802 0.312 1.228 1.237 1.233 0 1.772 1.782 1.777 -
數據處理:
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貨期:3-5 working days
引用文獻
- An electrochemical aptasensing platform for carbohydrate antigen 125 based on the use of flower-like gold nanostructures and target-triggered strand displacement amplification Chen J,Microchimica Acta,2019
- Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification Qingzhen Zhou.et al,Royal Society of Chemistry,2018
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