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  4. Human blca-4 ELISA Kit

Human blca-4 ELISA Kit

  • 中文名稱：
    人blca-4酶聯免疫試劑盒
  • 貨號：
    CSB-E14959h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人blca-4酶聯免疫試劑盒（CSB-E14959h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的BLCA-4含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領域中對膀胱癌發病機制、腫瘤微環境調控及生物標志物篩查的研究，支持多種生物樣本類型的平行檢測，為體外實驗提供穩定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    BLCA-4
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 84-91  
    1:2 Average % 99  
    Range % 96-103  
    1:4 Average % 104  
    Range % 101-107  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 84-90  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.595 2.540 2.568 2.452  
    20 2.208 2.365 2.287 2.171  
    10 1.823 1.804 1.814 1.698  
    5 1.195 1.175 1.185 1.069  
    2.5 0.698 0.678 0.688 0.572  
    1.25 0.418 0.432 0.425 0.309  
    0.625 0.271 0.276 0.274 0.158  
    0 0.115 0.117 0.116    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

I am interested in purchasing the kit for a study that we wanted to perform. Can you please tell me more abou the antibodies that comprise this kit?

A:
Thanks for your inquiry.
CSB-E14959h The kit uses double antibody sandwich method to quatitative detect blca-4.
And its coated antibody is goat polyclonal antibody, biotin-conjugated antibody (that is, detection antibody) is goat polyclonal antibody
As for the specific components of the kit can be seen in the manual MATERIALS PROVIDED part.I will send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.


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