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Human anti-Mullerian hormone (AMH) ELISA kit

  • 中文名稱:
    人繆勒管抑制物質(zhì)/抗繆勒管激素(AMH)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12756h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人繆勒管抑制物質(zhì)/抗繆勒管激素(AMH)酶聯(lián)免疫試劑盒(CSB-E12756h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的AMH含量。抗繆勒管激素(AMH)在生殖領(lǐng)域背景重要,它由卵巢顆粒細(xì)胞分泌,可反映卵巢儲(chǔ)備功能。研究機(jī)制上,AMH通過(guò)與特定受體結(jié)合,參與卵泡發(fā)育的調(diào)節(jié),抑制原始卵泡募集及卵泡生長(zhǎng),為評(píng)估女性生育力等研究提供關(guān)鍵依據(jù)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL,靈敏度為15.6 pg/mL。適用于生殖生物學(xué)研究、內(nèi)分泌調(diào)控機(jī)制探索以及疾病模型中激素水平變化的評(píng)估。科研人員可通過(guò)分析卵巢儲(chǔ)備功能、睪丸支持細(xì)胞活性或相關(guān)動(dòng)物模型的性腺發(fā)育狀態(tài),為生殖系統(tǒng)疾病機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)等提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    AMH ELISA Kit; MIF ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    492.045

    500.047

    507.604

    498.579

    504.986

    493.245

    SD

    0.021

    0.035

    0.030

    0.045

    0.043

    0.039

    CV(%)

    3.477

    5.716

    4.792

    7.293

    6.902

    6.373

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    90

    Range %

    85-96

    1:2

    Average %

    96

    Range %

    90-108

    1:4

    Average %

    98

    Range %

    95-100

    1:8

    Average %

    97

    Range %

    90-105

  • 回收率:

    The recovery of human AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-101

    EDTA plasma (n=4)

    97

    90-105

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    4000

    2.656

    2.764

    2.710

    2.582

    2000

    1.710

    1.873

    1.792

    1.664

    1000

    1.096

    1.062

    1.079

    0.951

    500

    0.599

    0.609

    0.604

    0.476

    250

    0.373

    0.353

    0.363

    0.235

    125

    0.268

    0.264

    0.266

    0.138

    62.5

    0.204

    0.221

    0.213

    0.085

    0

    0.125

    0.131

    0.128

  • 本試劑盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AMH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled AMH antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

Could?I?ask,?is?this?product?a?generation?II?Elisa?kit???If?not,?could?you?suggest?an?alternative??

A:
Thanks for your inquiry.
You mentioned Generation II , it means anti-Mullerian hormone(AMH)generation II Elisa kit indeed.
And then it is this kit CSB-E12756h. Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    AMH是一種調(diào)節(jié)女性生殖系統(tǒng)發(fā)育和功能的蛋白質(zhì)。最近的研究發(fā)現(xiàn),AMH與代謝綜合征的發(fā)病有關(guān),代謝綜合征是一組包括高血壓、高血糖和肥胖等疾病的綜合癥。研究發(fā)現(xiàn),女性患有代謝綜合征的概率與AMH水平降低相關(guān)。這些發(fā)現(xiàn)表明,AMH可能是代謝綜合征的一個(gè)潛在生物標(biāo)志物,并為進(jìn)一步研究代謝綜合征的發(fā)病機(jī)制提供了新的思路。
  • 功能:
    This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
  • 基因功能參考文獻(xiàn):
    1. Women with higher testosterone and AMH had more frequent anovulatory cycles but there were marginal impacts on time to pregnancy or pregnancy loss. PMID: 29428315
    2. These findings indicate a pregnancy-associated AMH-decline independent of pre-pregnancy elevated AMH levels. PMID: 30146442
    3. serum AMH levels were negatively correlated with age. AMH concentrations approximately 31.1% depended on age and descended by an average of 6.2% per year. Around 25, 35 and 40 years, the decrease of AMH values accelerated. PMID: 29671678
    4. AMH is a poor independent predictor of live birth outcome in either fresh or frozen embryo transfer. PMID: 29331235
    5. AMH, IGF1 and leptin levels in follicular fluid have no relation to the fertility disorders caused by endometriosis or fallopian tube damage, though they are biomarkers for anovulatory fertility disorders. PMID: 29595066
    6. serum levels between women with insulin resistance and without insulin resistance in polycystic ovary syndrome were not significantly different PMID: 29605710
    7. Data suggest that serum levels of AMH are down-regulated during second half of gestation in (1) women with gestational diabetes, (2) pregnant women with type 2 diabetes, and (3) control pregnant women. A positive association between AMH and testosterone levels was observed in all groups. Also, serum AMH levels are negatively associated with maternal age in the population studied. PMID: 28758808
    8. Data confirm that serum levels of AMH decline as function of age (not menstrual cycle) in women ages 20-50; here, results of ELISA assays of serum levels of AMH vary according to kit used. These studies were conducted at a fertility clinic in Poland; serum AMH is proposed as biomarker for ovarian reserve. PMID: 28792788
    9. AMH single nucleotide polymorphism is associated with Endometriosis-associated infertility. PMID: 28831646
    10. This study demonstrated the existence of an AMH-FOXL2 relationship in hGCs. AMH is capable of increasing both gene and protein expression of FOXL2. Because FOXL2 induces AMH transcription, these ovarian factors could be finely regulated by a positive feedback loop mechanism to preserve the ovarian follicle reserve. PMID: 28660501
    11. Data suggest that circulating AMH influences development of human brain; high levels of circulating AMH are unique to developing boys; here, boys age 5-6 years exhibit up-regulation of circulating AMH but are delayed in maturity index (judged by drawings) as compared to girls age 5-6 years. This study was conducted in New Zealand. PMID: 28593614
    12. The combination of GDF9 + BMP15 potently stimulates AMH expression in primary cumulus cells. PMID: 28874516
    13. serum level elevated in polycystic ovary syndrome PMID: 27899014
    14. Surface plasmon resonance analysis showed no significant association between FS288 and AMHC , suggesting that FS288 indirectly regulates AMH signaling. Activin A, a direct target of FS288, did not itself induce reporter activity in P19 cells, but did prevent the FS288-induced increase in AMH signaling. Hence, local concentrations of FS288 and Activin A may influence the response of some cell types to AMH. PMID: 28500669
    15. The current study showed no significant difference in AMH level between Sudanese women with preeclampsia and the controls. AMH level was not associated with age, parity, gestational and body mass index. PMID: 28646929
    16. In a 46,XY phenotypically female patient bearing testes in inguinal canal, and diagnosed with complete androgen insensitivity syndrome, the dysfunctioning of AR by mutation enhanced AMH expression which ultimately leads to the failure in maturation of Sertoli cells. PMID: 28299491
    17. These findings underscore the importance of screening young females with AMH for possible occult primary ovarian insufficiency. PMID: 29176793
    18. Women with unexplained infertility did not show evidence of decreased ovarian reserve as measured by AMH and antral follicle count. PMID: 29202959
    19. An undetectable AMH level in women aged <40 at the end of chemotherapy for early stage breast cancer gave a good prediction that ovarian function would not return. PMID: 29117576
    20. In granulosa cells from women with PCOS, the regulation of AMH and AMHR2 expression is altered in a way that promotes the overexpression of the AMH/AMHR2 system, and could contribute to the follicular arrest observed in these patients. PMID: 28938480
    21. genetic variants of AMH or AMHR2 were not found to be associated with a higher risk for polycystic ovaries syndrome. PMID: 27664518
    22. Strong positive age-independent relationship between AMH level and the rate of euploid blastocysts I in vitro fertilization/intracytoplasmic sperm injection was found. PMID: 28987789
    23. The ratio of inactive proAMH precursor to receptor-competent AMHN,C differs in women with polycystic ovary syndrome relative to unaffected controls indicating that AMH signaling mechanisms may be altered in women with PCOS. PMID: 29079276
    24. Study found 24 rare AMH variants in patients with PCOS and control subjects; 18 variants were specific to women with PCOS. Seventeen of 18 (94%) PCOS-specific variants had significantly reduced AMH signaling, whereas none of 6 variants observed in control subjects showed significant defects in signaling. PMID: 28505284
    25. In the obese polycystic ovary syndrome group, anti-mullerian hormone was associated with ghrelin levels independent of age, insulin, and total testosterone. There was no association between total ghrelin and anti-mullerian hormone levels in non-obese women with polycystic ovary syndrome, non-obese controls, or obese controls. PMID: 28004236
    26. Data suggest that young men and young women initially have similar levels of circulating levels of AMH, but women lose their AMH in parallel with the decrease in their ovarian reserve. Circulating AMH in men is negatively associated with all-cause mortality; men with higher levels of AMH may outlive men with lower levels of AMH. [EDITORIAL] PMID: 27819112
    27. In young women with polycystic ovary syndrome, low AMH levels predict a greater risk of metabolic syndrome. PMID: 27842995
    28. MIS/AMH inhibits ovarian cancer by deregulating the Wnt signal pathway via the beta-catenin interacting protein (ICAT). MIS/AMH upregulated ICAT in ovarian cancer cell line which caused decreased cell viability, cell cycle arrest and apoptosis. PMID: 28197641
    29. The AMH level peaked at or before ovulation in most women, trended down with natural pregnancies, and consistently increased or decreased in women with a viable pregnancy after therapy. Nonviable pregnancies showed erratic AMH patterns. PMID: 27565260
    30. Before 35 years of age, women with type 1 diabetes have lower AMH levels than women without diabetes. PMID: 27475411
    31. Report variations in antimullerian hormone levels during the menstrual cycle. PMID: 27351446
    32. Suggest that activation of AMH by proteolytic enzymes is largely stable throughout the ovarian cycle. However, there is a subtle but robust decrease in the level of proAMH relative to AMHN,C in the acute postovulatory period. PMID: 27362611
    33. Age is correlated with AMH, but it accounts for only a portion of the variation seen in reproductive age African American women. PMID: 27114331
    34. AMH was inversely correlated with age in both the fertile and infertile populations and there was no significant difference between the fertile and infertile populations in terms of AMH. PMID: 27499425
    35. Data suggest that, in adolescents (as with adults) with PCOS (polycystic ovary syndrome), serum levels of 17-hydroxyprogesterone are variable as a characteristic of the disorder; 17-hydroxyprogesterone responsiveness to r-hCG (recombinant human chorionic gonadotropin, a fertility agent) is not correlated to serum levels of AMH (anti-Mullerian hormone). PMID: 27166718
    36. Anti-Mullerian hormone (AMH) and inhibin-A (INH-A) levels were found to be significantly higher in the polycystic ovary syndrome (PCOS) group compared to the controls. PMID: 27125339
    37. a significant positive correlation between serum asymmetric dimethylarginine and AMH levels in primary dysmenorrhea, is reported. PMID: 27523455
    38. Data suggest that the serum AMH level (a biomarker of ovarian reserve) in adolescent girls newly diagnosed with Hashimoto's thyroiditis (HT) is up-regulated compared to age- and BMI-matched control subjects; serum AMH levels are negatively correlated with serum biomarkers of oxidative stress in HT. PMID: 27343736
    39. Significantly lower serum AMH concentration was found in the regularly menstruating CKD women on hemodialysis in comparison with the healthy controls. 2. Serum AMH decreased significantly after successful kidney transplantation. PMID: 27553045
    40. AMH levels in serum and seminal plasma in healthy men and men with sperm pathology PMID: 27110929
    41. Genotyping of the AMH c.146G>T and AMHR2 -482A>G polymorphisms does not provide additional useful information as a predictor of ovarian reserve or ovarian response and treatment outcomes. PMID: 27142041
    42. The aim of this study was to investigate the density and distribution of single nucleotide polymorphisms (SNPs) anti-Mullerian hormone (AMH) and AMHRII receptors in cryptorchid patients. PMID: 27162065
    43. No evidence of significant associations of Ile49Ser and -482A>G with reproductive outcomes and polycystic ovary syndrome. PMID: 27832628
    44. Different phenotypes for polycystic ovary syndrome were identified with each related to different levels of anti-mullerian hormone. PMID: 26718304
    45. Data from a longitudinal, observational study suggest that, among women with type 1 diabetes, AMH levels in serum decline in manner similar to that previously reported in women without diabetes; thus, it is possible that AMH may be used to risk-stratify women with type 1 diabetes at risk for diminished ovarian reserve and poor reproductive outcomes in a similar manner as used in healthy women. PMID: 26798983
    46. Ile(49)Ser genotype not associated with estradiol levels, ovarian parameters, menstrual cycle length, or pregnancy outcomes in healthy Singapore women PMID: 26633196
    47. High serum Anti-Mullerian Hormone levels are associated with Polycystic Ovary Syndrome. PMID: 26761944
    48. Women with systemic lupus erythematosus demonstrated similar AMH levels as healthy controls, suggesting preserved ovarian reserve in this population. PMID: 26223296
    49. These findings contribute in clarifying the relationship between hormones regulating the early phase of steroidogenesis confirming that AMH is playing a suppressive role on CYP19A1 expression stimulated by gonadotropin in hGCs. Furthermore, a similar inhibitory effect for AMH was observed on P450scc gene expression when activated by gonadotropin treatment. PMID: 26631403
    50. The AMH and AMHRII gene polymorphisms were not found a significant difference in non-IR-PCOS and normal groups. To IR-PCOS women, genotypes of AMH were closely related to the serum levels of LH (P = 0.000), testosterone (P = 0.000) and HOMA-IR (P = 0.038), while in the non-IR-PCOS and normal groups, no relationship was found PMID: 26732661

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  • 相關(guān)疾病:
    Persistent Muellerian duct syndrome 1 (PMDS1)
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    TGF-beta family
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 464

    OMIM: 261550

    KEGG: hsa:268

    STRING: 9606.ENSP00000221496

    UniGene: Hs.112432



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