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Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

  • 中文名稱:
    人β淀粉樣蛋白1-40(Aβ1-40)酶聯免疫試劑盒
  • 貨號:
    CSB-E08299h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人β淀粉樣蛋白1-40(Aβ1-40)酶聯免疫試劑盒(CSB-E08299h)為雙抗夾心法ELISA試劑盒,定量檢測血清、組織勻漿、腦脊液(CSF)樣本中的Aβ1-40含量。Aβ1-40是β-淀粉樣蛋白的一種,在阿爾茨海默病研究中備受關注。其在大腦中異常聚集形成淀粉樣斑塊,是該病的病理特征之一。研究機制主要聚焦于探究其生成、清除過程的失衡,以及它對神經元毒性作用等方面,以尋找治療AD的新靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL,可廣泛應用于神經退行性疾病機制研究、藥物開發中淀粉樣蛋白調控效果評估、以及腦脊液生物標志物譜分析等科研領域,為探索Aβ1 - 40在病理過程中的分子作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
  • 縮寫:
    Aβ1-40
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, tissue homogenates, cerebrospinal fluid (CSF)
  • 檢測范圍:
    125 pg/mL-8000 pg/mL
  • 靈敏度:
    31.25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %80-92
    1:2Average %99
    Range %91-105
    1:4Average %95
    Range %92-98
    1:8Average %103
    Range %96-110
  • 回收率:
    The recovery of human Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9889-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.846 2.687 2.767 2.610
    40002.237 2.089 2.163 2.006
    20001.679 1.612 1.646 1.489
    10001.246 1.211 1.229 1.072
    5000.736 0.697 0.717 0.560
    2500.512 0.498 0.505 0.348
    1250.303 0.296 0.300 0.143
    00.160 0.154 0.157  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

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相關問答

 常見問題解答
Q:

We are interested in this kit.Could you let me know the information about the following ?
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

A:
Thanks for your inquiry.
Capture antibody:mouse monoclonal antibody;Detection antibody:goat polyclonal antibody.


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