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Human alpha-amylase(AMY1) ELISA Kit

  • 中文名稱：
    人α淀粉酶(AMY1)酶聯免疫試劑盒
  • 貨號：
    CSB-E14075h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人α淀粉酶(AMY1)酶聯免疫試劑盒（CSB-E14075h）為雙抗夾心法ELISA試劑盒，定量檢測血清、唾液、組織勻漿樣本中的AMY1含量。試劑盒檢測范圍為7.8 IU/mL-500 IU/mL，適用于科研領域中對消化酶活性動態的定量分析，如探究飲食習慣對唾液淀粉酶分泌的影響、疾病模型中胰腺外分泌功能評估，或通過組織勻漿分析特定器官的酶活性分布。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit
  • 縮寫：
    AMY1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, saliva, tissue homogenates
  • 檢測范圍：
    7.8 IU/mL-500 IU/mL
  • 靈敏度：
    1.95 IU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %81-93
    1:2Average %92
    Range %89-96
    1:4Average %90
    Range %84-96
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10096-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/mlOD1OD2AverageCorrected
    5002.991 2.789 2.890 2.788
    2502.607 2.604 2.606 2.504
    1252.254 2.213 2.234 2.132
    62.51.682 1.569 1.626 1.524
    31.20.931 0.924 0.928 0.826
    15.60.522 0.513 0.518 0.416
    7.80.291 0.288 0.290 0.188
    00.106 0.098 0.102 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

平均分:
5.0分 - 4 個評價

樣品類型：組織勻漿液 唾液

樣品信息：人

稀釋比：1:50

產品評價： 我用cSB-E14075h檢測人體唾液淀粉酶指標，標本品OD值0.065、0.102、0.209、0.324、0.524、0.924、1.578、2.14。

By 衛老師

樣品類型：其他樣品處理方法 唾液

樣品信息：人

稀釋比：沒有稀釋

產品評價： 我用cSB-E14075h檢測不孕人群唾液淀粉酶，標準品OD值范圍0.085、0.22、0.321、0.409、0.637、1.053、1.53、2.129。樣本OD值有幾個超出上限

By 衛老師

樣品類型：其他樣品處理方法 唾液

樣品信息：人

稀釋比：沒有稀釋

產品評價： 我用cSB-E14075h檢測不孕人群唾液淀粉酶濃度，標準品OD值：0.082、0.191、0.348、0.553、0.966、1.645、2.134、2.838，樣本濃度均在正常范圍內

By W老師

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