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  4. Human alcohol dehydrogenase,ADH ELISA Kit

Human alcohol dehydrogenase,ADH ELISA Kit

  • 中文名稱：
    人乙醇脫氫酶(ADH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11855h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人乙醇脫氫酶(ADH)酶聯(lián)免疫試劑盒（CSB-E11855h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的ADH1A含量。ADH1A是一個重要靶點。它在乙醇代謝等生理過程中發(fā)揮關(guān)鍵作用。研究機(jī)制方面，聚焦于其基因表達(dá)調(diào)控及蛋白功能。通過探究它與疾病的關(guān)聯(lián)，如酒精性肝病等，為開發(fā)針對性治療藥物和干預(yù)策略提供理論依據(jù)和研究方向。試劑盒檢測范圍為0.312 mU/mL-20 mU/mL，適用于酒精代謝機(jī)制研究、肝損傷模型評估、藥物或化合物對ADH活性調(diào)控作用分析等科研場景，特別針對體外培養(yǎng)細(xì)胞或動物實驗樣本中的ADH活性檢測需求開發(fā)，為代謝組學(xué)、毒理學(xué)及肝臟病理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
  • 縮寫：
    ADH1A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    0.312 mU/mL-20 mU/mL
  • 靈敏度：
    0.078 mU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %83
    Range %80-87
    1:2Average %102
    Range %97-107
    1:4Average %94
    Range %90-99
    1:8Average %86
    Range %83-89
  • 回收率：
    The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10299-105
    EDTA plasma (n=4)9790-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    202.294 2.156 2.225 2.107
    101.778 1.698 1.738 1.620
    51.253 1.240 1.247 1.129
    2.50.797 0.775 0.786 0.668
    1.250.485 0.468 0.477 0.359
    0.6250.362 0.375 0.369 0.251
    0.3120.256 0.262 0.259 0.141
    00.119 0.117 0.118
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 基因功能參考文獻(xiàn)：
    1. High ADH expression is associated with Prostate Cancer. PMID: 28870918
    2. Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
    3. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
    4. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
    5. high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
    6. common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
    7. High ADH1 is associated with endometrial cancer. PMID: 20872569
    8. ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
    9. Structural aspects that influence dimer-tetramer formation. PMID: 12081471
    10. GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
    11. The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
    12. Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
    13. Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 249

    OMIM: 103700

    KEGG: hsa:124

    STRING: 9606.ENSP00000209668

    UniGene: Hs.654433



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