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Human acylation stimulating protein(ASP) ELISA Kit

  • 中文名稱:
    人促酰化蛋白(ASP)酶聯免疫試劑盒
  • 貨號:
    CSB-E13474h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人促酰化蛋白(ASP)酶聯免疫試劑盒(CSB-E13474h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ASP含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于探索肥胖癥、2型糖尿病、代謝綜合征等疾病的分子機制研究,也可用于體外實驗模型中脂肪代謝相關藥物或營養干預的效果評估,為代謝領域基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    ASP
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %97
    Range %91-103
    1:200Average %89
    Range %84-95
    1:400Average %95
    Range %90-99
    1:800Average %84
    Range %80-88
  • 回收率:
    The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105100-110
    EDTA plasma (n=4)9085-95
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    102.527 2.452 2.490 2.390
    51.858 1.976 1.917 1.817
    2.51.204 1.294 1.249 1.149
    1.250.657 0.616 0.637 0.537
    0.6250.356 0.377 0.367 0.267
    0.3120.201 0.211 0.206 0.106
    0.1560.134 0.129 0.132 0.032
    00.098 0.101 0.100
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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