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Human activated coagulation factor X(FXa) ELISA kit

  • 中文名稱:
    人活化凝血因子X(FXa)酶聯免疫試劑盒
  • 貨號:
    CSB-E12696h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人活化凝血因子X(FXa)酶聯免疫試劑盒(CSB-E12696h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的FXa含量。FXa(因子 Xa)是凝血級聯反應中的關鍵酶。其背景在于在凝血過程里發揮核心作用,參與血栓形成。研究機制方面,聚焦于研發針對 FXa 的抑制劑,通過阻斷其活性來抑制凝血,進而用于預防和治療血栓相關疾病,降低血栓風險。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產品適用于凝血相關分子機制研究、血栓性疾病模型分析、抗凝劑藥效評估等科研領域,實驗數據穩定可靠,配套提供標準品、試劑及操作指南,滿足體外實驗需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    FXa
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %92-100
    1:2Average %92
    Range %87-96
    1:4Average %94
    Range %90-98
    1:8Average %89
    Range %83-96
  • 回收率:
    The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-102
    EDTA plasma (n=4)9690-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.536 2.598 2.567 2.405
    101.735 1.768 1.752 1.590
    51.036 1.098 1.067 0.905
    2.50.650 0.698 0.674 0.512
    1.250.446 0.432 0.439 0.277
    0.6250.342 0.358 0.350 0.188
    0.3120.240 0.261 0.251 0.089
    00.161 0.163 0.162  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

Is it possible to work with citrate-plasma instead of EDTA- or heparin-plasma?
Can you please provide exemplary data from plasma samples?

A:
Thanks for your inquiry.. It is recommended to detect the EDTA plasma sample with the kit.
Generally pls detect the normal healthy human sample. Pls let me know if you have any further questions. Thank you.


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