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Human Trypsin-3 (PRSS3) ELISA kit

  • 中文名稱:
    人胰蛋白酶3(PRSS3)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL018820HU
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人胰蛋白酶3(PRSS3)酶聯(lián)免疫試劑盒(CSB-EL018820HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PRSS3含量。PRSS3是頗具研究價值的靶點。它在生理與病理過程中發(fā)揮作用,與多種疾病發(fā)展相關(guān)。研究機制上,主要聚焦于其在疾病信號通路里的具體功能,以及與其他生物分子的相互作用,以探尋通過調(diào)控它來治療疾病的可能性。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,本產(chǎn)品適用于體外實驗環(huán)境下探索PRSS3在消化系統(tǒng)疾病、炎癥反應(yīng)或腫瘤微環(huán)境中的分子機制研究,其高靈敏度和寬線性范圍可滿足基礎(chǔ)科研中對細胞培養(yǎng)上清、動物模型血清或組織提取物的檢測需求,為研究PRSS3的表達調(diào)控、功能驗證及其作為潛在生物標(biāo)志物的探索提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Brain trypsinogen ELISA Kit; Mesotrypsin ELISA Kit; Mesotrypsinogen ELISA Kit; MTG ELISA Kit; Pancreatic trypsinogen III ELISA Kit; Protease, serine, 3 ELISA Kit; Protease, serine, 4 (trypsin 4, brain) ELISA Kit; PRSS3 ELISA Kit; PRSS4 ELISA Kit; Serine protease 3 ELISA Kit; Serine protease 4 ELISA Kit; T9 ELISA Kit; TRY3 ELISA Kit; TRY3_HUMAN ELISA Kit; TRY4 ELISA Kit; Trypsin 3 ELISA Kit; Trypsin III ELISA Kit; Trypsin IV ELISA Kit; Trypsin-3 ELISA Kit; Trypsinogen 4 ELISA Kit; Trypsinogen 5 ELISA Kit; Trypsinogen IV ELISA Kit
  • 縮寫:
    PRSS3
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 92
    Range % 87-96
    1:200 Average % 103
    Range % 98-107
    1:400 Average % 105
    Range % 101-109
    1:800 Average % 92
    Range % 86-98
  • 回收率:
    The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 86-98
    EDTA plasma (n=4) 97 91-103
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.584 2.687 2.636 2.552
    500 2.295 2.165 2.230 2.146
    250 1.594 1.523 1.559 1.475
    125 0.889 0.876 0.883 0.799
    62.5 0.441 0.454 0.448 0.364
    31.2 0.261 0.252 0.257 0.173
    15.6 0.143 0.148 0.146 0.062
    0 0.083 0.084 0.084  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.
  • 基因功能參考文獻:
    1. PRSS3 is downregulated by intragenic hypermethylation in HCC. * Epigenetic silencing of PRSS3 facilitates growth, migration, and invasion of HCC. * PRSS3 intragenic methylation has implication in diagnosis of HCC. PMID: 28844099
    2. PRSS3 acts as an oncogene in invasive ductal carcinoma of the breast development and progression PMID: 28423522
    3. Study developed a new high resolution crystal structure of mesotrypsin complexed with diminazene through a structure-based molecular docking screen that could help facilitate derivatization efforts, addressing current pan-inhibition of human trypsins through rigidification of diminazene to select for a conformation that maximizes interactions with the non-conserved Arg-193 residue. PMID: 28463992
    4. Data show that silencing tumor-endothelial cells (EC) for trypsinogen 4 accumulated tissue factor pathway inhibitor-2 (TFPI-2) in the matrix. PMID: 26318044
    5. These findings suggest that inhibitor cleavage represents a functional adaptation of mesotrypsin that may have evolved in response to positive selection pressure. PMID: 26175157
    6. High PRSS3 expression in EOC tissues was significantly associated with advanced FIGO stage and lymph node metastasis. PMID: 25735255
    7. Data suggest that mesotrypsin cleavage of Kunitz domains may contribute to cancer progression. PMID: 25301953
    8. Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
    9. extra-pancreatic trypsinogen 3 is produced by esophageal adenocarcinoma cells and activates PAR-2 in an autocrine manner. PMID: 24146905
    10. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
    11. Report PRSS3/mesotrypsin upregulation in breast cancer cells and identify CD109 as the functional proteolytic target of mesotrypsin. PMID: 20035377
    12. PRSS3 plays an important role in the progression, metastasis and prognosis of human pancreatic cancer. PMID: 20947888
    13. Investigation did not reveal an association between PRSS3 variants and chronic pancreatitis. PMID: 20484962
    14. Because mesotrypsin is resistant to naturally occurring trypsin inhibitors, confined expression of the isoforms of mesotrypsinogens and enteropeptidase may indicate that mesotrypsin is involved in keratinocyte terminal differentiation PMID: 19924134
    15. Processing by mesotrypsin may ablate the protease inhibitory function of APP/protease nexin 2 in vivo and may also modulate other activities of APP/protease nexin 2 that involve the Kunitz domain. PMID: 19920152
    16. X-ray structure in complex with the inhibitor benzamidine at 1.7 A resolution; crystal structure reveals basis for inhibitor resistance PMID: 11827488
    17. biological function of human mesotrypsin is digestive degradation of trypsin inhibitors PMID: 14507909
    18. The results classify E32del mesotrypsinogen as a frequent polymorphic variant, which is not associated with chronic alcoholic pancreatitis PMID: 15855826
    19. PRSS3 promoter methylation is associated with advanced bladder cancer PMID: 15987713
    20. we determined the promoter hypermethylation status of PRSS3 in a case series study of primary NSCLC, and found methylation of this gene to be common, occurring in 53% (86 of 166) of tumors examined. PMID: 16013053
    21. Results suggest that human trypsin 4 may be one of the candidate proteases involved in the pathomechanism of multiple sclerosis via cleavage of myelin basic protein. PMID: 16412431
    22. analysis of structural rearrangement during the acylation step in human trypsin 4 on 4-methylumbelliferyl 4-guanidinobenzoate substrate analogue PMID: 16492676
    23. mesotrypsin cannot activate pancreatic zymogens, but might activate certain proteinase-activated receptors because of its thrombin-like subsite specificity; alpha1AT Pittsburgh is an effective mesotrypsin inhibitor PMID: 16759229
    24. human trypsinogen 4 is widely but unevenly distributed in the human brain. It is localized in neurons and glial cells, predominantly in astrocytes & the extracellular matrix. PMID: 17406981
    25. trypsin IV and p23 are inhibitor-resistant trypsins that can cleave and activate PARs, causing PAR(1)- and PAR(2)-dependent inflammation and PAR(2)-dependent hyperalgesia. PMID: 17623652
    26. This study reveals enhanced mRNA expression of trypsinogen IV and SERT and a higher 5-HT content in the small intestine of IBS patients compared to healthy subjects. PMID: 18363639
    27. Absence of mesotrypsinogen gene (PRSS3) copy number variations in patients with chronic pancreatitis. PMID: 18665091
    28. Here, we report that nexin-1 inhibits trypsin-4, and forms stable complexes only with this trypsin-isoenzyme. This result suggests that nexin-1 could modulate trypsin activity in brain where both nexin-1 and trypsin-4 are expressed. PMID: 19249338

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Peptidase S1 family
  • 組織特異性:
    Detected in pancreas and pancreatic fluid (at protein level). Expressed in pancreas and brain. Detected in ileum.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 9486

    OMIM: 613578

    KEGG: hsa:5646

    STRING: 9606.ENSP00000354280

    UniGene: Hs.654513



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