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  4. Human Troponin T, fast skeletal muscle(TNNT3) ELISA kit

Human Troponin T, fast skeletal muscle(TNNT3) ELISA kit

  • 中文名稱：
    人肌鈣蛋白T，骨骼肌快肌(TNNT3)酶聯免疫試劑盒
  • 貨號：
    CSB-EL024017HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人肌鈣蛋白T，骨骼肌快肌(TNNT3)酶聯免疫試劑盒（CSB-EL024017HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNNT3含量。TNNT3是重要靶點，它是心肌肌鈣蛋白T的一種亞型。在研究機制上，它與心肌收縮功能緊密相關，其結構和功能變化會影響心肌收縮舒張。對它的深入研究有助于揭示心肌病等心臟疾病發病機制，為相關疾病診斷和治療提供新方向。試劑盒檢測范圍為7.81 pg/mL-500 pg/mL，該產品可應用于運動科學領域研究運動后骨骼肌損傷的生物標志物動態變化，或用于肌肉疾病模型中TNNT3表達調控機制的探索，亦支持基因編輯細胞或動物模型的功能驗證實驗，為骨骼肌生理及病理機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    AMCD2B ELISA Kit; Beta TnTF ELISA Kit; Beta-TnTF ELISA Kit; DA2B ELISA Kit; DKFZp779M2348 ELISA Kit; fast skeletal muscle ELISA Kit; Fast skeletal muscle troponin T ELISA Kit; FSSV ELISA Kit; fTnT ELISA Kit; TNNT 3 ELISA Kit; Tnnt3 ELISA Kit; TNNT3_HUMAN ELISA Kit; TnTf ELISA Kit; Troponin T ELISA Kit; Troponin T fast skeletal muscle ELISA Kit; Troponin T type 3 (skeletal fast) ELISA Kit; Troponin T type 3 ELISA Kit; Troponin T3 skeletal fast ELISA Kit
  • 縮寫：
    TNNT3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.81 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TNNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 98
    Range % 89-101
    1:2 Average % 107
    Range % 99-111
    1:4 Average % 92
    Range % 84-95
    1:8 Average % 99
    Range % 90-103
  • 回收率：
    The recovery of human TNNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 95-108
    EDTA plasma (n=4) 98 92-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.033 1.963 1.998 1.872
    250 1.731 1.778 1.755 1.629
    125 1.255 1.289 1.272 1.146
    62.5 0.596 0.634 0.615 0.489
    31.25 0.297 0.308 0.303 0.177
    15.62 0.189 0.197 0.193 0.067
    7.81 0.153 0.150 0.152 0.026
    0 0.128 0.124 0.126 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻：
    1. The presence of the p.(Arg63His) missense mutation at position 63 of TNNT3 was confirmed through direct cycle sequencing of genomic DNA in six affected South African family members for whom DNA had been archived. PMID: 26915936
    2. Data show that a missense mutation at nucleotide position 187 in exon 9 of the TNNT3 gene was identified in all affected individuals in the family. PMID: 21402185
    3. TNNT3 and LDB3 showed abnormal splicing and appeared more pronounced in myotonic dystrophies type 2 relative to myotonic dystrophies type 1. PMID: 20066428
    4. These results indicate that the f peptide confers an inhibitory effect on the biological function of fast skeletal troponin T and this can be correlated with changes in the Ca2+ regulation associated with development in fast skeletal muscle. PMID: 16081096
    5. Skeletal muscle contractile gene (TNNT3, MYH3, TPM2) mutations not found in vertical talus or clubfoot. PMID: 19142688
    6. Myotonic dystrophy type 2 muscles exhibited a higher degree of alternative splicing dysregulation for fast TnT transcripts when compared to myotonic dystrophy type 1 muscles. PMID: 19326042

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  • 相關疾病：
    Arthrogryposis, distal, 2B (DA2B)
  • 蛋白家族：
    Troponin T family
  • 組織特異性：
    In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
  • 數據庫鏈接：

    HGNC: 11950

    OMIM: 600692

    KEGG: hsa:7140

    STRING: 9606.ENSP00000278317

    UniGene: Hs.73454



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